Cellosaurus cell line NIH3T3 Halo-GLI2 (CVCL

Cellosaurus NIH3T3 Halo-GLI2 (CVCL_E7UG)

[Text version]

Cell line name

NIH3T3 Halo-GLI2

Accession

CVCL_E7UG

Resource Identification Initiative

To cite this cell line use: NIH3T3 Halo-GLI2 (RRID:CVCL_E7UG)

Comments

Characteristics: Transfected with both a pEF5-FRT-DEST-3XHA-Halo-mGLI2 and a pOG44 constructs (PubMed=40073114).
Characteristics: Transfected with a pFRT/lacZeo construct that contains a FRT site for subsequent targeted integration of a Flp-In expression vector (from parent cell line).
Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transfection; Gene=MGI; MGI:95728; Gli2.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ).
Genetic integration: Method=Transfection; Gene=UniProtKB; P0A3G2; Rhodococcus rhodochrous dhaA (Note=Mutated = HaloTag).
Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
Cell type: Fibroblast; CL=CL_0000057.

Species of origin

Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: NIH Swiss.

Hierarchy

Parent: CVCL_U422 (Flp-In-3T3)
Children:

CVCL_E7UJ (NIH3T3 Halo-GLI2+KIF7-Snap)

Sex of cell

Male

Age at sampling

Embryo