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Cellosaurus NIH 3T3 (CVCL_0594)

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Cell line name NIH 3T3
Synonyms NIH/3T3; NIH-3T3; NIH3T3; 3T3; 3T3NIH; 3T3-Swiss; Swiss-3T3; Swiss/3T3; Swiss 3T3; Swiss3T3
Accession CVCL_0594
Resource Identification Initiative To cite this cell line use: NIH 3T3 (RRID:CVCL_0594)
Comments Part of: ENCODE project mouse cell lines.
Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).
Doubling time: 21.3 +- 0.5 hours (PubMed=22619183); ~20 hours (DSMZ=ACC-59).
Omics: Transcriptomics; Microarray.
Omics: Variations; SNP array analysis.
Miscellaneous: PubMed=23430347 has a different value for STR 4-2 (19.3) than that of NIST (19.3,20.3) due to a change in the stutter rules (personal communication of Almeida, Jamie L.).
Miscellaneous: PubMed=23430347 has a different value for STR 6-4 (14.3) than that of NIST (15.3) due to a change in the marker motif (personal communication of Almeida, Jamie L.).
Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
Cell type: Fibroblast; CL=CL_0000057.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: NIH Swiss.
Hierarchy Children:
CVCL_M056 (3J1)CVCL_YY06 (3T3 rho-0#8)CVCL_A0VH (3T3-mB7-H4)
CVCL_1H10 (3T3-msCD40L)CVCL_1E11 (3T3.T4)CVCL_F962 (A4 [Mouse])
CVCL_D7C7 (Abeomics NIH 3T3 C3aR1)CVCL_4104 (Ampli-GPE)CVCL_9242 (ANN-1)
CVCL_0396 (B104-1-1)CVCL_C5QT (BPS Bioscience NIH 3T3 Gli-driven luciferase reporter)CVCL_2402 (C1I)
CVCL_A8QK (CD126)CVCL_KS69 (CellSensor dhfr(E2F)-bla NIH3T3)CVCL_KS54 (CellSensor Gli-bla NIH3T3)
CVCL_F498 (Cle-H3)CVCL_B952 (D4 [Mouse NIH 3T3 ERBB2 transfected])CVCL_2437 (Df)
CVCL_6254 (DHFR-G8)CVCL_2441 (E111)CVCL_6D48 (EGFR-T17)
CVCL_5741 (EJ-6-2-Bam-6a)CVCL_U422 (Flp-In-3T3)CVCL_WS34 (GhrasT-NIH/Swiss)
CVCL_8956 (GP+E-86)CVCL_8957 (GP+envAM-12)CVCL_D576 (HER14)
CVCL_D6C8 (HyCyte NIH 3T3 KO-mCul3)CVCL_E1MB (HyCyte NIH 3T3 KO-mImpdh2)CVCL_E1MC (HyCyte NIH 3T3 KO-mNsun6)
