ID   NIH3T3 Halo-GLI2+KIF7-Snap
AC   CVCL_E7UJ
SY   NIH3T3 Halo-GLI2 and KIF7-Snap
RX   PubMed=40073114;
CC   Characteristics: Transduced with a Kif7-SNAP tag construct (PubMed=40073114).
CC   Characteristics: Transfected with both a pEF5-FRT-DEST-3XHA-Halo-mGLI2 and a pOG44 constructs (from parent cell line).
CC   Characteristics: Transfected with a pFRT/lacZeo construct that contains a FRT site for subsequent targeted integration of a Flp-In expression vector (from parent cell line).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:7059; MGMT (Note=With p.Gly180_Asn207del and 19 mutations = SNAP-tag).
CC   Genetic integration: Method=Transduction; Gene=MGI; MGI:1098239; Kif7.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Genetic integration: Method=Transfection; Gene=MGI; MGI:95728; Gli2.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P0A3G2; Rhodococcus rhodochrous dhaA (Note=Mutated = HaloTag).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
CC   Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
CC   Cell type: Fibroblast; CL=CL_0000057.
CC   Breed/subspecies: NIH Swiss.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_E7UG ! NIH3T3 Halo-GLI2
SX   Male
AG   Embryo
CA   Spontaneously immortalized cell line
DT   Created: 10-04-25; Last updated: 10-04-25; Version: 1
//
RX   PubMed=40073114; DOI=10.1126/sciadv.adt5439;
RA   Ku P.-I., Sreeja J.S., Chadha A., Williams D.S., Engelke M.F.,
RA   Subramanian R.;
RT   "Collaborative role of two distinct cilium-specific cytoskeletal
rt   systems in driving hedgehog-responsive transcription factor
RT   trafficking.";
RL   Sci. Adv. 11:eadt5439.1-eadt5439.14(2025).
//