Cellosaurus logo
expasy logo

Cellosaurus Shh Light II (CVCL_2721)

[Text version]
Cell line name Shh Light II
Synonyms Shh-Light II; Shh Light 2; Shh LIGHT 2; Shh-LIGHT2; Shh Light2; Shh-Light2; Shh-L2
Accession CVCL_2721
Resource Identification Initiative To cite this cell line use: Shh Light II (RRID:CVCL_2721)
Comments Characteristics: Useful for the quantitative assay of biologically active hedgehog proteins (PubMed=10984056).
Characteristics: Transfected with a GLI-responsive firefly luciferase reporter as well as a constitutive (TK promoter driven) Renilla-luciferase reporter (PubMed=10984056).
Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Firefly luciferase.
Genetic integration: Method=Transfection; Gene=UniProtKB; P27652; Renilla-type luciferase.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
Cell type: Fibroblast; CL=CL_0000057.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: NIH Swiss.
Hierarchy Parent: CVCL_0594 (NIH 3T3)
Sex of cell Male
Age at sampling Embryo
Category Spontaneously immortalized cell line
Web pages Info; IdeaConnection; -; https://www.ideaconnection.com/patents/6088-Shh-Light2-Cells.html
Provider; JHTV; C04409; https://jhu.technologypublisher.com/technology/16328
Publications

PubMed=10984056; DOI=10.1038/35023008
Taipale J., Chen J.K., Cooper M.K., Wang B.-L., Mann R.K., Milenkovic L., Scott M.P., Beachy P.A.
Effects of oncogenic mutations in Smoothened and Patched can be reversed by cyclopamine.
Nature 406:1005-1009(2000)

Cross-references
Cell line collections (Providers) ATCC; CRL-2795 - Discontinued
ATCC; JHU-68 - Discontinued
Cell line databases/resources CLO; CLO_0009010
Chemistry resources ChEMBL-Cells; CHEMBL3430863
ChEMBL-Targets; CHEMBL3430862
PubChem_Cell_line; CVCL_2721
Encyclopedic resources Wikidata; Q54953329
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number15