CVCL_4347 (II b)CVCL_6662 (K:Molv NIH/3T3)CVCL_E5ZT (LEADING LIGHT Wnt Reporter)
CVCL_D7AG (Leeporter NIH 3T3 IL-6 luciferase)CVCL_D7AH (Leeporter NIH 3T3 STAT3 luciferase)CVCL_KS91 (MEF/3T3 Tet-Off)
CVCL_U510 (MG87)CVCL_Y512 (MOP-6)CVCL_2617 (MOP-8)
CVCL_D900 (NEF)CVCL_5I45 (NIH 2/4)CVCL_QY95 (NIH 3T3 Atg5 KO)
CVCL_A0MM (NIH 3T3 Beta6)CVCL_HC50 (NIH 3T3 Duo Parental)CVCL_E6DP (NIH 3T3 EGFR)
CVCL_E6DQ (NIH 3T3 EGFR-A763_Y764insFQEA)CVCL_E6DR (NIH 3T3 EGFR-D770_N771insG)CVCL_E6DS (NIH 3T3 EGFR-D770_N771insSVD)
CVCL_E6DT (NIH 3T3 EGFR-Ex19del)CVCL_E6DU (NIH 3T3 EGFR-Ex19del/T790M)CVCL_E6DV (NIH 3T3 EGFR-H773_V774insNPH)
CVCL_E6DW (NIH 3T3 EGFR-H773_V774insPH)CVCL_E6DX (NIH 3T3 EGFR-V769_D770insASV)CVCL_E5L8 (NIH 3T3 hRRAS2 A158V)
CVCL_E5L9 (NIH 3T3 hRRAS2 A167T)CVCL_E5LA (NIH 3T3 hRRAS2 A70T)CVCL_E5LB (NIH 3T3 hRRAS2 D42Y)
CVCL_E5LC (NIH 3T3 hRRAS2 D44E)CVCL_E5LD (NIH 3T3 hRRAS2 D49Y)CVCL_E5LE (NIH 3T3 hRRAS2 D8N)
CVCL_E5LF (NIH 3T3 hRRAS2 E48D)CVCL_E5LG (NIH 3T3 hRRAS2 F204L)CVCL_E5LH (NIH 3T3 hRRAS2 G23A)
CVCL_E5LI (NIH 3T3 hRRAS2 G23C)CVCL_E5LJ (NIH 3T3 hRRAS2 G23S)CVCL_E5LK (NIH 3T3 hRRAS2 G24C)
CVCL_E5LL (NIH 3T3 hRRAS2 G24D)CVCL_E5LM (NIH 3T3 hRRAS2 G24V)CVCL_E5LN (NIH 3T3 hRRAS2 K14T)
CVCL_E5LP (NIH 3T3 hRRAS2 K159Q)CVCL_E5LQ (NIH 3T3 hRRAS2 K177T)CVCL_E5LR (NIH 3T3 hRRAS2 K53M)
CVCL_E5LS (NIH 3T3 hRRAS2 L112I)CVCL_E5LT (NIH 3T3 hRRAS2 M162V)CVCL_E5LU (NIH 3T3 hRRAS2 P45R)
CVCL_E5LV (NIH 3T3 hRRAS2 Q72H)CVCL_E5LW (NIH 3T3 hRRAS2 Q72L)CVCL_E5LX (NIH 3T3 hRRAS2 R117C)
CVCL_E5LY (NIH 3T3 hRRAS2 R135S)CVCL_E5LZ (NIH 3T3 hRRAS2 R147Q)CVCL_E5M0 (NIH 3T3 hRRAS2 R173W)
CVCL_E5M1 (NIH 3T3 hRRAS2 R63Q)CVCL_E5M2 (NIH 3T3 hRRAS2 WT)CVCL_E5M3 (NIH 3T3 hRRAS2 Y105C)
CVCL_A9G7 (NIH 3T3 IFT20-GFP)CVCL_ZD68 (NIH 3T3 Met-H1092L)CVCL_ZD69 (NIH 3T3 Met-H1092Y)
CVCL_ZD70 (NIH 3T3 Met-H1104D)CVCL_ZD71 (NIH 3T3 Met-L1193V)CVCL_ZD72 (NIH 3T3 Met-M1129T)
CVCL_ZD73 (NIH 3T3 Met-M1248T)CVCL_ZD74 (NIH 3T3 Met-V1090I)CVCL_ZD75 (NIH 3T3 Met-V1186L)
