Cellosaurus NIH3T3 Halo-GLI2+KIF7-Snap (CVCL_E7UJ)
| Cell line name | NIH3T3 Halo-GLI2+KIF7-Snap |
|---|---|
| Synonyms | NIH3T3 Halo-GLI2 and KIF7-Snap |
| Accession | CVCL_E7UJ |
| Resource Identification Initiative | To cite this cell line use: NIH3T3 Halo-GLI2+KIF7-Snap (RRID:CVCL_E7UJ) |
| Comments | Characteristics: Transduced with a Kif7-SNAP tag construct (PubMed=40073114). Characteristics: Transfected with both a pEF5-FRT-DEST-3XHA-Halo-mGLI2 and a pOG44 constructs (from parent cell line). Characteristics: Transfected with a pFRT/lacZeo construct that contains a FRT site for subsequent targeted integration of a Flp-In expression vector (from parent cell line). Genetic integration: Method=Transduction; Gene=HGNC; HGNC:7059; MGMT (Note=With p.Gly180_Asn207del and 19 mutations = SNAP-tag). Genetic integration: Method=Transduction; Gene=MGI; MGI:1098239; Kif7. Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Genetic integration: Method=Transfection; Gene=MGI; MGI:95728; Gli2. Genetic integration: Method=Transfection; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ). Genetic integration: Method=Transfection; Gene=UniProtKB; P0A3G2; Rhodococcus rhodochrous dhaA (Note=Mutated = HaloTag). Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR). Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922. Cell type: Fibroblast; CL=CL_0000057. |
| Species of origin | Mus musculus (Mouse)
(NCBI Taxonomy: 10090)
Breed/subspecies: NIH Swiss. |
| Hierarchy | Parent: CVCL_E7UG (NIH3T3 Halo-GLI2) |
| Sex of cell | Male |
| Age at sampling | Embryo |
| Category | Spontaneously immortalized cell line |
| Publications | PubMed=40073114; DOI=10.1126/sciadv.adt5439 |
| Entry history | |
| Entry creation | 10-Apr-2025 |
| Last entry update | 10-Apr-2025 |
| Version number | 1 |