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Cellosaurus NIH 3T3 (CVCL_0594)

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Cell line name NIH 3T3
Synonyms NIH/3T3; NIH-3T3; NIH3T3; 3T3; 3T3NIH; 3T3-Swiss; Swiss-3T3; Swiss/3T3; Swiss 3T3; Swiss3T3
Accession CVCL_0594
Resource Identification Initiative To cite this cell line use: NIH 3T3 (RRID:CVCL_0594)
Comments Part of: ENCODE project mouse cell lines.
Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).
Doubling time: ~20 hours (DSMZ=ACC-59).
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Miscellaneous: PubMed=23430347 has a different value for STR 4-2 (19.3) than that of NIST (19.3,20.3) due to a change in the stutter rules (personal communication of Almeida J.L.).
Miscellaneous: PubMed=23430347 has a different value for STR 6-4 (14.3) than that of NIST (15.3) due to a change in the marker motif (personal communication of Almeida J.L.).
Derived from sampling site: Whole embryo. Cell type=Fibroblast.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: NIH Swiss.
Hierarchy Children:
CVCL_M056 (3J1)CVCL_YY06 (3T3 rho-0#8)CVCL_A0VH (3T3-mB7-H4)
CVCL_1H10 (3T3-msCD40L)CVCL_1E11 (3T3.T4)CVCL_F962 (A4 [Mouse])
CVCL_4104 (Ampli-GPE)CVCL_9242 (ANN-1)CVCL_0396 (B104-1-1)
CVCL_2402 (C1I)CVCL_A8QK (CD126)CVCL_KS69 (CellSensor dhfr(E2F)-bla NIH3T3)
CVCL_KS54 (CellSensor Gli-bla NIH3T3)CVCL_F498 (Cle-H3)CVCL_B952 (D4 [Mouse NIH 3T3 ERBB2 transfected])
CVCL_2437 (Df)CVCL_6254 (DHFR-G8)CVCL_2441 (E111)
CVCL_6D48 (EGFR-T17)CVCL_5741 (EJ-6-2-Bam-6a)CVCL_U422 (Flp-In-3T3)
CVCL_WS34 (GhrasT-NIH/Swiss)CVCL_C5QT (Gli reporter-NIH3T3)CVCL_8956 (GP+E-86)
CVCL_8957 (GP+envAM-12)CVCL_D576 (HER14)CVCL_4347 (II b)
CVCL_6662 (K:Molv NIH/3T3)CVCL_KS91 (MEF/3T3 Tet-Off)CVCL_U510 (MG87)
CVCL_Y512 (MOP-6)CVCL_2617 (MOP-8)CVCL_D900 (NEF)
CVCL_5I45 (NIH 2/4)CVCL_QY95 (NIH 3T3 Atg5 KO)CVCL_A0MM (NIH 3T3 Beta6)
CVCL_HC50 (NIH 3T3 Duo Parental)CVCL_A9G7 (NIH 3T3 IFT20-GFP)CVCL_ZD68 (NIH 3T3 Met-H1092L)
CVCL_ZD69 (NIH 3T3 Met-H1092Y)CVCL_ZD70 (NIH 3T3 Met-H1104D)CVCL_ZD71 (NIH 3T3 Met-L1193V)
CVCL_ZD72 (NIH 3T3 Met-M1129T)CVCL_ZD73 (NIH 3T3 Met-M1248T)CVCL_ZD74 (NIH 3T3 Met-V1090I)
CVCL_ZD75 (NIH 3T3 Met-V1186L)CVCL_ZD76 (NIH 3T3 Met-V1218I)CVCL_ZD67 (NIH 3T3 Met-WT)
CVCL_ZD77 (NIH 3T3 Met-Y1228D)CVCL_ZD78 (NIH 3T3 Met-Y1228H)CVCL_L994 (NIH 3T3 p-7 cl-3)
CVCL_D675 (NIH 3T3-IL-7)CVCL_5845 (NIH 3T3-ras)CVCL_9116 (NIH 3T3-TK-)
CVCL_0R23 (NIH 3T3/DC-SIGN)CVCL_LG16 (NIH 3T3/HER2-3(400))CVCL_C6XF (NIH 3T3/HNP)
CVCL_0R24 (NIH 3T3/L-SIGN)CVCL_C4BD (NIH 3T3/pL2neoSRalphaIIISFA-1)CVCL_7219 (NIH-3T3 4-2)
CVCL_7220 (NIH-3T3 clone 5611)CVCL_C4RN (NIH-3T3 Tsc2 KO)CVCL_L990 (NIH-3T3-A14)
CVCL_L991 (NIH-3T3-G185)CVCL_7218 (NIH-3T3D4)CVCL_V360 (NIH/3T3 Tet-On 3G)
CVCL_B6J0 (NIH/3T3-174)CVCL_L992 (NIH3T3-3)CVCL_VA35 (NIH3T3-47)
CVCL_M025 (NIH3T3/13C7)CVCL_L995 (NIH3T3/14-1)CVCL_4Y01 (NIH3T3/ATCC/CMV-Luc)
CVCL_JY97 (NIH3T3/GFP)CVCL_KS45 (NIH3T3/NFkB-luc)CVCL_D206 (NWT-21)
CVCL_2151 (OMEGA-E)CVCL_2165 (Psi-2)CVCL_W642 (Psi-AM22b)
CVCL_4278 (Psi-CRE)CVCL_4279 (Psi-CRIP)CVCL_LK41 (RL7)
CVCL_9Y75 (Sb51)CVCL_2721 (Shh Light II)CVCL_0552 (Swiss-LAPA)
CVCL_3433 (WOP)
Sex of cell Male
Age at sampling Embryo
Category Spontaneously immortalized cell line
STR profile Source(s): PubMed=23430347; PubMed=31220119

