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Cellosaurus Huh-7 clone 5-15 (CVCL_C8QS)

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Cell line name Huh-7 clone 5-15
Accession CVCL_C8QS
Resource Identification Initiative To cite this cell line use: Huh-7 clone 5-15 (RRID:CVCL_C8QS)
Comments Population: Japanese.
Virology: Harbors a self-replicating bicistronic hepatitis C virus (HCV) genotype 1b subgenomic replicon (PubMed=10390360; PubMed=11152498).
Doubling time: 25.5 +- 2.5 hours (PubMed=11152498).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Sequence variations
  • Mutation; HGNC; HGNC:6307; KDR; Simple; p.Gln472His (c.1416A>T); ClinVar=VCV000134603; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:20932; POLD3; Simple; p.Lys109Arg (c.326A>G) (p.Lys70Arg, c.209A>G); Zygosity=Unspecified (from parent cell line).
  • Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Tyr220Cys (c.659A>G); ClinVar=VCV000127819; Zygosity=Homozygous (from parent cell line).
Disease Adult hepatocellular carcinoma (NCIt: C7956)
Adult hepatocellular carcinoma (ORDO: Orphanet_210159)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0336 (Huh-7)
Sex of cell Male
Age at sampling 57Y
Category Cancer cell line
Publications

PubMed=10390360; DOI=10.1126/science.285.5424.110
Volker Lohmann, Frank Korner, Jan Oliver Koch, Ulrike Herian, Lorenz Theilmann, Ralf Friedrich Wilhelm Bartenschlager;
Replication of subgenomic hepatitis C virus RNAs in a hepatoma cell line.
Science 285:110-113(1999)

PubMed=11152498; DOI=10.1128/JVI.75.3.1252-1264.2001; PMCID=PMC114031
Thomas Pietschmann, Volker Lohmann, Gabriel Rutter, Katharina Kurpanek, Ralf Friedrich Wilhelm Bartenschlager;
Characterization of cell lines carrying self-replicating hepatitis C virus RNAs.
J. Virol. 75:1252-1264(2001)

Cross-references
Encyclopedic resources Wikidata; Q123032640
Entry history
Entry creation05-Oct-2023
Last entry update14-Aug-2025
Version number5