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Cellosaurus Huh-7 (CVCL_0336)

[Text version]
Cell line name Huh-7
Synonyms HuH-7; HUH-7; HuH7; Huh7; HUH7; HUH7.0; JTC-39; Japanese Tissue Culture-39
Accession CVCL_0336
Resource Identification Initiative To cite this cell line use: Huh-7 (RRID:CVCL_0336)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: ENCODE project common cell types; tier 3.
Part of: JFCR45 cancer cell line panel.
Part of: Liver Cancer Model Repository (LIMORE).
Part of: MD Anderson Cell Lines Project.
Part of: TCGA-110-CL cell line panel.
Population: Japanese.
Doubling time: 22 +- 1 hours (PubMed=11152498); 32.2 hours (PubMed=31378681); ~48 hours (CLS=300156).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Genomics; Whole genome sequencing.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; Expression; Reverse-phase protein array.
Omics: Proteomics; Quantitative.
Omics: Proteomics; Subcellular; Mitochondrion.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; miRNA profiling; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Sequence variations
HLA typing Source: PubMed=26589293
Class I
HLA-AA*11:01,11:01
HLA-BB*08:03,35:09
HLA-CC*01:02,01:02

Source: CLS=300156
Class I
HLA-AA*11:01:01
HLA-BB*54:01:01
HLA-CC*01:02:01
Class II
HLA-DPDPB1*02:01:02
HLA-DQDQA1*01:03:01
DQB1*06:01:01
HLA-DRDRB1*08:03:02
Genome ancestry Source: PubMed=30894373

