Cellosaurus cell line NIH 3T3 (CVCL

Cellosaurus NIH 3T3 (CVCL_0594)

[Text version]

Cell line name

NIH 3T3

Synonyms

NIH/3T3; NIH-3T3; NIH3T3; 3T3; 3T3NIH; 3T3-Swiss; Swiss-3T3; Swiss/3T3; Swiss 3T3; Swiss3T3

Accession

CVCL_0594

Resource Identification Initiative

To cite this cell line use: NIH 3T3 (RRID:CVCL_0594)

Comments

Part of: ENCODE project mouse cell lines.
Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).
Doubling time: 21.3 +- 0.5 hours (PubMed=22619183); ~20 hours (DSMZ=ACC-59).
Omics: Transcriptomics; Microarray.
Omics: Variations; SNP array analysis.
Miscellaneous: PubMed=23430347 has a different value for STR 4-2 (19.3) than that of NIST (19.3,20.3) due to a change in the stutter rules (personal communication of Almeida, Jamie L.).
Miscellaneous: PubMed=23430347 has a different value for STR 6-4 (14.3) than that of NIST (15.3) due to a change in the marker motif (personal communication of Almeida, Jamie L.).
Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
Cell type: Fibroblast; CL=CL_0000057.

Species of origin

Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: NIH Swiss.

Hierarchy

Children:

