ID   Shh Light II
AC   CVCL_2721
SY   Shh-Light II; Shh Light 2; Shh LIGHT 2; Shh-LIGHT2; Shh Light2; Shh-Light2; Shh-L2
DR   CLO; CLO_0009010
DR   ATCC; CRL-2795
DR   ATCC; JHU-68
DR   ChEMBL-Cells; CHEMBL3430863
DR   ChEMBL-Targets; CHEMBL3430862
DR   PubChem_Cell_line; CVCL_2721
DR   Wikidata; Q54953329
RX   PubMed=10984056;
WW   Info; IdeaConnection; -; https://www.ideaconnection.com/patents/6088-Shh-Light2-Cells.html
WW   Provider; JHTV; C04409; https://jhu.technologypublisher.com/technology/16328
CC   Characteristics: Useful for the quantitative assay of biologically active hedgehog proteins (PubMed=10984056).
CC   Characteristics: Transfected with a GLI-responsive firefly luciferase reporter as well as a constitutive (TK promoter driven) Renilla-luciferase reporter (PubMed=10984056).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Firefly luciferase.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P27652; Renilla-type luciferase.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Discontinued: ATCC; CRL-2795; true.
CC   Discontinued: ATCC; JHU-68; true.
CC   Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
CC   Cell type: Fibroblast; CL=CL_0000057.
CC   Breed/subspecies: NIH Swiss.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_0594 ! NIH 3T3
SX   Male
AG   Embryo
CA   Spontaneously immortalized cell line
DT   Created: 04-04-12; Last updated: 10-04-25; Version: 15
//
RX   PubMed=10984056; DOI=10.1038/35023008;
RA   Taipale J., Chen J.K., Cooper M.K., Wang B.-L., Mann R.K., Milenkovic L.,
RA   Scott M.P., Beachy P.A.;
RT   "Effects of oncogenic mutations in Smoothened and Patched can be
RT   reversed by cyclopamine.";
RL   Nature 406:1005-1009(2000).
//