CVCL_ZD76 (NIH 3T3 Met-V1218I)CVCL_ZD67 (NIH 3T3 Met-WT)CVCL_ZD77 (NIH 3T3 Met-Y1228D)
CVCL_ZD78 (NIH 3T3 Met-Y1228H)CVCL_L994 (NIH 3T3 p-7 cl-3)CVCL_D675 (NIH 3T3-IL-7)
CVCL_5845 (NIH 3T3-ras)CVCL_9116 (NIH 3T3-TK-)CVCL_0R23 (NIH 3T3/DC-SIGN)
CVCL_LG16 (NIH 3T3/HER2-3(400))CVCL_C6XF (NIH 3T3/HNP)CVCL_0R24 (NIH 3T3/L-SIGN)
CVCL_C4BD (NIH 3T3/pL2neoSRalphaIIISFA-1)CVCL_7219 (NIH-3T3 4-2)CVCL_7220 (NIH-3T3 clone 5611)
CVCL_C4RN (NIH-3T3 Tsc2 KO)CVCL_L990 (NIH-3T3-A14)CVCL_E7X7 (NIH-3T3-eGFP-Smo)
CVCL_E7X5 (NIH-3T3-Flag-Smo 1.1)CVCL_E7X6 (NIH-3T3-Flag-Smo 1.7)CVCL_L991 (NIH-3T3-G185)
CVCL_E9X9 (NIH-3T3-V185)CVCL_7218 (NIH-3T3D4)CVCL_DX81 (NIH/3T3 Cas9)
CVCL_E5TR (NIH/3T3 MMCV-transformed clone MC315)CVCL_V360 (NIH/3T3 Tet-On 3G)CVCL_B6J0 (NIH/3T3-174)
CVCL_C8Y9 (NIH/3T3-Luc)CVCL_C9FL (NIH/3T3/Cas9-hyg)CVCL_E7UI (NIH3T3 Kif7 KO)
CVCL_L992 (NIH3T3-3)CVCL_VA35 (NIH3T3-47)CVCL_M025 (NIH3T3/13C7)
CVCL_L995 (NIH3T3/14-1)CVCL_4Y01 (NIH3T3/ATCC/CMV-Luc)CVCL_D2QR (NIH3T3/fPD1)
CVCL_D2QS (NIH3T3/fPDL1)CVCL_JY97 (NIH3T3/GFP)CVCL_KS45 (NIH3T3/NFkB-luc)
CVCL_D206 (NWT-21)CVCL_2151 (OMEGA-E)CVCL_2165 (Psi-2)
CVCL_W642 (Psi-AM22b)CVCL_4278 (Psi-CRE)CVCL_4279 (Psi-CRIP)
CVCL_LK41 (RL7)CVCL_9Y75 (Sb51)CVCL_D6UR (SFDH)
CVCL_E8AK (Shh Light)CVCL_2721 (Shh Light II)CVCL_0552 (Swiss-LAPA)
CVCL_E0ZU (Ubigene NIH/3T3 Bdnf KO)CVCL_E0ZV (Ubigene NIH/3T3 Dnm3os KO)CVCL_E0ZW (Ubigene NIH/3T3 Fabp4 KO)
CVCL_E0ZX (Ubigene NIH/3T3 Mecp2 KO)CVCL_E0ZY (Ubigene NIH/3T3 Pdk4 KO)CVCL_E0ZZ (Ubigene NIH/3T3 Ppp2r1b KO)
CVCL_E1A0 (Ubigene NIH/3T3 Prmt2 KO)CVCL_E1A1 (Ubigene NIH/3T3 Rock1 KO)CVCL_E1A2 (Ubigene NIH/3T3 Tfap2a KO)
CVCL_E1A3 (Ubigene NIH/3T3 Tns1 KO)CVCL_E1A4 (Ubigene NIH/3T3 Tsc2 KO)CVCL_E1A5 (Ubigene NIH/3T3 Usp20 KO)
CVCL_3433 (WOP)
Sex of cell Male
Age at sampling Embryo
Category Spontaneously immortalized cell line
STR profile Source(s): PubMed=23430347; PubMed=31220119; Ubigene=YC-A013