CVCL_M056 (3J1)	CVCL_YY06 (3T3 rho-0#8)	CVCL_A0VH (3T3-mB7-H4)
CVCL_1H10 (3T3-msCD40L)	CVCL_1E11 (3T3.T4)	CVCL_F962 (A4 [Mouse])
CVCL_D7C7 (Abeomics NIH 3T3 C3aR1)	CVCL_4104 (Ampli-GPE)	CVCL_9242 (ANN-1)
CVCL_0396 (B104-1-1)	CVCL_C5QT (BPS Bioscience NIH 3T3 Gli-driven luciferase reporter)	CVCL_2402 (C1I)
CVCL_A8QK (CD126)	CVCL_KS69 (CellSensor dhfr(E2F)-bla NIH3T3)	CVCL_KS54 (CellSensor Gli-bla NIH3T3)
CVCL_F498 (Cle-H3)	CVCL_B952 (D4 [Mouse NIH 3T3 ERBB2 transfected])	CVCL_2437 (Df)
CVCL_6254 (DHFR-G8)	CVCL_2441 (E111)	CVCL_6D48 (EGFR-T17)
CVCL_5741 (EJ-6-2-Bam-6a)	CVCL_U422 (Flp-In-3T3)	CVCL_WS34 (GhrasT-NIH/Swiss)
CVCL_8956 (GP+E-86)	CVCL_8957 (GP+envAM-12)	CVCL_D576 (HER14)
CVCL_D6C8 (HyCyte NIH 3T3 KO-mCul3)	CVCL_E1MB (HyCyte NIH 3T3 KO-mImpdh2)	CVCL_E1MC (HyCyte NIH 3T3 KO-mNsun6)
CVCL_4347 (II b)	CVCL_6662 (K:Molv NIH/3T3)	CVCL_E5ZT (LEADING LIGHT Wnt Reporter)
CVCL_D7AG (Leeporter NIH 3T3 IL-6 luciferase)	CVCL_D7AH (Leeporter NIH 3T3 STAT3 luciferase)	CVCL_KS91 (MEF/3T3 Tet-Off)
CVCL_U510 (MG87)	CVCL_Y512 (MOP-6)	CVCL_2617 (MOP-8)
CVCL_D900 (NEF)	CVCL_5I45 (NIH 2/4)	CVCL_QY95 (NIH 3T3 Atg5 KO)
CVCL_A0MM (NIH 3T3 Beta6)	CVCL_HC50 (NIH 3T3 Duo Parental)	CVCL_E6DP (NIH 3T3 EGFR)
CVCL_E6DQ (NIH 3T3 EGFR-A763_Y764insFQEA)	CVCL_E6DR (NIH 3T3 EGFR-D770_N771insG)	CVCL_E6DS (NIH 3T3 EGFR-D770_N771insSVD)
CVCL_E6DT (NIH 3T3 EGFR-Ex19del)	CVCL_E6DU (NIH 3T3 EGFR-Ex19del/T790M)	CVCL_E6DV (NIH 3T3 EGFR-H773_V774insNPH)
CVCL_E6DW (NIH 3T3 EGFR-H773_V774insPH)	CVCL_E6DX (NIH 3T3 EGFR-V769_D770insASV)	CVCL_E5L8 (NIH 3T3 hRRAS2 A158V)
CVCL_E5L9 (NIH 3T3 hRRAS2 A167T)	CVCL_E5LA (NIH 3T3 hRRAS2 A70T)	CVCL_E5LB (NIH 3T3 hRRAS2 D42Y)
CVCL_E5LC (NIH 3T3 hRRAS2 D44E)	CVCL_E5LD (NIH 3T3 hRRAS2 D49Y)	CVCL_E5LE (NIH 3T3 hRRAS2 D8N)
CVCL_E5LF (NIH 3T3 hRRAS2 E48D)	CVCL_E5LG (NIH 3T3 hRRAS2 F204L)	CVCL_E5LH (NIH 3T3 hRRAS2 G23A)
CVCL_E5LI (NIH 3T3 hRRAS2 G23C)	CVCL_E5LJ (NIH 3T3 hRRAS2 G23S)	CVCL_E5LK (NIH 3T3 hRRAS2 G24C)
CVCL_E5LL (NIH 3T3 hRRAS2 G24D)	CVCL_E5LM (NIH 3T3 hRRAS2 G24V)	CVCL_E5LN (NIH 3T3 hRRAS2 K14T)
CVCL_E5LP (NIH 3T3 hRRAS2 K159Q)	CVCL_E5LQ (NIH 3T3 hRRAS2 K177T)	CVCL_E5LR (NIH 3T3 hRRAS2 K53M)
CVCL_E5LS (NIH 3T3 hRRAS2 L112I)	CVCL_E5LT (NIH 3T3 hRRAS2 M162V)	CVCL_E5LU (NIH 3T3 hRRAS2 P45R)
CVCL_E5LV (NIH 3T3 hRRAS2 Q72H)	CVCL_E5LW (NIH 3T3 hRRAS2 Q72L)	CVCL_E5LX (NIH 3T3 hRRAS2 R117C)
CVCL_E5LY (NIH 3T3 hRRAS2 R135S)	CVCL_E5LZ (NIH 3T3 hRRAS2 R147Q)	CVCL_E5M0 (NIH 3T3 hRRAS2 R173W)
CVCL_E5M1 (NIH 3T3 hRRAS2 R63Q)	CVCL_E5M2 (NIH 3T3 hRRAS2 WT)	CVCL_E5M3 (NIH 3T3 hRRAS2 Y105C)
CVCL_A9G7 (NIH 3T3 IFT20-GFP)	CVCL_ZD68 (NIH 3T3 Met-H1092L)	CVCL_ZD69 (NIH 3T3 Met-H1092Y)
CVCL_ZD70 (NIH 3T3 Met-H1104D)	CVCL_ZD71 (NIH 3T3 Met-L1193V)	CVCL_ZD72 (NIH 3T3 Met-M1129T)
CVCL_ZD73 (NIH 3T3 Met-M1248T)	CVCL_ZD74 (NIH 3T3 Met-V1090I)	CVCL_ZD75 (NIH 3T3 Met-V1186L)
CVCL_ZD76 (NIH 3T3 Met-V1218I)	CVCL_ZD67 (NIH 3T3 Met-WT)	CVCL_ZD77 (NIH 3T3 Met-Y1228D)
CVCL_ZD78 (NIH 3T3 Met-Y1228H)	CVCL_L994 (NIH 3T3 p-7 cl-3)	CVCL_D675 (NIH 3T3-IL-7)
CVCL_5845 (NIH 3T3-ras)	CVCL_9116 (NIH 3T3-TK-)	CVCL_0R23 (NIH 3T3/DC-SIGN)
CVCL_LG16 (NIH 3T3/HER2-3(400))	CVCL_C6XF (NIH 3T3/HNP)	CVCL_0R24 (NIH 3T3/L-SIGN)
CVCL_C4BD (NIH 3T3/pL2neoSRalphaIIISFA-1)	CVCL_7219 (NIH-3T3 4-2)	CVCL_7220 (NIH-3T3 clone 5611)
CVCL_C4RN (NIH-3T3 Tsc2 KO)	CVCL_L990 (NIH-3T3-A14)	CVCL_E7X7 (NIH-3T3-eGFP-Smo)
CVCL_E7X5 (NIH-3T3-Flag-Smo 1.1)	CVCL_E7X6 (NIH-3T3-Flag-Smo 1.7)	CVCL_L991 (NIH-3T3-G185)
CVCL_7218 (NIH-3T3D4)	CVCL_DX81 (NIH/3T3 Cas9)	CVCL_E5TR (NIH/3T3 MMCV-transformed clone MC315)
CVCL_V360 (NIH/3T3 Tet-On 3G)	CVCL_B6J0 (NIH/3T3-174)	CVCL_C8Y9 (NIH/3T3-Luc)
CVCL_C9FL (NIH/3T3/Cas9-hyg)	CVCL_E7UI (NIH3T3 Kif7 KO)	CVCL_L992 (NIH3T3-3)
CVCL_VA35 (NIH3T3-47)	CVCL_M025 (NIH3T3/13C7)	CVCL_L995 (NIH3T3/14-1)
CVCL_4Y01 (NIH3T3/ATCC/CMV-Luc)	CVCL_D2QR (NIH3T3/fPD1)	CVCL_D2QS (NIH3T3/fPDL1)
CVCL_JY97 (NIH3T3/GFP)	CVCL_KS45 (NIH3T3/NFkB-luc)	CVCL_D206 (NWT-21)
CVCL_2151 (OMEGA-E)	CVCL_2165 (Psi-2)	CVCL_W642 (Psi-AM22b)
CVCL_4278 (Psi-CRE)	CVCL_4279 (Psi-CRIP)	CVCL_LK41 (RL7)
CVCL_9Y75 (Sb51)	CVCL_D6UR (SFDH)	CVCL_E8AK (Shh Light)
CVCL_2721 (Shh Light II)	CVCL_0552 (Swiss-LAPA)	CVCL_E0ZU (Ubigene NIH/3T3 Bdnf KO)
CVCL_E0ZV (Ubigene NIH/3T3 Dnm3os KO)	CVCL_E0ZW (Ubigene NIH/3T3 Fabp4 KO)	CVCL_E0ZX (Ubigene NIH/3T3 Mecp2 KO)
CVCL_E0ZY (Ubigene NIH/3T3 Pdk4 KO)	CVCL_E0ZZ (Ubigene NIH/3T3 Ppp2r1b KO)	CVCL_E1A0 (Ubigene NIH/3T3 Prmt2 KO)
CVCL_E1A1 (Ubigene NIH/3T3 Rock1 KO)	CVCL_E1A2 (Ubigene NIH/3T3 Tfap2a KO)	CVCL_E1A3 (Ubigene NIH/3T3 Tns1 KO)
CVCL_E1A4 (Ubigene NIH/3T3 Tsc2 KO)	CVCL_E1A5 (Ubigene NIH/3T3 Usp20 KO)	CVCL_3433 (WOP)