Origin% genome
African1.56
Native American0
East Asian, North79.68
East Asian, South13.08
South Asian0
European, North0
European, South5.69
Disease Adult hepatocellular carcinoma (NCIt: C7956)
Adult hepatocellular carcinoma (ORDO: Orphanet_210159)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_U442 (B76/Huh7)CVCL_RW46 (HG23)CVCL_X943 (Huh-5-2)
CVCL_C8QS (Huh-7 clone 5-15)CVCL_C8QU (Huh-7 clone 8-1)CVCL_C8QT (Huh-7 clone 9-13)
CVCL_C8ZK (HUH-7 SR)CVCL_C8W2 (HuH-7-EGFP)CVCL_ZW90 (HuH-7-END)
CVCL_JG51 (Huh-7-Luc [JCRB])CVCL_C8W3 (HuH-7-Luc [Ubigene])CVCL_U459 (Huh-7-Lunet)
CVCL_7927 (Huh-7.5)CVCL_WJ09 (Huh-7/F24)CVCL_XE85 (Huh-7/M8)
CVCL_2957 (Huh-7D12)CVCL_LG22 (HuH-7T1)CVCL_A5AV (Huh7.93)
CVCL_UZ56 (Huh7 IFITM2-/-)CVCL_C8HQ (HuH7-2C8)CVCL_D4RG (HuH7-FLVCR2-KO-c1)
CVCL_D4RH (HuH7-FLVCR2-KO-c5)CVCL_HA62 (HUH7-ins)CVCL_D4RI (HuH7-MPC2-KO-c10)
CVCL_D4RJ (HuH7-MPC2-KO-c12)CVCL_D4RK (HuH7-NPC1L1-KO-c6)CVCL_B7TI (HUH7-R)
CVCL_D4RL (HuH7-SFXN5-KO-c12)CVCL_D4RM (HuH7-SFXN5-KO-c14)CVCL_D4RN (HuH7-SLC12A5-KO-c3)
CVCL_D4RP (HuH7-SLC12A5-KO-c4)CVCL_D4RQ (HuH7-SLC13A5-KO-c4)CVCL_D4RR (HuH7-SLC13A5-KO-c5)
CVCL_D4RS (HuH7-SLC15A1-KO-c2)CVCL_D4RT (HuH7-SLC15A1-KO-c6)CVCL_D4RU (HuH7-SLC16A12-KO-c1)
CVCL_D4RV (HuH7-SLC16A12-KO-c9)CVCL_D4RW (HuH7-SLC17A1-KO-c1)CVCL_D4RX (HuH7-SLC17A1-KO-c3)
CVCL_D4RY (HuH7-SLC17A2-KO-c2)CVCL_D4RZ (HuH7-SLC17A2-KO-c3)CVCL_D4S0 (HuH7-SLC17A8-KO-c1)
CVCL_D4S1 (HuH7-SLC17A8-KO-c4)CVCL_D4S2 (HuH7-SLC17A9-KO-c11)CVCL_D4S3 (HuH7-SLC17A9-KO-c16)
CVCL_D4S4 (HuH7-SLC19A3-KO-c2)CVCL_D4S5 (HuH7-SLC19A3-KO-c6)CVCL_D4S6 (HuH7-SLC22A10-KO-c10)
CVCL_D4S7 (HuH7-SLC22A10-KO-c15)CVCL_D4S8 (HuH7-SLC22A15-KO-c7)CVCL_D4S9 (HuH7-SLC22A15-KO-c8)
CVCL_D4SA (HuH7-SLC22A3-KO-c14)CVCL_D4SB (HuH7-SLC22A3-KO-c4)CVCL_D4SC (HuH7-SLC22A7-KO-c2)
CVCL_D4SD (HuH7-SLC22A7-KO-c6)CVCL_D4SE (HuH7-SLC22A9-KO-c4)CVCL_D4SF (HuH7-SLC22A9-KO-c5)
CVCL_D4SG (HuH7-SLC23A1-KO-c2)CVCL_D4SH (HuH7-SLC23A1-KO-c3)CVCL_D4SI (HuH7-SLC25A18-KO-c10)
CVCL_D4SJ (HuH7-SLC25A18-KO-c3)CVCL_D4SK (HuH7-SLC25A18-KO-c9)CVCL_D4SL (HuH7-SLC25A20-KO-c1)
CVCL_D4SM (HuH7-SLC25A20-KO-c6)CVCL_D4SN (HuH7-SLC25A42-KO-c1)CVCL_D4SP (HuH7-SLC25A42-KO-c2)
CVCL_D4SQ (HuH7-SLC25A45-KO-c3)CVCL_D4SR (HuH7-SLC25A45-KO-c6)CVCL_D4SS (HuH7-SLC26A1-KO-c1)
CVCL_D4ST (HuH7-SLC26A1-KO-c2)CVCL_D4SU (HuH7-SLC26A3-KO-c3)CVCL_D4SV (HuH7-SLC26A3-KO-c6)
CVCL_D4SW (HuH7-SLC29A3-KO-c12)CVCL_D4SX (HuH7-SLC29A3-KO-c13)CVCL_D4SY (HuH7-SLC2A14-KO-c2)
CVCL_D4SZ (HuH7-SLC2A14-KO-c4)CVCL_D4T0 (HuH7-SLC2A2-KO-c5)CVCL_D4T1 (HuH7-SLC2A2-KO-c7)
CVCL_D4T2 (HuH7-SLC2A4-KO-c1)CVCL_D4T3 (HuH7-SLC2A4-KO-c2)CVCL_D4T4 (HuH7-SLC2A8-KO-c5)
CVCL_D4T5 (HuH7-SLC2A8-KO-c6)CVCL_D4T6 (HuH7-SLC30A10-KO-c18)CVCL_D4T7 (HuH7-SLC30A10-KO-c5)
CVCL_D4T8 (HuH7-SLC35D1-KO-c2)CVCL_D4T9 (HuH7-SLC35D1-KO-c9)CVCL_D4TA (HuH7-SLC35D2-KO-c3)
CVCL_D4TB (HuH7-SLC35D2-KO-c4)CVCL_D4TC (HuH7-SLC37A4-KO-c5)CVCL_D4TD (HuH7-SLC37A4-KO-c6)