Markers:
Mouse STR 1-110
Mouse STR 1-213,17
Mouse STR 2-19
Mouse STR 3-214,15
Mouse STR 4-219.3,20.3
Mouse STR 5-514,15
Mouse STR 6-415.3
Mouse STR 6-712
Mouse STR 7-129
Mouse STR 8-115
Mouse STR 9-215,16
Mouse STR 11-215,17
Mouse STR 12-120
Mouse STR 13-116.2
Mouse STR 15-320.3
Mouse STR 17-213,14
Mouse STR 18-317,19
Mouse STR 19-211,12
Mouse STR X-125

Run an STR similarity search on this cell line
Web pages Info; -; -; https://nih3t3.com/
Info; ATCC; -; https://www.atcc.org/en/support/technical-support/faqs/atcc-ccl-92-vs-atcc-crl-1658
Info; ATCC; -; https://www.atcc.org/en/support/technical-support/faqs/confluence-for-atcc-crl-1658
Info; ATCC; -; https://www.atcc.org/en/support/technical-support/faqs/growth-rate-for-atcc-crl-1658
Info; COPE; -; http://www.cells-talk.com/index.php/page/copelibrary?key=3T3
Info; ENCODE; -; http://genome.ucsc.edu/ENCODE/protocols/cell/mouse/NIH-3T3_Stam_protocol.pdf
Info; HPA; -; https://www.proteinatlas.org/learn/cellines
Info; Wikipedia; -; https://en.wikipedia.org/wiki/3T3_cells
Publications

PubMed=4311790; DOI=10.1128/JVI.4.5.549-553.1969; PMCID=PMC375908
John L. Jainchill, Stuart A. Aaronson, George J. Todaro;
Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells.
J. Virol. 4:549-553(1969)

PubMed=4087463
Aikichi Iwamoto, Michiaki Masuda, Hiroshi Yoshikura;
Two NIH3T3 cell lines of different origins circulating in the world.
Jpn. J. Exp. Med. 55:129-131(1985)

PubMed=2463382; DOI=10.1128/JVI.63.2.753-758.1989; PMCID=PMC247747
Leda Helen Raptis, Joseph B. Bolen;
Polyomavirus transforms rat F111 and mouse NIH 3T3 cells by different mechanisms.
J. Virol. 63:753-758(1989)

PubMed=9753870
B. Jayashree, V. Visalakshi, K.R. Rajalakshmi, M.S. Sukumaran, M.S. Moni, Thangarajan Rajkumar, Sudha Deshmukh;
Characterization of mouse fibroblast (NIH3T3) and fibrosarcoma cell lines (WEHI-164 and MFS 8) using PMRS.
Indian J. Biochem. Biophys. 35:108-114(1998)

PubMed=17284666; DOI=10.1152/physiolgenomics.00199.2006
Gus J. Menger, Gregg Curtis Allen, Nichole Neuendorff, Sang-Soep Nahm, Terry L. Thomas, Vincent M. Cassone, David J. Earnest;
Circadian profiling of the transcriptome in NIH/3T3 fibroblasts: comparison with rhythmic gene expression in SCN2.2 cells and the rat SCN.
Physiol. Genomics 29:280-289(2007)

DOI=10.3917/bhesv.191.0045
Enrique Wulff Barreiro, Emilia Curras;
Mutation carriers: led by Spaniards in U.S. laboratories in the early 80s.
Bull. Hist. Epistem. Sci. Vie 19:45-65(2012)

PubMed=22619183; DOI=10.1002/btpr.1564
Annalaura Mancia, John T. Elliott, Michael Halter, Kiran Bhadriraju, Alessandro Tona, Tighe A. Spurlin, Bobby L. Middlebrooks, John E. Baatz, Gregory W. Warr, Anne L. Plant;
Quantitative methods to characterize morphological properties of cell lines.
Biotechnol. Prog. 28:1069-1078(2012)

PubMed=23321776; DOI=10.1369/0022155413476868; PMCID=PMC3621507
Christine Leibiger, Nadezda Kosyakova, Hasmik Mkrtchyan, Michael Glei, Vladimir A. Trifonov, Thomas Liehr;
First molecular cytogenetic high resolution characterization of the NIH 3T3 cell line by murine multicolor banding.
J. Histochem. Cytochem. 61:306-312(2013)

