Cellosaurus cell line NIH 3T3 Duo (CVCL

Cellosaurus NIH 3T3 Duo (CVCL_HC51)

[Text version]

Cell line name

NIH 3T3 Duo

Synonyms

NIH3T3duo

Accession

CVCL_HC51

Resource Identification Initiative

To cite this cell line use: NIH 3T3 Duo (RRID:CVCL_HC51)

Comments

Characteristics: Cell developed for the induction and visualization of double-strand breaks chromosomal damage at specific sites of the mammalian genome. Sytem based on the stable integration of endonuclease ISceI recognition sites flanked by LacO/TetO operator arrays, coupled with retroviral-mediated integration of their fluorescent repressors (LacR/TetR) to visualize the LacO/TetO sites.
Characteristics: Integrates one copy of a LacO-ISceI construct (256 copies of LacO), two copies of a TetO-ISceI-TetO construct (96 copies of TetO) as well as a LacR-GFP and a TetR-mCherry constructs.
Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transfection/transduction; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P03023; Escherichia coli lacI.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
Cell type: Fibroblast; CL=CL_0000057.

Species of origin

Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: NIH Swiss.

Hierarchy

Parent: CVCL_HC50 (NIH 3T3 Duo Parental)
Children:

CVCL_HC52 (NIH 3T3 Duo-BFP53BP1)

Sex of cell

Male

Age at sampling

Embryo