CVCL_D4TE (HuH7-SLC38A11-KO-c4)CVCL_D4TF (HuH7-SLC38A3-KO-c2)CVCL_D4TG (HuH7-SLC38A3-KO-c3)
CVCL_D4TH (HuH7-SLC38A4-KO-c1)CVCL_D4TI (HuH7-SLC38A4-KO-c2)CVCL_D4TJ (HuH7-SLC38A6-KO-c1)
CVCL_D4TK (HuH7-SLC38A6-KO-c12)CVCL_D4TL (HuH7-SLC38A6-KO-c14)CVCL_D4TM (HuH7-SLC3A1-KO-c1)
CVCL_D4TN (HuH7-SLC3A1-KO-c2)CVCL_D4TP (HuH7-SLC40A1-KO-c3)CVCL_D4TQ (HuH7-SLC40A1-KO-c5)
CVCL_D4TR (HuH7-SLC43A1-KO-c1)CVCL_D4TS (HuH7-SLC43A1-KO-c12)CVCL_D4TT (HuH7-SLC44A5-KO-c3)
CVCL_D4TU (HuH7-SLC44A5-KO-c5)CVCL_D4TV (HuH7-SLC47A1-KO-c4)CVCL_D4TW (HuH7-SLC47A1-KO-c7)
CVCL_D4TX (HuH7-SLC4A4-KO-c2)CVCL_D4TY (HuH7-SLC4A4-KO-c4)CVCL_D4TZ (HuH7-SLC51A-KO-c1)
CVCL_D4U0 (HuH7-SLC51A-KO-c2)CVCL_D4U1 (HuH7-SLC51B-KO-c10)CVCL_D4U2 (HuH7-SLC51B-KO-c2)
CVCL_D4U3 (HuH7-SLC5A12-KO-c1)CVCL_D4U4 (HuH7-SLC5A12-KO-c2)CVCL_D4U5 (HuH7-SLC5A8-KO-c2)
CVCL_D4U6 (HuH7-SLC5A8-KO-c3)CVCL_D4U7 (HuH7-SLC5A9-KO-c13)CVCL_D4U8 (HuH7-SLC5A9-KO-c7)
CVCL_D4U9 (HuH7-SLC6A11-KO-c2)CVCL_D4UA (HuH7-SLC6A11-KO-c5)CVCL_D4UB (HuH7-SLC6A12-KO-c3)
CVCL_D4UC (HuH7-SLC6A12-KO-c8)CVCL_D4UD (HuH7-SLC6A19-KO-c10)CVCL_D4UE (HuH7-SLC6A19-KO-c7)
CVCL_D4UF (HuH7-SLC6A4-KO-c2)CVCL_D4UG (HuH7-SLC6A4-KO-c3)CVCL_D4UH (HuH7-SLC7A10-KO-c6)
CVCL_D4UI (HuH7-SLC7A10-KO-c7)CVCL_D4UJ (HuH7-SLC7A2-KO-c4)CVCL_D4UK (HuH7-SLC7A2-KO-c5)
CVCL_D4UL (HuH7-SLC7A7-KO-c6)CVCL_D4UM (HuH7-SLC7A7-KO-c9)CVCL_D4UN (HuH7-SLCO1A2-KO-c4)
CVCL_D4UP (HuH7-SLCO1A2-KO-c5)CVCL_D4UQ (HuH7-SLCO2A1-KO-c2)CVCL_D4UR (HuH7-SLCO2A1-KO-c3)
CVCL_D4US (HuH7-SLCO2B1-KO-c2)CVCL_D4UT (HuH7-SLCO2B1-KO-c3)CVCL_D4UU (HuH7-SLCO4C1-KO-c1)
CVCL_D4UV (HuH7-SLCO4C1-KO-c7)CVCL_D4UW (HuH7-SVOPL-KO-c2)CVCL_D4UX (HuH7-SVOPL-KO-c3)
CVCL_D4UY (HuH7-UCP1-KO-c11)CVCL_D4UZ (HuH7-UCP1-KO-c9)CVCL_D4V0 (HuH7-UNC93A-KO-c11)
CVCL_D4V1 (HuH7-UNC93A-KO-c3)CVCL_4W53 (Huh7S1)CVCL_D6BU (HyCyte Huh-7 KO-hSLC39A1)
CVCL_E1LF (HyCyte Huh-7 KO-hTIMM17B)CVCL_E3HL (HyCyte Huh-7-Luc)CVCL_VN30 (OR6 [Human])
CVCL_E0VE (Ubigene Huh-7 ALDH5A1 KO)CVCL_E0VF (Ubigene Huh-7 ATG5 KO)CVCL_E0VG (Ubigene Huh-7 BCAT1 KO)
CVCL_E0VH (Ubigene Huh-7 DCP1A KO)CVCL_E0VI (Ubigene Huh-7 DCP1B KO)CVCL_E0VJ (Ubigene Huh-7 DKKL1 KO)
CVCL_E0VK (Ubigene Huh-7 EIF4ENIF1 KO)CVCL_E0VL (Ubigene Huh-7 FAM216A KO)CVCL_E0VM (Ubigene Huh-7 FBXO28 KO)
CVCL_E0VN (Ubigene Huh-7 GSDME KO)CVCL_E0VP (Ubigene Huh-7 HMGB2 KO)CVCL_E0VQ (Ubigene Huh-7 LDHA KO)
CVCL_E0VR (Ubigene Huh-7 LDHB KO)CVCL_E0VS (Ubigene Huh-7 LEAP2 KO)CVCL_E0VT (Ubigene Huh-7 NOTCH3 KO)
CVCL_E0VU (Ubigene Huh-7 NR1H4 KO)CVCL_E0VV (Ubigene Huh-7 NUP133 KO)CVCL_E0VW (Ubigene Huh-7 PFKFB3 KO)
CVCL_E0VX (Ubigene Huh-7 PNPLA3 KO)CVCL_E0VY (Ubigene Huh-7 SCUBE3 KO)CVCL_E0VZ (Ubigene Huh-7 TREM1 KO)
Sex of cell Male
Age at sampling 57Y
Category Cancer cell line
STR profile Source(s): CCRID; CLS=300156; Cosmic-CLP=907071; JCRB=JCRB0403; KCLB=60104; PubMed=25877200; PubMed=29774518; PubMed=31378681; RCB=RCB1366; Technion Genomics Center; TKG=TKG 0206

