Cellosaurus cell line NIH 3T3 Duo Parental (CVCL

Cellosaurus NIH 3T3 Duo Parental (CVCL_HC50)

[Text version]

Cell line name

NIH 3T3 Duo Parental

Accession

CVCL_HC50

Resource Identification Initiative

To cite this cell line use: NIH 3T3 Duo Parental (RRID:CVCL_HC50)

Comments

Characteristics: Cell developed for the induction and visualization of double-strand breaks chromosomal damage at specific sites of the mammalian genome. Sytem based on the stable integration of endonuclease ISceI recognition sites flanked by LacO/TetO operator arrays, coupled with retroviral-mediated integration of their fluorescent repressors (LacR/TetR) to visualize the LacO/TetO sites.
Characteristics: Integrates one copy of a LacO-ISceI construct (256 copies of LacO) and two copies of a TetO-ISceI-TetO construct (96 copies of TetO).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P03023; Escherichia coli lacI.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
Cell type: Fibroblast; CL=CL_0000057.

Species of origin

Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: NIH Swiss.

Hierarchy

Parent: CVCL_0594 (NIH 3T3)
Children:

CVCL_HC51 (NIH 3T3 Duo)

Sex of cell

Male

Age at sampling

Embryo