ID   NIH 3T3 Duo-BFP53BP1
AC   CVCL_HC52
DR   Kerafast; ENH104-FP
DR   Wikidata; Q54930577
RX   PubMed=23929981;
CC   Characteristics: Cell developed for the induction and visualization of double-strand breaks chromosomal damage at specific sites of the mammalian genome. Sytem based on the stable integration of endonuclease ISceI recognition sites flanked by LacO/TetO operator arrays, coupled with retroviral-mediated integration of their fluorescent repressors (LacR/TetR) to visualize the LacO/TetO sites.
CC   Characteristics: Integrates one copy of a LacO-ISceI construct (256 copies of LacO), two copies of a TetO-ISceI-TetO construct (96 copies of TetO) as well as a LacR-GFP and a TetR-mCherry constructs.
CC   Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transfection/transduction; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
CC   Genetic integration: Method=Transfection/transduction; Gene=FPbase; BFJKS; TagBFP (Note=Blue fluorescent protein derived from eqFP578).
CC   Genetic integration: Method=Transfection/transduction; Gene=MGI; MGI:1351320; Trp53bp1.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P03023; Escherichia coli lacI.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
CC   Cell type: Fibroblast; CL=CL_0000057.
CC   Breed/subspecies: NIH Swiss.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_HC51 ! NIH 3T3 Duo
SX   Male
AG   Embryo
CA   Spontaneously immortalized cell line
DT   Created: 01-12-16; Last updated: 10-04-25; Version: 14
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RX   PubMed=23929981; DOI=10.1126/science.1237150; PMCID=PMC6324928;
RA   Roukos V., Voss T.C., Schmidt C.K., Lee S., Wangsa D., Misteli T.;
RT   "Spatial dynamics of chromosome translocations in living cells.";
RL   Science 341:660-664(2013).
//