Markers:
AmelogeninX
CSF1PO11
D1S165616
D2S44112,14
D2S133819
D3S135815
D5S81812
D6S104313,15
D7S82011
D8S117914,15
D10S124816
D12S39120 (CLS=300156; Technion Genomics Center)
20,21 (CCRID)
D13S31710 (CLS=300156; RCB=RCB1366; Technion Genomics Center)
10,11 (CCRID; Cosmic-CLP=907071; JCRB=JCRB0403; KCLB=60104; PubMed=25877200; PubMed=29774518; PubMed=31378681; TKG=TKG 0206)
D16S53910
D18S5115
D19S43313,14
D21S1130
D22S104517,20
FGA22,23
Penta D12
Penta E11
TH017
TPOX8,11
vWA16,18 (CCRID; Cosmic-CLP=907071; JCRB=JCRB0403; KCLB=60104; PubMed=25877200; PubMed=29774518; PubMed=31378681; RCB=RCB1366; TKG=TKG 0206)
18 (CLS=300156; Technion Genomics Center)

Run an STR similarity search on this cell line
Web pages Info; -; -; https://huh7.com/
Info; ENCODE; -; http://genome.ucsc.edu/ENCODE/protocols/cell/human/Huh-7_Crawford_protocol.pdf
Info; LCCL; -; https://lccl.zucmanlab.com/hcc/cellLines/Huh7
Info; MCLP; -; https://tcpaportal.org/mclp/
Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/h/cell-lines-detail-309.html
Info; Wikipedia; -; https://en.wikipedia.org/wiki/Huh7
Provider; Altogen; Xenograft model; https://altogenlabs.com/xenograft-models/liver-cancer-xenograft/huh7-xenograft-model/
Provider; BRICS; BRICS0114; http://www.brics.ac.cn/cell/template/cell/cell_detail.html?id=3919
Publications

PubMed=6203805; DOI=10.20772/cancersci1959.75.2_151
Hidekazu Nakabayashi, Kazuhisa Taketa, Takashi Yamane, Masahiro Miyazaki, Keiko Miyano, Jiro Sato;
Phenotypical stability of a human hepatoma cell line, HuH-7, in long-term culture with chemically defined medium.
Gann 75:151-158(1984)

DOI=10.1007/978-4-431-68349-0_4
Jennifer J. Alexander;
Human hepatoma cell lines.
(In book chapter) Neoplasms of the liver; Okuda, Kunio & Ishak, Kamal George (eds.); pp.47-56; Springer; Tokyo; Japan (1987)

DOI=10.11418/jtca1981.12.3_221
Masayoshi Namba, Hidekazu Nakabayashi, Isao Doi, Jiro Sato, Masahiro Miyazaki;
Cellular characteristics and utilization of human hepatoma cell lines which were established in our laboratory and distributed by Japanese Cancer Research Resources Bank.
Tissue Cult. Res. Commun. 12:221-227(1993)

PubMed=8224613; DOI=10.1096/fasebj.7.14.8224613
Alain Puisieux, Katherine Galvin, Frederic Troalen, Brigitte Bressac, Christophe Marcais, Eithan Galun, Frederique Ponchel, Cengiz Yakicier, Jing-Wei Ji, Mehmet Ozturk;
Retinoblastoma and p53 tumor suppressor genes in human hepatoma cell lines.
FASEB J. 7:1407-1413(1993)

PubMed=8389256; DOI=10.1093/carcin/14.5.987
Ih-Chang Hsu, Takayoshi Tokiwa, William P. Bennett, Robert A. Metcalf, Judith A. Welsh, Tsung-Tang Sun, Curtis Craig Harris;
p53 gene mutation and integrated hepatitis B viral DNA sequences in human liver cancer cell lines.
Carcinogenesis 14:987-992(1993)

PubMed=8835345; DOI=10.1002/(SICI)1096-9071(199602)48:2<133::AID-JMV3>3.0.CO;2-A
So Tsuboi, Seishi Nagamori, Masahiro Miyazaki, Koichiro Mihara, Ken-ichi Fukaya, Kiichiro Teruya, Tsunenori Kosaka, Takao Tsuji, Masayoshi Namba;
Persistence of hepatitis C virus RNA in established human hepatocellular carcinoma cell lines.
J. Med. Virol. 48:133-140(1996)

DOI=10.11418/jtca1981.16.3_173
Koichiro Mihara, Masahiro Miyazaki, Kazuo Fushimi, Toshiya Tsuji, Yusuke Inoue, Ken-ichi Fukaya, Ryuichiro Ohashi, Masayoshi Namba;
The p53 gene status and other cellular characteristics of human cell lines maintained in our laboratory.
Tissue Cult. Res. Commun. 16:173-178(1997)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Li-Qun Jia, Motonobu Osada, Chikashi Ishioka, Makio Gamo, Shuntaro Ikawa, Takao Suzuki, Hideki Shimodaira, Tomohito Niitani, Toshio Kudo, Mitoshi Akiyama, Narimiti Kimura ...Show all 18 authors... , Mitsuyoshi Matsuo, Hiroshi Mizusawa, Noriho Tanaka, Hideki Koyama, Masayoshi Namba, Ryunosuke Kanamaru, Toshio Kuroki; Show fewer authors
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

PubMed=9359923; DOI=10.18926/AMO/30789
Koichiro Mihara, Masahiro Miyazaki, Tadashi Kondo, Kazuo Fushimi, Toshiya Tsuji, Yusuke Inoue, Ken-ichi Fukaya, Chikashi Ishioka, Masayoshi Namba;
Yeast functional assay of the p53 gene status in human cell lines maintained in our laboratory.
Acta Med. Okayama 51:261-265(1997)

