ID   NIH 3T3
AC   CVCL_0594
SY   NIH/3T3; NIH-3T3; NIH3T3; 3T3; 3T3NIH; 3T3-Swiss; Swiss-3T3; Swiss/3T3; Swiss 3T3; Swiss3T3
DR   BTO; BTO:0000944
DR   BTO; BTO:0005065
DR   CLO; CLO_0008172
DR   CLO; CLO_0009227
DR   CLO; CLO_0050548
DR   EFO; EFO_0001222
DR   MCCL; MCC:0000362
DR   CLDB; cl3702
DR   CLDB; cl3704
DR   CLDB; cl3705
DR   CLDB; cl3706
DR   CLDB; cl3707
DR   CLDB; cl3708
DR   CLDB; cl3709
DR   CLDB; cl3710
DR   CLDB; cl3711
DR   CLDB; cl4451
DR   CLDB; cl7203
DR   AddexBio; P0011001/83
DR   ATCC; CRL-1658
DR   ATCC; CRL-6442
DR   BCRC; 60008
DR   BCRJ; 0191
DR   BioGRID_ORCS_Cell_line; 301
DR   BioSample; SAMN11397633
DR   CCLV; CCLV-RIE 0058
DR   CCRID; 1101MOU-PUMC000018
DR   CCRID; 1102MOU-NIFDC00034
DR   CCRID; 3101MOUGNM6
DR   CCRID; 3101MOUSCSP515
DR   CCRID; 4201MOU-CCTCC00030
DR   CCTCC; GDC0030
DR   ChEMBL-Cells; CHEMBL3307716
DR   ChEMBL-Targets; CHEMBL614822
DR   CLS; 400101
DR   DSMZ; ACC-59
DR   DSMZCellDive; ACC-59
DR   ECACC; 93061524
DR   ENCODE; ENCBS117ENC
DR   ENCODE; ENCBS990YBC
DR   GEO; GSE5810
DR   GEO; GSM1014177
DR   HIVReagentProgram; ARP-9946
DR   IBRC; C10100
DR   ICLC; AL07001
DR   IZSLER; BS CL 165
DR   KCB; KCB 200645YJ
DR   KCLB; 21658
DR   Lonza; 115
DR   MeSH; D041681
DR   NCBI_Iran; C156
DR   PRIDE; PXD001241
DR   PRIDE; PXD004929
DR   PubChem_Cell_line; CVCL_0594
DR   RCB; RCB2767
DR   TKG; TKG 0299
DR   TOKU-E; 2753
DR   TOKU-E; 3547
DR   Ubigene; YC-A013
DR   Wikidata; Q4636420
RX   DOI=10.3917/bhesv.191.0045;
RX   PubMed=2463382;
RX   PubMed=4087463;
RX   PubMed=4311790;
RX   PubMed=9753870;
RX   PubMed=17284666;
RX   PubMed=22619183;
RX   PubMed=23321776;
RX   PubMed=23430347;
RX   PubMed=25277546;
RX   PubMed=31220119;
RX   PubMed=36078083;
WW   https://en.wikipedia.org/wiki/3T3_cells
WW   http://www.nih3t3.com/
WW   http://www.cells-talk.com/index.php/page/copelibrary?key=3T3
WW   https://www.atcc.org/en/support/technical-support/faqs/atcc-ccl-92-vs-atcc-crl-1658
WW   https://www.atcc.org/en/support/technical-support/faqs/growth-rate-for-atcc-crl-1658
WW   https://www.atcc.org/en/support/technical-support/faqs/confluence-for-atcc-crl-1658
WW   http://genome.ucsc.edu/ENCODE/protocols/cell/mouse/NIH-3T3_Stam_protocol.pdf
WW   https://www.proteinatlas.org/learn/cellines
CC   Part of: ENCODE project mouse cell lines.
CC   Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).
CC   Doubling time: 21.3 +- 0.5 hours (PubMed=22619183); ~20 hours (DSMZ=ACC-59).
CC   Omics: SNP array analysis.
CC   Omics: Transcriptome analysis by microarray.
CC   Miscellaneous: PubMed=23430347 has a different value for STR 4-2 (19.3) than that of NIST (19.3,20.3) due to a change in the stutter rules (personal communication of Almeida J.L.).
CC   Miscellaneous: PubMed=23430347 has a different value for STR 6-4 (14.3) than that of NIST (15.3) due to a change in the marker motif (personal communication of Almeida J.L.).
CC   Discontinued: ATCC; CRL-6442; true.
CC   Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
CC   Cell type: Fibroblast; CL=CL_0000057.
CC   Breed/subspecies: NIH Swiss.