PubMed=23430347; DOI=10.1007/s10616-013-9545-7; PMCID=PMC3886540
Jamie L. Almeida, Carolyn R. Hill, Kenneth D. Cole;
Mouse cell line authentication.
Cytotechnology 66:133-147(2014)

PubMed=25277546; DOI=10.1186/1471-2164-15-847; PMCID=PMC4198738
John Paul Didion, Ryan J. Buus, Zohreh Naghashfar, David W. Threadgill, Herbert Carpenter Morse 3rd, Fernando Pardo-Manuel de Villena;
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847.1-847.11(2014)

PubMed=31220119; DOI=10.1371/journal.pone.0218412; PMCID=PMC6586308
Jamie L. Almeida, Aleksandra Dakic, Karin Kindig, Maikan Kone, Deborah Lee Dormady Letham, Scott Langdon, Ruth Peat, Jayamalini Holding-Pillai, Erin M. Hall, Mark Ladd, Megan D. Shaffer ...Show all 23 authors... , Heath Berg, Jin-Liang Li, Georges Wigger, Steve Lund, Carolyn R. Steffen, Barbara B. Fransway, Bob Geraghty, Manuela Natoli, Beth A. Bauer, Susanne M. Gollin, Dale W. Lewis, Yvonne A. Reid; Show fewer authors
Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.
PLoS ONE 14:e0218412.1-e0218412.24(2019)

PubMed=36078083; DOI=10.3390/cells11172677; PMCID=PMC9455036
Amir Mohammad Rahimi, Ming-Fang Cai, Sigrid Hoyer-Fender;
Heterogeneity of the NIH3T3 fibroblast cell line.
Cells 11:2677.1-2677.19(2022)

Cross-references
Cell line collections (Providers) ABM; T8981
AddexBio; P0011001/83
ATCC; CRL-1658
ATCC; CRL-6442 - Discontinued
BCRC; 60008
BCRJ; 0191
BEI_Resources; ARP-9946
CCLV; CCLV-RIE 0058
CCTCC; GDC0030
CLS; 400101
DSMZ; ACC-59
ECACC; 93061524
IBRC; C10100
ICLC; AL07001
IZSLER; BS CL 165
KCB; KCB 200645YJ
KCLB; 21658
NCBI_Iran; C156
RCB; RCB2767
TKG; TKG 0299
Ubigene; YC-A013
Cell line databases/resources CLO; CLO_0008172
CLO; CLO_0009227
CLO; CLO_0050548
MCCL; MCC:0000362
CLDB; cl3702
CLDB; cl3704
CLDB; cl3705
CLDB; cl3706
CLDB; cl3707
CLDB; cl3708
CLDB; cl3709
CLDB; cl3710
CLDB; cl3711
CLDB; cl4451
CLDB; cl7203
CCRID; 1101MOU-PUMC000018
CCRID; 1102MOU-NIFDC00034
CCRID; 3101MOUGNM6
CCRID; 3101MOUSCSP515
CCRID; 4201MOU-CCTCC00030
DSMZCellDive; ACC-59
Lonza; 115
TOKU-E; 2753
TOKU-E; 3547
Anatomy/cell type resources BTO; BTO_0000944
BTO; BTO_0005065
Biological sample resources BioSample; SAMN11397633
ENCODE; ENCBS117ENC
ENCODE; ENCBS990YBC
CRISP screens repositories BioGRID_ORCS_Cell_line; 301
Chemistry resources ChEMBL-Cells; CHEMBL3307716
ChEMBL-Targets; CHEMBL614822
PubChem_Cell_line; CVCL_0594
Encyclopedic resources Wikidata; Q4636420
Experimental variables resources EFO; EFO_0001222
Gene expression databases GEO; GSE5810
GEO; GSM1014177
Medical resources MeSH; D041681
Proteomic databases PRIDE; PXD001241
PRIDE; PXD004929
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number42