PubMed=10523694; DOI=10.3892/or.6.6.1267
Chong Gao, Ryuichiro Ohashi, Hong Pu, Yusuke Inoue, Toshiya Tsuji, Masahiro Miyazaki, Masayoshi Namba;
Yeast functional assay of the p53 gene status in 11 cell lines and 26 surgical specimens of human hepatocellular carcinoma.
Oncol. Rep. 6:1267-1271(1999)

PubMed=11152498; DOI=10.1128/JVI.75.3.1252-1264.2001; PMCID=PMC114031
Thomas Pietschmann, Volker Lohmann, Gabriel Rutter, Katharina Kurpanek, Ralf Friedrich Wilhelm Bartenschlager;
Characterization of cell lines carrying self-replicating hepatitis C virus RNAs.
J. Virol. 75:1252-1264(2001)

PubMed=12029633; DOI=10.1053/jhep.2002.33683
Kohichiroh Yasui, Shigeki Arii, Chen Zhao, Issei Imoto, Masakazu Ueda, Hisaki Nagai, Mitsuru Emi, Johji Inazawa;
TFDP1, CUL4A, and CDC16 identified as targets for amplification at 13q34 in hepatocellular carcinomas.
Hepatology 35:1476-1484(2002)

PubMed=15708988; DOI=10.1128/JVI.79.5.2689-2699.2005; PMCID=PMC548482
Rhea Myers Sumpter Jr., Yueh-Ming Loo, Eileen Marie Foy, Kui Li, Mitsutoshi Yoneyama, Takashi Fujita, Stanley M. Lemon, Michael Gale Jr.;
Regulating intracellular antiviral defense and permissiveness to hepatitis C virus RNA replication through a cellular RNA helicase, RIG-I.
J. Virol. 79:2689-2699(2005)

PubMed=15767549; DOI=10.1158/1535-7163.MCT-04-0234
Noriyuki Nakatsu, Yoko Yoshida, Kanami Yamazaki, Tomoki Nakamura, Shingo Dan, Yasuhisa Fukui, Takao Yamori;
Chemosensitivity profile of cancer cell lines and identification of genes determining chemosensitivity by an integrated bioinformatical approach using cDNA arrays.
Mol. Cancer Ther. 4:399-412(2005)

PubMed=16935386; DOI=10.1016/j.jhep.2006.05.019
Dian-Xing Sun, Michael Nassal;
Stable HepG2- and Huh7-based human hepatoma cell lines for efficient regulated expression of infectious hepatitis B virus.
J. Hepatol. 45:636-645(2006)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
S. Michael Rothenberg, Gayatry Mohapatra, Miguel N. Rivera, Daniel Winokur, Patricia Greninger, Mai Nitta, Peter M. Sadow, Gaya Sooriyakumar, Brian W. Brannigan, Matthew J. Ulman, Rushika M. Perera ...Show all 22 authors... , Rui Wang, Angela Tam, Xiao-Jun Ma, Mark Erlander, Dennis C. Sgroi, James W. Rocco, Mark W. Lingen, Ezra E.W. Cohen, David Neil Louis, Jeffrey Settleman, Daniel Arie Haber; Show fewer authors
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Jordi Ginesta Barretina, Giordano Caponigro, Nicolas Stransky, Kavitha Venkatesan, Adam A. Margolin, Sungjoon Kim, Christopher J. Wilson, Joseph Lehar, Gregory V. Kryukov, Dmitriy Sonkin, Anupama Reddy ...Show all 61 authors... , Manway Liu, Lauren Murray, Michael F. Berger, John E. Monahan, Paula Morais, Jodi Meltzer, Adam Korejwa, Judit Jane-Valbuena, Felipa A. Mapa, Joseph Thibault, Eva Bric-Furlong, Pichai Raman, Aaron Shipway, Ingo H. Engels, Jill Cheng, Guo-Ying K. Yu, Jian-Jun Yu, Peter Aspesi Jr., Melanie de Silva, Kalpana Jagtap, Michael D. Jones, Li Wang, Charles Hatton, Emanuele Palescandolo, Supriya Gupta, Scott Mahan, Carrie Sougnez, Robert C. Onofrio, Ted Liefeld, Laura E. MacConaill, Wendy Winckler, Michael Reich, Nan-Xin Li, Jill P. Mesirov, Stacey B. Gabriel, Gad Getz, Kristin Ardlie, Vivien Chan, Vic E. Myer, Barbara L. Weber, Jeff Porter, Markus Warmuth, Peter Finan, Jennifer L. Harris, Matthew Langer Meyerson, Todd Robert Golub, Michael P. Morrissey, William Raj Sellers, Robert Schlegel, Levi Alexander Garraway; Show fewer authors
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=23285155; DOI=10.1371/journal.pone.0052697; PMCID=PMC3527576
Asako Murayama, Nao Sugiyama, Seiko Yoshimura, Mitsuko Ishihara-Sugano, Takahiro Masaki, Sulyi Kim, Takaji Wakita, Shunji Mishiro, Takanobu Kato;
A subclone of HuH-7 with enhanced intracellular hepatitis C virus production and evasion of virus related-cell cycle arrest.
PLoS ONE 7:e52697.1-e52697.9(2012)