Markers:
Mouse STR 1-110
Mouse STR 1-213,17
Mouse STR 2-19
Mouse STR 3-214,15
Mouse STR 4-219.3,20.3
Mouse STR 5-514,15
Mouse STR 6-415.3
Mouse STR 6-712
Mouse STR 7-129
Mouse STR 8-115
Mouse STR 9-215,16
Mouse STR 11-215,17
Mouse STR 12-120
Mouse STR 13-116.2
Mouse STR 15-320.3
Mouse STR 17-213,14
Mouse STR 18-317,19
Mouse STR 19-211,12
Mouse STR X-125

Run an STR similarity search on this cell line
Web pages https://en.wikipedia.org/wiki/3T3_cells
http://www.nih3t3.com/
http://www.cells-talk.com/index.php/page/copelibrary?key=3T3
https://www.atcc.org/en/support/technical-support/faqs/atcc-ccl-92-vs-atcc-crl-1658
https://www.atcc.org/en/support/technical-support/faqs/growth-rate-for-atcc-crl-1658
https://www.atcc.org/en/support/technical-support/faqs/confluence-for-atcc-crl-1658
http://genome.ucsc.edu/ENCODE/protocols/cell/mouse/NIH-3T3_Stam_protocol.pdf
https://www.proteinatlas.org/learn/cellines
Publications

PubMed=4311790; DOI=10.1128/JVI.4.5.549-553.1969
Jainchill J.L., Aaronson S.A., Todaro G.J.
Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells.
J. Virol. 4:549-553(1969)

PubMed=4087463
Iwamoto A., Masuda M., Yoshikura H.
Two NIH3T3 cell lines of different origins circulating in the world.
Jpn. J. Exp. Med. 55:129-131(1985)

PubMed=2463382; DOI=10.1128/JVI.63.2.753-758.1989
Raptis L., Bolen J.B.
Polyomavirus transforms rat F111 and mouse NIH 3T3 cells by different mechanisms.
J. Virol. 63:753-758(1989)

PubMed=9753870
Jayashree B., Visalakshi V., Rajalakshmi K.R., Sukumaran M.S., Moni M.S., Rajkumar T., Deshmukh S.
Characterization of mouse fibroblast (NIH3T3) and fibrosarcoma cell lines (WEHI-164 and MFS 8) using PMRS.
Indian J. Biochem. Biophys. 35:108-114(1998)

