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Cellosaurus DT40 (CVCL_0249)

[Text version]
Cell line name DT40
Synonyms DT-40; DT 40; DT40B; LSCC-DT40
Accession CVCL_0249
Resource Identification Initiative To cite this cell line use: DT40 (RRID:CVCL_0249)
Comments Group: Bird cell line.
Doubling time: ~48 hours (DSMZ=ACC-636); ~1 day (Note=Lot 02272006) (JCRB=JCRB9130).
Transformant: NCBI_TaxID; 11864; Avian leukosis virus (ALV).
Omics: Genomics; Whole genome sequencing.
Omics: Proteomics.
Cell type: B-cell; CL=CL_0000236.
Disease Chicken bursal lymphoma (NCIt: C134556)
Species of origin Gallus gallus (Chicken) (NCBI Taxonomy: 9031)
Breed/subspecies: Hy-Line SC.
Hierarchy Children:
CVCL_DD18 (D(Mh11)1)CVCL_9084 (D33-3)CVCL_9U92 (Delta-Rad51+hRad51-DT40)
CVCL_9U93 (Delta-Rad54+hRad54-DT40)CVCL_AS94 (Delta-Ubc9+cUbc9-DT40)CVCL_J437 (DT40 CL18)
CVCL_6D46 (DT40 Cre1)CVCL_6D43 (DT40(v-myb)delta-pol-beta)CVCL_6D45 (DT40(v-myb)delta-pol-beta-delta-pol-lambda)
CVCL_6D44 (DT40(v-myb)delta-pol-lambda)CVCL_6D42 (DT40(v-myb)WT)CVCL_1T23 (DT40-ATM(-/-))
CVCL_1T25 (DT40-BLM(-/-))CVCL_2H96 (DT40-BLM(-/-)-WRN(-/-))CVCL_1T38 (DT40-BLNK(-/-))
CVCL_4E62 (DT40-BLNK(-/-)-GRB2(-/-))CVCL_1T45 (DT40-BTK(-/-))CVCL_1T40 (DT40-CARD11(-/-))
CVCL_1T46 (DT40-CBL(-/-))CVCL_1U33 (DT40-GRAP(-/-))CVCL_1U32 (DT40-GRB2(-/-))
CVCL_4E68 (DT40-GRB2(-/-)-GRAP(-/-))CVCL_2G93 (DT40-INPP5D(-/-))CVCL_8B31 (DT40-ITRP1(-/-)-ITPR2(-/-))
CVCL_4634 (DT40-ITRP1(-/-)-ITPR2(-/-)-ITPR3(-/-))CVCL_8B32 (DT40-ITRP1(-/-)-ITPR3(-/-))CVCL_8B33 (DT40-ITRP2(-/-)-ITPR3(-/-))
CVCL_1T44 (DT40-LIG4(-/-))CVCL_1T41 (DT40-LYN(-/-))CVCL_2H33 (DT40-LYN(-/-)-SYK(-/-))
CVCL_1T50 (DT40-MAP3K7(-/-))CVCL_JE98 (DT40-MRE11(-/-) clone #190)CVCL_JE99 (DT40-MRE11(-/-) clone #194)
CVCL_4Z66 (DT40-MRE11(-/-)-XRCC6(-/-))CVCL_1T33 (DT40-NBN(-/-))CVCL_1T26 (DT40-ORAI1(-/-))
CVCL_1T27 (DT40-ORAI2(-/-))CVCL_1T39 (DT40-PIK3AP1(-/-))CVCL_4E64 (DT40-PIK3CA(-/-))
CVCL_1T47 (DT40-PLCG2(-/-))CVCL_1T32 (DT40-POLK(-/-))CVCL_4E66 (DT40-POLK(-/-)-XPA(-/-))
CVCL_1T42 (DT40-PRKCB(-/-))CVCL_1Y06 (DT40-PRKDC(-/-))CVCL_9Q56 (DT40-PRKDC(-/-)-XRCC6(-/-))
CVCL_2G90 (DT40-PTPN11(-/-))CVCL_2G91 (DT40-PTPN6(-/-))CVCL_1U31 (DT40-RAD18(-/-))
CVCL_4E67 (DT40-RAD18(-/-)-POLK(-/-))CVCL_4E65 (DT40-RAD18(-/-)-XPA(-/-))CVCL_X637 (DT40-RAD51B(-/-))
CVCL_X638 (DT40-RAD51C(-/-))CVCL_X639 (DT40-RAD51D(-/-))CVCL_X640 (DT40-RAD52(-/-))
CVCL_2H95 (DT40-RAD52(-/-)-XRCC3(-/-))CVCL_6D41 (DT40-RAD54B(-/-)-XRCC6(-/-))CVCL_1Y07 (DT40-RAD54L(-/-)-RAD54B(-/-))
CVCL_4W13 (DT40-RAD54L(-/-)-XRCC6(-/-))CVCL_1U38 (DT40-RASGRP1(-/-))CVCL_1U39 (DT40-RASGRP1(-/-)-RASGRP3(-/-))
CVCL_1U40 (DT40-RASGRP3(-/-))CVCL_1U34 (DT40-SHC1(-/-))CVCL_1U35 (DT40-SOS1(-/-))
CVCL_1U37 (DT40-SOS1(-/-)-SOS2(-/-))CVCL_1U36 (DT40-SOS2(-/-))CVCL_1T28 (DT40-STIM1(-/-))
CVCL_1T29 (DT40-STIM1(-/-)-STIM2(-/-))CVCL_1T30 (DT40-STIM2(-/-))CVCL_1T43 (DT40-SYK(-/-))
CVCL_2I01 (DT40-TRPC1(-/-))CVCL_1T49 (DT40-VAV3(-/-))CVCL_1T35 (DT40-WRN(-/-))
CVCL_1T36 (DT40-XRCC2(-/-))CVCL_2G89 (DT40-XRCC3(+/-))CVCL_1T37 (DT40-XRCC3(-/-))
CVCL_1T31 (DT40-XRCC6(-/-))CVCL_WK49 (iLite IL-2 Assay Ready Cells)CVCL_E7RR (iLite IL-23 Assay Ready Cells)
CVCL_E7RS (iLite Insulin Assay Ready Cells)CVCL_C0TX (JPR3)CVCL_1R68 (MSH6-1-[ClX3.16]DT40)
Sex of cell Female
Age at sampling 1D
Category Cancer cell line
Web pages Info; -; -; https://web.archive.org/web/20151216020045/http://www2.mrc-lmb.cam.ac.uk/groups/jes/pages/dt40.html
Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/d/cell-lines-detail-140.html
Publications

