Cellosaurus NIH 3T3 Duo-BFP53BP1 (CVCL_HC52)
| Cell line name | NIH 3T3 Duo-BFP53BP1 |
|---|---|
| Accession | CVCL_HC52 |
| Resource Identification Initiative | To cite this cell line use: NIH 3T3 Duo-BFP53BP1 (RRID:CVCL_HC52) |
| Comments | Characteristics: Cell developed for the induction and visualization of double-strand breaks chromosomal damage at specific sites of the mammalian genome. Sytem based on the stable integration of endonuclease ISceI recognition sites flanked by LacO/TetO operator arrays, coupled with retroviral-mediated integration of their fluorescent repressors (LacR/TetR) to visualize the LacO/TetO sites. Characteristics: Integrates one copy of a LacO-ISceI construct (256 copies of LacO), two copies of a TetO-ISceI-TetO construct (96 copies of TetO) as well as a LacR-GFP and a TetR-mCherry constructs. Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transfection/transduction; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)). Genetic integration: Method=Transfection/transduction; Gene=FPbase; BFJKS; TagBFP (Note=Blue fluorescent protein derived from eqFP578). Genetic integration: Method=Transfection/transduction; Gene=MGI; MGI:1351320; Trp53bp1. Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P03023; Escherichia coli lacI. Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR. Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922. Cell type: Fibroblast; CL=CL_0000057. |
| Species of origin | Mus musculus (Mouse)
(NCBI Taxonomy: 10090)
Breed/subspecies: NIH Swiss. |
| Hierarchy | Parent: CVCL_HC51 (NIH 3T3 Duo) |
| Sex of cell | Male |
| Age at sampling | Embryo |
| Category | Spontaneously immortalized cell line |
| Publications | PubMed=23929981; DOI=10.1126/science.1237150; PMCID=PMC6324928 |
| Cross-references | |
| Cell line collections (Providers) | Kerafast; ENH104-FP |
| Encyclopedic resources | Wikidata; Q54930577 |
| Entry history | |
| Entry creation | 01-Dec-2016 |
| Last entry update | 10-Apr-2025 |
| Version number | 14 |