PubMed=17284666; DOI=10.1152/physiolgenomics.00199.2006
Menger G.J., Allen G.C., Neuendorff N., Nahm S.-S., Thomas T.L., Cassone V.M., Earnest D.J.
Circadian profiling of the transcriptome in NIH/3T3 fibroblasts: comparison with rhythmic gene expression in SCN2.2 cells and the rat SCN.
Physiol. Genomics 29:280-289(2007)

DOI=10.3917/bhesv.191.0045
Barreiro E.W., Curras E.
Mutation carriers: led by Spaniards in U.S. laboratories in the early 80s.
Bull. Hist. Epistem. Sci. Vie 19:45-65(2012)

PubMed=23321776; DOI=10.1369/0022155413476868
Leibiger C., Kosyakova N., Mkrtchyan H., Glei M., Trifonov V.A., Liehr T.
First molecular cytogenetic high resolution characterization of the NIH 3T3 cell line by murine multicolor banding.
J. Histochem. Cytochem. 61:306-312(2013)

PubMed=23430347; DOI=10.1007/s10616-013-9545-7
Almeida J.L., Hill C.R., Cole K.D.
Mouse cell line authentication.
Cytotechnology 66:133-147(2014)

PubMed=25277546; DOI=10.1186/1471-2164-15-847
Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. III, Pardo-Manuel de Villena F.
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847.1-847.11(2014)

PubMed=31220119; DOI=10.1371/journal.pone.0218412
Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S., Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H., Li J.-L., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B., Natoli M., Bauer B., Gollin S.M., Lewis D.W., Reid Y.A.
Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.
PLoS ONE 14:E0218412-E0218412(2019)

PubMed=36078083; DOI=10.3390/cells11172677
Rahimi A.M., Cai M.-F., Hoyer-Fender S.
Heterogeneity of the NIH3T3 fibroblast cell line.
Cells 11:2677.1-2677.19(2022)

Cross-references
Cell line collections AddexBio; P0011001/83
ATCC; CRL-1658
ATCC; CRL-6442 - Discontinued
BCRC; 60008
BCRJ; 0191
CCLV; CCLV-RIE 0058
CCTCC; GDC0030
CLS; 400101
DSMZ; ACC-59
ECACC; 93061524
HIVReagentProgram; ARP-9946
IBRC; C10100
ICLC; AL07001
IZSLER; BS CL 165
KCB; KCB 200645YJ
KCLB; 21658
NCBI_Iran; C156
RCB; RCB2767
TKG; TKG 0299
Cell line databases/resources CLO; CLO_0008172
CLO; CLO_0009227
CLO; CLO_0050548
MCCL; MCC:0000362
CLDB; cl3702
CLDB; cl3704
CLDB; cl3705
CLDB; cl3706
CLDB; cl3707
CLDB; cl3708
CLDB; cl3709
CLDB; cl3710
CLDB; cl3711
CLDB; cl4451
CLDB; cl7203
CCRID; 1101MOU-PUMC000018
CCRID; 1102MOU-NIFDC00034
CCRID; 3101MOUGNM6
CCRID; 3101MOUSCSP515
CCRID; 4201MOU-CCTCC00030
DSMZCellDive; ACC-59
Lonza; 115
TOKU-E; 2753
TOKU-E; 3547
Anatomy/cell type resources BTO; BTO:0000944
BTO; BTO:0005065
Biological sample resources BioSample; SAMN11397633
ENCODE; ENCBS117ENC
ENCODE; ENCBS990YBC
Chemistry resources ChEMBL-Cells; CHEMBL3307716
ChEMBL-Targets; CHEMBL614822
PubChem_Cell_line; CVCL_0594
Encyclopedic resources Wikidata; Q4636420
Experimental variables resources EFO; EFO_0001222
Gene expression databases GEO; GSE5810
GEO; GSM1014177
Medical resources MeSH; D041681
Proteomic databases PRIDE; PXD001241
PRIDE; PXD004929
Entry history
Entry creation04-Apr-2012
Last entry update21-Mar-2023
Version number35