PubMed=2998040; DOI=10.1016/0042-6822(85)90312-5
Timothy W. Baba, Brett P. Giroir, Eric H. Humphries;
Cell lines derived from avian lymphomas exhibit two distinct phenotypes.
Virology 144:139-151(1985)

PubMed=18766641; DOI=10.1080/03079458708436402
Keyvan Nazerian;
An updated list of avian cell lines and transplantable tumours.
Avian Pathol. 16:527-544(1987)

PubMed=11226459; DOI=10.1016/S0022-1759(00)00333-1
Pernille Winding, Martin Werner Berchtold;
The chicken B cell line DT40: a novel tool for gene disruption experiments.
J. Immunol. Methods 249:1-16(2001)

PubMed=17675859; DOI=10.1159/000103179
Jurgen Bachl, Randolph B. Caldwell, Jean-Marie Buerstedde;
Biotechnology and the chicken B cell line DT40.
Cytogenet. Genome Res. 117:189-194(2007)

PubMed=19181659; DOI=10.1074/mcp.M800394-MCP200; PMCID=PMC2690488
Stephanie L. Hall, Svenja Hester, Julian L. Griffin, Kathryn S. Lilley, Antony Philip Jackson;
The organelle proteome of the DT40 lymphocyte cell line.
Mol. Cell. Proteomics 8:1295-1305(2009)

PubMed=25227228; DOI=10.1534/g3.114.013482; PMCID=PMC4232548
Janos Molnar, Adam Poti, Orsolya Pipek, Marcin Krzystanek, Nnennaya Kanu, Charles Swanton, Gabor E. Tusnady, Zoltan Szallasi, Istvan Csabai, David Szuts;
The genome of the chicken DT40 bursal lymphoma cell line.
G3 (Bethesda) 4:2231-2240(2014)

PubMed=26217713; DOI=10.1016/j.dib.2014.12.006; PMCID=PMC4509924
Johanna S. Rees, Kathryn S. Lilley, Antony Philip Jackson;
The chicken B-cell line DT40 proteome, beadome and interactomes.
Data Brief 3:29-33(2015)

Cross-references
Cell line collections (Providers) ATCC; CRL-2111
DSMZ; ACC-636
JCRB; JCRB9130
NCBI_Iran; C618
RCB; RCB1464
Cell line databases/resources CLO; CLO_0002837
CLO; CLO_0051524
MCCL; MCC:0000139
DSMZCellDive; ACC-636
Lonza; 267
TOKU-E; 1101
Anatomy/cell type resources BTO; BTO_0004814
Encyclopedic resources Wikidata; Q54831466
Experimental variables resources EFO; EFO_0006274
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number29