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Cellosaurus NEF (CVCL_D900)

[Text version]
Cell line name NEF
Accession CVCL_D900
Resource Identification Initiative To cite this cell line use: NEF (RRID:CVCL_D900)
Comments Characteristics: Expresses ~300000 EGFR (PubMed=1417788).
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:3236; EGFR.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
Cell type: Fibroblast; CL=CL_0000057.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: NIH Swiss.
Hierarchy Parent: CVCL_0594 (NIH 3T3)
Sex of cell Male
Age at sampling Embryo
Category Spontaneously immortalized cell line
Publications

PubMed=2543678; DOI=10.1016/S0021-9258(18)81674-X
Benjamin Lewis Margolis, Irit Lax, Richard Kris, Marker Dombalagian, Annemarie Honegger, Richard Howk, David Givol, Axel Ullrich, Joseph Schlessinger;
All autophosphorylation sites of epidermal growth factor (EGF) receptor and HER2/neu are located in their carboxyl-terminal tails Identification of a novel site in EGF receptor.
J. Biol. Chem. 264:10667-10671(1989)

PubMed=2246335; DOI=10.1002/jcp.1041450223
Nico van Belzen, Marcel Spaargaren, Arie J. Verkleij, Johannes Boonstra;
Interaction of epidermal growth factor receptors with the cytoskeleton is related to receptor clustering.
J. Cell. Physiol. 145:365-375(1990)

PubMed=1417788; DOI=10.1042/bj2870037; PMCID=PMC1133120
Marcel Spaargaren, Sacha Wissink, Libert H.K. Defize, Siegfried Wolfgang de Laat, Johannes Boonstra;
Characterization and identification of an epidermal-growth-factor-activated phospholipase A2.
Biochem. J. 287:37-43(1992)

Cross-references
Anatomy/cell type resources BTO; BTO_0005262
Encyclopedic resources Wikidata; Q54930387
Entry history
Entry creation22-Oct-2012
Last entry update14-Aug-2025
Version number14