PubMed=23505090; DOI=10.1002/hep.26402
Kai Wang, Ho Yeong Lim, Stephanie Shi, Jeeyun Lee, Shi-Bing Deng, Tao Xie, Zhou Zhu, Yu-Li Wang, David Pocalyko, Wei Jennifer Yang, Paul A. Rejto ...Show all 14 authors... , Mao Mao, Cheol-Keun Park, Jiang-Chun Xu; Show fewer authors
Genomic landscape of copy number aberrations enables the identification of oncogenic drivers in hepatocellular carcinoma.
Hepatology 58:706-717(2013)

PubMed=23887712; DOI=10.1038/ncomms3218; PMCID=PMC3731665
Jean-Charles Nault, Maxime Mallet, Camilla Pilati, Julien Calderaro, Paulette Bioulac-Sage, Christophe Laurent, Alexis Laurent, Daniel Cherqui, Charles Balabaud, Jessica Zucman-Rossi;
High frequency of telomerase reverse-transcriptase promoter somatic mutations in hepatocellular carcinoma and preneoplastic lesions.
Nat. Commun. 4:2218.1-2218.7(2013)

PubMed=24973239; DOI=10.1099/vir.0.065995-0
Maria Richter, Ilona Reimann, Horst Schirrmeier, Peter D. Kirkland, Martin Beer;
The viral envelope is not sufficient to transfer the unique broad cell tropism of Bungowannah virus to a related pestivirus.
J. Gen. Virol. 95:2216-2222(2014)

PubMed=25485619; DOI=10.1038/nbt.3080
Christiaan Klijn, Steffen Durinck, Eric W. Stawiski, Peter M. Haverty, Zhao-Shi Jiang, Han-Bin Liu, Jeremiah Degenhardt, Oleg Mayba, Florian Gnad, Jin-Feng Liu, Gregoire Pau ...Show all 30 authors... , Jens Reeder, Yi Cao, Kiran Mukhyala, Suresh K. Selvaraj, Ma-Mie Yu, Gregory J. Zynda, Matthew J. Brauer, Thomas D. Wu, Robert Clifford Gentleman, Gerard Manning, Robert L. Yauch, Richard Bourgon, David Stokoe, Zora Modrusan, Richard M. Neve, Frederic J. de Sauvage, Jeffrey Settleman, Somasekar Seshagiri, Ze-Min Zhang; Show fewer authors
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25574106; DOI=10.3748/wjg.v21.i1.311; PMCID=PMC4284350
Dilek Cevik, Gokhan Yildiz, Mehmet Ozturk;
Common telomerase reverse transcriptase promoter mutations in hepatocellular carcinomas from different geographical locations.
World J. Gastroenterol. 21:311-317(2015)

PubMed=25877200; DOI=10.1038/nature14397
Ma-Mie Yu, Suresh K. Selvaraj, May M.Y. Liang-Chu, Sahar Aghajani, Matthew Busse, Jean Yuan, Genee Lee, Franklin V. Peale, Christiaan Klijn, Richard Bourgon, Joshua S. Kaminker, Richard M. Neve;
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Jelle Scholtalbers, Sebastian Boegel, Thomas Bukur, Marius Byl, Sebastian Goerges, Patrick Sorn, Martin Loewer, Ugur Sahin, John C. Castle;
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=27329724; DOI=10.18632/oncotarget.10161; PMCID=PMC5216950
Kosuke Watari, Ayumi Nishitani, Tomohiro Shibata, Masaki Noda, Akihiko Kawahara, Jun Akiba, Yuichi Murakami, Hirohisa Yano, Michihiko Kuwano, Mayumi Ono;
Phosphorylation of mTOR Ser2481 is a key target limiting the efficacy of rapalogs for treating hepatocellular carcinoma.
Oncotarget 7:47403-47417(2016)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005; PMCID=PMC5501076
Jun Li, Wei Zhao, Rehan Akbani, Wen-Bin Liu, Zhen-Lin Ju, Shi-Yun Ling, Christopher P. Vellano, Paul Roebuck, Qing-Hua Yu, A. Karina Eterovic, Lauren Averett Byers ...Show all 25 authors... , Michael A. Davies, Wan-Leng Deng, Y.N. Vashisht Gopal, Guo Chen, Erika Maria von Euw, Dennis Joseph Slamon, Dylan Conklin, John Victor Heymach, Adi F. Gazdar, John D. Minna, Jeffrey N. Myers, Yi-Ling Lu, Gordon B. Mills, Han Liang; Show fewer authors
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

PubMed=29610054; DOI=10.1016/j.dmpk.2018.03.003; PMCID=PMC6309175
Jian Shi, Xin-Wen Wang, Ling-Yun Lyu, Hui Jiang, Hao-Jie Zhu;
Comparison of protein expression between human livers and the hepatic cell lines HepG2, Hep3B, and Huh7 using SWATH and MRM-HR proteomics: Focusing on drug-metabolizing enzymes.
Drug Metab. Pharmacokinet. 33:133-140(2018)