Sex of cell

Male

Age at sampling

Embryo

Category

Spontaneously immortalized cell line

STR profile

Source(s): PubMed=23430347; PubMed=31220119; Ubigene=YC-A013

Markers:

Mouse STR 1-1	10
Mouse STR 1-2	13,17
Mouse STR 2-1	9
Mouse STR 3-2	14,15
Mouse STR 4-2	19.3,20.3
Mouse STR 5-5	14,15
Mouse STR 6-4	15.3
Mouse STR 6-7	12
Mouse STR 7-1	29
Mouse STR 8-1	15
Mouse STR 9-2	15,16
Mouse STR 11-2	15,17
Mouse STR 12-1	20
Mouse STR 13-1	16.2
Mouse STR 15-3	20.3
Mouse STR 17-2	13,14
Mouse STR 18-3	17,19
Mouse STR 19-2	11,12
Mouse STR X-1	25

Web pages

Info; -; -; https://nih3t3.com/
Info; ATCC; -; https://www.atcc.org/en/support/technical-support/faqs/atcc-ccl-92-vs-atcc-crl-1658
Info; ATCC; -; https://www.atcc.org/en/support/technical-support/faqs/confluence-for-atcc-crl-1658
Info; ATCC; -; https://www.atcc.org/en/support/technical-support/faqs/growth-rate-for-atcc-crl-1658
Info; COPE; -; http://www.cells-talk.com/index.php/page/copelibrary?key=3T3
Info; ENCODE; -; http://genome.ucsc.edu/ENCODE/protocols/cell/mouse/NIH-3T3_Stam_protocol.pdf
Info; HPA; -; https://www.proteinatlas.org/learn/cellines
Info; Wikipedia; -; https://en.wikipedia.org/wiki/3T3_cells

Publications

PubMed=4311790; DOI=10.1128/JVI.4.5.549-553.1969; PMCID=PMC375908
Jainchill J.L., Aaronson S.A., Todaro G.J.
Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells.
J. Virol. 4:549-553(1969)

PubMed=4087463
Iwamoto A., Masuda M., Yoshikura H.
Two NIH3T3 cell lines of different origins circulating in the world.
Jpn. J. Exp. Med. 55:129-131(1985)

PubMed=2463382; DOI=10.1128/JVI.63.2.753-758.1989; PMCID=PMC247747
Raptis L.H., Bolen J.B.
Polyomavirus transforms rat F111 and mouse NIH 3T3 cells by different mechanisms.
J. Virol. 63:753-758(1989)

PubMed=9753870
Jayashree B., Visalakshi V., Rajalakshmi K.R., Sukumaran M.S., Moni M.S., Rajkumar T., Deshmukh S.
Characterization of mouse fibroblast (NIH3T3) and fibrosarcoma cell lines (WEHI-164 and MFS 8) using PMRS.
Indian J. Biochem. Biophys. 35:108-114(1998)

