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Cellosaurus Flp-In-3T3 (CVCL_U422)

[Text version]
Cell line name Flp-In-3T3
Synonyms Flp-In 3T3; NIH 3T3 Flp-In
Accession CVCL_U422
Resource Identification Initiative To cite this cell line use: Flp-In-3T3 (RRID:CVCL_U422)
Comments Characteristics: Transfected with a pFRT/lacZeo construct that contains a FRT site for subsequent targeted integration of a Flp-In expression vector (Thermo Fisher=R76107).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ).
Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
Cell type: Fibroblast; CL=CL_0000057.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: NIH Swiss.
Hierarchy Parent: CVCL_0594 (NIH 3T3)
Children:
CVCL_E7UG (NIH3T3 Halo-GLI2)CVCL_E7UH (NIH3T3 KIF7-Halo)
Sex of cell Male
Age at sampling Embryo
Category Spontaneously immortalized cell line
Web pages Provider; Thermo Fisher; R76107; https://www.thermofisher.com/order/catalog/product/R76107
Cross-references
Encyclopedic resources Wikidata; Q54835006
Entry history
Entry creation03-Feb-2014
Last entry update10-Apr-2025
Version number11