PubMed=29774518; DOI=10.1007/s13577-018-0212-3; PMCID=PMC6002425
Fumio Kasai, Noriko Hirayama, Midori Ozawa, Motonobu Satoh, Arihiro Kohara;
HuH-7 reference genome profile: complex karyotype composed of massive loss of heterozygosity.
Hum. Cell 31:261-267(2018)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31063779; DOI=10.1053/j.gastro.2019.05.001
Stefano Caruso, Anna-Line Calatayud, Jill Pilet, Tiziana La Bella, Samia Rekik, Sandrine Imbeaud, Eric Letouze, Lea Meunier, Quentin Bayard, Nataliya Rohr-Udilova, Camille Peneau ...Show all 20 authors... , Bettina Grasl-Kraupp, Leanne de Koning, Berengere Ouine, Paulette Bioulac-Sage, Gabrielle Couchy, Julien Calderaro, Jean-Charles Nault, Jessica Zucman-Rossi, Sandra Rebouissou; Show fewer authors
Analysis of liver cancer cell lines identifies agents with likely efficacy against hepatocellular carcinoma and markers of response.
Gastroenterology 157:760-776(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31378681; DOI=10.1016/j.ccell.2019.07.001; PMCID=PMC7505724
Zhi-Xin Qiu, Hong Li, Zheng-Tao Zhang, Zhen-Feng Zhu, Sheng He, Xu-Jun Wang, Peng-Cheng Wang, Jian-Jie Qin, Li-Ping Zhuang, Wei Wang, Fu-Bo Xie ...Show all 33 authors... , Ying Gu, Ke-Ke Zou, Chao Li, Chun Li, Chen-Hua Wang, Jin Cen, Xiao-Tao Chen, Ya-Jing Shu, Zhao Zhang, Lu-Lu Sun, Li-Hua Min, Yong Fu, Xiao-Wu Huang, Hui Lv, He Zhou, Yuan Ji, Zhi-Gang Zhang, Zhi-Qiang Meng, Xiao-Lei Shi, Hai-Bin Zhang, Yi-Xue Li, Li-Jian Hui; Show fewer authors
A pharmacogenomic landscape in human liver cancers.
Cancer Cell 36:179-193.e11(2019)

PubMed=31395879; DOI=10.1038/s41467-019-11415-2; PMCID=PMC6687785
Katharine Yu, Bin Chen, Dvir Aran, Joe Charalel, Christina Yau, Douglas M. Wolf, Laura J. van 't Veer, Atul Janardhan Butte, Ted Goldstein, Marina Sirota;
Comprehensive transcriptomic analysis of cell lines as models of primary tumors across 22 tumor types.
Nat. Commun. 10:3574.1-3574.11(2019)

PubMed=31903165; DOI=10.18632/oncotarget.27361; PMCID=PMC6925031
Adi Zheng, Nadja Chevalier, Margot Calderoni, Gilles Dubuis, Olivier Dormond, Panos G. Ziros, Gerasimos P. Sykiotis, Christian Widmann;
CRISPR/Cas9 genome-wide screening identifies KEAP1 as a sorafenib, lenvatinib, and regorafenib sensitivity gene in hepatocellular carcinoma.
Oncotarget 10:7058-7070(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023; PMCID=PMC7339254
David Paul Nusinow, John Szpyt, Mahmoud Ghandi, Christopher M. Rose, E. Robert McDonald 3rd, Marian Kalocsay, Judit Jane-Valbuena, Ellen T. Gelfand, Devin K. Schweppe, Mark P. Jedrychowski, Javad Golji ...Show all 20 authors... , Dale A. Porter, Tomas Rejtar, Y. Karen Wang, Gregory V. Kryukov, Frank Stegmeier, Brian K. Erickson, Levi Alexander Garraway, William Raj Sellers, Steven P. Gygi; Show fewer authors
Quantitative proteomics of the Cancer Cell Line Encyclopedia.
Cell 180:387-402.e16(2020)

PubMed=32899426; DOI=10.3390/cancers12092510; PMCID=PMC7565451
Dominique Scherer, Marcela Davila-Lopez, Benjamin Goeppert, Sanna Abrahamsson, Rosa Gonzalez Silos, Igor Nova, Katherine Marcelain, Juan Carlos Roa, David Ibberson, Sinan U. Umu, Trine Ballestad Rounge ...Show all 13 authors... , Stephanie Roessler, Justo Lorenzo-Bermejo; Show fewer authors
RNA sequencing of hepatobiliary cancer cell lines: data and applications to mutational and transcriptomic profiling.
Cancers (Basel) 12:2510.1-2510.14(2020)

PubMed=33193621; DOI=10.3389/fgene.2020.546106; PMCID=PMC7581915
Masaki Kawamoto, Toshiyuki Yamaji, Kyoko Saito, Yoshitaka Shirasago, Kazuhiro Satomura, Toshinori Endo, Masayoshi Fukasawa, Kentaro Hanada, Naoki Osada;
Identification of characteristic genomic markers in human hepatoma Huh-7 and Huh7.5.1-8 cell lines.
Front. Genet. 11:546106.1-546106.10(2020)