PubMed=17284666; DOI=10.1152/physiolgenomics.00199.2006
Menger G.J., Allen G.C., Neuendorff N., Nahm S.-S., Thomas T.L., Cassone V.M., Earnest D.J.
Circadian profiling of the transcriptome in NIH/3T3 fibroblasts: comparison with rhythmic gene expression in SCN2.2 cells and the rat SCN.
Physiol. Genomics 29:280-289(2007)

DOI=10.3917/bhesv.191.0045
Barreiro E.W., Curras E.
Mutation carriers: led by Spaniards in U.S. laboratories in the early 80s.
Bull. Hist. Epistem. Sci. Vie 19:45-65(2012)

PubMed=22619183; DOI=10.1002/btpr.1564
Mancia A., Elliott J.T., Halter M., Bhadriraju K., Tona A., Spurlin T.A., Middlebrooks B.L., Baatz J.E., Warr G.W., Plant A.L.
Quantitative methods to characterize morphological properties of cell lines.
Biotechnol. Prog. 28:1069-1078(2012)

PubMed=23321776; DOI=10.1369/0022155413476868; PMCID=PMC3621507
Leibiger C., Kosyakova N., Mkrtchyan H., Glei M., Trifonov V.A., Liehr T.
First molecular cytogenetic high resolution characterization of the NIH 3T3 cell line by murine multicolor banding.
J. Histochem. Cytochem. 61:306-312(2013)

PubMed=23430347; DOI=10.1007/s10616-013-9545-7; PMCID=PMC3886540
Almeida J.L., Hill C.R., Cole K.D.
Mouse cell line authentication.
Cytotechnology 66:133-147(2014)

PubMed=25277546; DOI=10.1186/1471-2164-15-847; PMCID=PMC4198738
Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. 3rd, Pardo-Manuel de Villena F.
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847.1-847.11(2014)

PubMed=31220119; DOI=10.1371/journal.pone.0218412; PMCID=PMC6586308
Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S., Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H., Li J.-L., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B., Natoli M., Bauer B.A., Gollin S.M., Lewis D.W., Reid Y.A.
Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.
PLoS ONE 14:e0218412.1-e0218412.24(2019)

PubMed=36078083; DOI=10.3390/cells11172677; PMCID=PMC9455036
Rahimi A.M., Cai M.-F., Hoyer-Fender S.
Heterogeneity of the NIH3T3 fibroblast cell line.
Cells 11:2677.1-2677.19(2022)

Cross-references

Cell line collections (Providers)

ABM; T8981
AddexBio; P0011001/83
ATCC; CRL-1658
ATCC; CRL-6442 - Discontinued
BCRC; 60008
BCRJ; 0191
BEI_Resources; ARP-9946
CCLV; CCLV-RIE 0058
CCTCC; GDC0030
CLS; 400101
DSMZ; ACC-59
ECACC; 93061524
IBRC; C10100
ICLC; AL07001
IZSLER; BS CL 165
KCB; KCB 200645YJ
KCLB; 21658
NCBI_Iran; C156
RCB; RCB2767
TKG; TKG 0299
Ubigene; YC-A013

Cell line databases/resources

CLO; CLO_0008172
CLO; CLO_0009227
CLO; CLO_0050548
MCCL; MCC:0000362
CLDB; cl3702
CLDB; cl3704
CLDB; cl3705
CLDB; cl3706
CLDB; cl3707
CLDB; cl3708
CLDB; cl3709
CLDB; cl3710
CLDB; cl3711
CLDB; cl4451
CLDB; cl7203
CCRID; 1101MOU-PUMC000018
CCRID; 1102MOU-NIFDC00034
CCRID; 3101MOUGNM6
CCRID; 3101MOUSCSP515
CCRID; 4201MOU-CCTCC00030
DSMZCellDive; ACC-59
Lonza; 115
TOKU-E; 2753
TOKU-E; 3547

Anatomy/cell type resources

BTO; BTO_0000944
BTO; BTO_0005065

Biological sample resources

BioSample; SAMN11397633
ENCODE; ENCBS117ENC
ENCODE; ENCBS990YBC

CRISP screens repositories

BioGRID_ORCS_Cell_line; 301

Chemistry resources

ChEMBL-Cells; CHEMBL3307716
ChEMBL-Targets; CHEMBL614822
PubChem_Cell_line; CVCL_0594

Encyclopedic resources

Wikidata; Q4636420

Experimental variables resources

EFO; EFO_0001222

Gene expression databases

GEO; GSE5810
GEO; GSM1014177

Medical resources

MeSH; D041681

Proteomic databases

PRIDE; PXD001241
PRIDE; PXD004929

Entry history

Entry creation

04-Apr-2012

Last entry update

10-Apr-2025

Version number