ST   Source(s): PubMed=23430347; PubMed=31220119; Ubigene
ST   Mouse STR 1-1: 10
ST   Mouse STR 1-2: 13,17
ST   Mouse STR 11-2: 15,17
ST   Mouse STR 12-1: 20
ST   Mouse STR 13-1: 16.2
ST   Mouse STR 15-3: 20.3
ST   Mouse STR 17-2: 13,14
ST   Mouse STR 18-3: 17,19
ST   Mouse STR 19-2: 11,12
ST   Mouse STR 2-1: 9
ST   Mouse STR 3-2: 14,15
ST   Mouse STR 4-2: 19.3,20.3
ST   Mouse STR 5-5: 14,15
ST   Mouse STR 6-4: 15.3
ST   Mouse STR 6-7: 12
ST   Mouse STR 7-1: 29
ST   Mouse STR 8-1: 15
ST   Mouse STR 9-2: 15,16
ST   Mouse STR X-1: 25
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
SX   Male
AG   Embryo
CA   Spontaneously immortalized cell line
DT   Created: 04-04-12; Last updated: 30-01-24; Version: 38
//
RX   DOI=10.3917/bhesv.191.0045;
RA   Barreiro E.W., Curras E.;
RT   "Mutation carriers: led by Spaniards in U.S. laboratories in the
RT   early 80s.";
RL   Bull. Hist. Epistem. Sci. Vie 19:45-65(2012).
//
RX   PubMed=2463382; DOI=10.1128/JVI.63.2.753-758.1989;
RA   Raptis L., Bolen J.B.;
RT   "Polyomavirus transforms rat F111 and mouse NIH 3T3 cells by different
RT   mechanisms.";
RL   J. Virol. 63:753-758(1989).
//
RX   PubMed=4087463;
RA   Iwamoto A., Masuda M., Yoshikura H.;
RT   "Two NIH3T3 cell lines of different origins circulating in the
RT   world.";
RL   Jpn. J. Exp. Med. 55:129-131(1985).
//
RX   PubMed=4311790; DOI=10.1128/JVI.4.5.549-553.1969;
RA   Jainchill J.L., Aaronson S.A., Todaro G.J.;
RT   "Murine sarcoma and leukemia viruses: assay using clonal lines of
RT   contact-inhibited mouse cells.";
RL   J. Virol. 4:549-553(1969).
//
RX   PubMed=9753870;
RA   Jayashree B., Visalakshi V., Rajalakshmi K.R., Sukumaran M.S.,
RA   Moni M.S., Rajkumar T., Deshmukh S.;
RT   "Characterization of mouse fibroblast (NIH3T3) and fibrosarcoma cell
RT   lines (WEHI-164 and MFS 8) using PMRS.";
RL   Indian J. Biochem. Biophys. 35:108-114(1998).
//
RX   PubMed=17284666; DOI=10.1152/physiolgenomics.00199.2006;
RA   Menger G.J., Allen G.C., Neuendorff N., Nahm S.-S., Thomas T.L.,
RA   Cassone V.M., Earnest D.J.;
RT   "Circadian profiling of the transcriptome in NIH/3T3 fibroblasts:
RT   comparison with rhythmic gene expression in SCN2.2 cells and the rat
RT   SCN.";
RL   Physiol. Genomics 29:280-289(2007).
//
RX   PubMed=22619183; DOI=10.1002/btpr.1564;
RA   Mancia A., Elliott J.T., Halter M., Bhadriraju K., Tona A.,
RA   Spurlin T.A., Middlebrooks B.L., Baatz J.E., Warr G.W., Plant A.L.;
RT   "Quantitative methods to characterize morphological properties of cell
RT   lines.";
RL   Biotechnol. Prog. 28:1069-1078(2012).
//
RX   PubMed=23321776; DOI=10.1369/0022155413476868;
RA   Leibiger C., Kosyakova N., Mkrtchyan H., Glei M., Trifonov V.A.,
RA   Liehr T.;
RT   "First molecular cytogenetic high resolution characterization of the
RT   NIH 3T3 cell line by murine multicolor banding.";
RL   J. Histochem. Cytochem. 61:306-312(2013).
//
RX   PubMed=23430347; DOI=10.1007/s10616-013-9545-7;
RA   Almeida J.L., Hill C.R., Cole K.D.;
RT   "Mouse cell line authentication.";
RL   Cytotechnology 66:133-147(2014).
//
RX   PubMed=25277546; DOI=10.1186/1471-2164-15-847;
RA   Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W.,
RA   Morse H.C. III, Pardo-Manuel de Villena F.;
RT   "SNP array profiling of mouse cell lines identifies their strains of
RT   origin and reveals cross-contamination and widespread aneuploidy.";
RL   BMC Genomics 15:847.1-847.11(2014).
//
RX   PubMed=31220119; DOI=10.1371/journal.pone.0218412;
RA   Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S.,
RA   Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H.,
RA   Li J.-L., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B.,
RA   Natoli M., Bauer B.A., Gollin S.M., Lewis D.W., Reid Y.A.;
RT   "Interlaboratory study to validate a STR profiling method for
RT   intraspecies identification of mouse cell lines.";
RL   PLoS ONE 14:E0218412-E0218412(2019).
//
RX   PubMed=36078083; DOI=10.3390/cells11172677;
RA   Rahimi A.M., Cai M.-F., Hoyer-Fender S.;
RT   "Heterogeneity of the NIH3T3 fibroblast cell line.";
RL   Cells 11:2677.1-2677.19(2022).
//