PubMed=34320349; DOI=10.1016/j.celrep.2021.109441; PMCID=PMC8333195
Fleur Jochems, Bram Thijssen, Giulia De Conti, Robin Jansen, Ziva Pogacar, Kelvin Groot, Li-Qin Wang, Arnout Schepers, Cun Wang, Hao-Jie Jin, Roderick L. Beijersbergen ...Show all 14 authors... , Rodrigo Leite de Oliveira, Lodewyk F.A. Wessels, Rene Bernards; Show fewer authors
The cancer SENESCopedia: a delineation of cancer cell senescence.
Cell Rep. 36:109441.1-109441.22(2021)

PubMed=34800366; DOI=10.1016/j.cmet.2021.11.001; PMCID=PMC8664129
Marcel Morgenstern, Christian Dominik Peikert, Philipp Lubbert, Ida Suppanz, Cinzia Klemm, Oliver Alka, Conny Steiert, Nataliia Naumenko, Alexander Schendzielorz, Laura Melchionda, Wignand W.D. Muhlhauser ...Show all 29 authors... , Bettina Knapp, Jakob D. Busch, Sebastian B. Stiller, Stefan Dannenmaier, Caroline Lindau, Mariya Licheva, Christopher Eickhorst, Riccardo Galbusera, Ralf M. Zerbes, Michael T. Ryan, Claudine Kraft, Vera Kozjak-Pavlovic, Friedel Drepper, Sven Dennerlein, Silke Oeljeklaus, Nikolaus Pfanner, Nils Wiedemann, Bettina Warscheid; Show fewer authors
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
Cell Metab. 33:2464-2483.e18(2021)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker ...Show all 32 authors... , Jennifer Koh, Howard Lightfoot, Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) ABM; T8973
CCLV; CCLV-RIE 1079
CCTCC; GDC0134
CLS; 300156
JCRB; JCRB0403
KCB; KCB 200970YJ
KCLB; 60104
RCB; RCB1366
RCB; RCB1942
TKG; TKG 0206
Ubigene; YC-D001
Cell line databases/resources CLO; CLO_0009989
CLO; CLO_0050850
CLO; CLO_0050853
MCCL; MCC:0000232
cancercelllines; CVCL_0336
CCRID; 1101HUM-PUMC000679
CCRID; 3101HUMSCSP526
CCRID; 3101HUMTCHu182
CCRID; 5301HUM-KCB09070YJ
Cell_Model_Passport; SIDM00585
CGH-DB; 453-3
CGH-DB; 9072-4
Cosmic-CLP; 907071
DepMap; ACH-000480
FCS-free; 213-2-437-1-3-3
FCS-free; 213-2-540-1-3-3
LIMORE; HuH7
LINCS_LDP; LCL-1926
Lonza; 244
TOKU-E; 3614
Anatomy/cell type resources BTO; BTO_0001950
Biological sample resources BioSample; SAMN03471912
BioSample; SAMN03472130
BioSample; SAMN10987834
ENCODE; ENCBS226AAA
CRISP screens repositories BioGRID_ORCS_Cell_line; 641
Chemistry resources ChEMBL-Cells; CHEMBL3307515
ChEMBL-Targets; CHEMBL614039
GDSC; 907071
PharmacoDB; HuH7_640_2019
PubChem_Cell_line; CVCL_0336
Encyclopedic resources Wikidata; Q27555640
Experimental variables resources EFO; EFO_0005384
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-2706
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
ArrayExpress; E-MTAB-7847
GEO; GSM207048
GEO; GSM388882
GEO; GSM388883
GEO; GSM388884
GEO; GSM481447
GEO; GSM501781
GEO; GSM565880
GEO; GSM816641
GEO; GSM827171
GEO; GSM887149
GEO; GSM888221
GEO; GSM936763
GEO; GSM1178257
GEO; GSM1178258
GEO; GSM1374566
GEO; GSM1669919
GEO; GSM2551570
Polymorphism and mutation databases Cosmic; 871515
Cosmic; 873395
Cosmic; 907071
Cosmic; 945154
Cosmic; 948063
Cosmic; 979730
Cosmic; 1187330
Cosmic; 1351512
Cosmic; 1518226
Cosmic; 1622751
Cosmic; 1995450
Cosmic; 2023863
Cosmic; 2162528
Cosmic; 2321028
Cosmic; 2668283
Cosmic; 2674232
Cosmic; 2771603
Cosmic; 2773182
IARC_TP53; 2011
LiGeA; CCLE_245
Progenetix; CVCL_0336
Proteomic databases PRIDE; PXD001339
PRIDE; PXD005955
PRIDE; PXD008190
PRIDE; PXD023760
PRIDE; PXD028149
PRIDE; PXD028169
PRIDE; PXD028693
PRIDE; PXD030304
Sequence databases EGA; EGAS00001000610
EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number50