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Cellosaurus Huh-7.5 (CVCL_7927)

[Text version]
Cell line name Huh-7.5
Synonyms Huh 7.5; Huh7.5
Accession CVCL_7927
Resource Identification Initiative To cite this cell line use: Huh-7.5 (RRID:CVCL_7927)
Comments Part of: ENCODE project common cell types; tier 3.
Population: Japanese.
Virology: Highly permissive for hepatitis C virus (HCV) replication.
Virology: Highly susceptible to infection by Zika virus (ZIKV) (PubMed=29468137).
Omics: Deep proteome analysis.
Omics: Transcriptome analysis by microarray.
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Sequence variations
  • Mutation; HGNC; 6307; KDR; Simple; p.Gln472His (c.1416A>T); ClinVar=VCV000134603; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 20932; POLD3; Simple; p.Lys109Arg (c.326A>G) (p.Lys70Arg, c.209A>G); Zygosity=Unspecified (from parent cell line).
  • Mutation; HGNC; 19102; RIGI; Simple; p.Thr55Ile (c.164C>T); Zygosity=Homozygous (PubMed=15708988).
  • Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Tyr220Cys (c.659A>G); ClinVar=VCV000127819; Zygosity=Homozygous (from parent cell line).
Disease Adult hepatocellular carcinoma (NCIt: C7956)
Adult hepatocellular carcinoma (ORDO: Orphanet_210159)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0336 (Huh-7)
CVCL_C2UW (Huh-7.5 STAT1 KO #1)CVCL_C2UX (Huh-7.5 STAT1 KO #2)CVCL_C2UY (Huh-7.5 STAT2 KO #1)
CVCL_C2UZ (Huh-7.5 STAT2 KO #2)CVCL_A0TI (Huh-7.5 Tet-On)CVCL_E049 (Huh-7.5.1)
Sex of cell Male
Age at sampling 57Y
Category Cancer cell line
Web pages http://genome.ucsc.edu/ENCODE/protocols/cell/human/Huh-7.5_Crawford_protocol.pdf

PubMed=12438626; DOI=10.1128/JVI.76.24.13001-13014.2002
Blight K.J., McKeating J.A., Rice C.M.
Highly permissive cell lines for subgenomic and genomic hepatitis C virus RNA replication.
J. Virol. 76:13001-13014(2002)

PubMed=15708988; DOI=10.1128/JVI.79.5.2689-2699.2005
Sumpter R.M. Jr., Loo Y.-M., Foy E.M., Li K., Yoneyama M., Fujita T., Lemon S.M., Gale M. Jr.
Regulating intracellular antiviral defense and permissiveness to hepatitis C virus RNA replication through a cellular RNA helicase, RIG-I.
J. Virol. 79:2689-2699(2005)

PubMed=17079281; DOI=10.1128/JVI.01534-06
Koutsoudakis G., Herrmann E., Kallis S., Bartenschlager R.F.W., Pietschmann T.
The level of CD81 cell surface expression is a key determinant for productive entry of hepatitis C virus into host cells.
J. Virol. 81:588-598(2007)

PubMed=17552027; DOI=10.3748/wjg.v13.i17.2442
Duverlie G., Wychowski C.
Cell culture systems for the hepatitis C virus.
World J. Gastroenterol. 13:2442-2445(2007)

PubMed=25768906; DOI=10.1016/j.cell.2015.02.025
Luna J.M., Scheel T.K.H., Danino T., Shaw K.S., Mele A., Fak J.J., Nishiuchi E., Takacs C.N., Catanese M.T., de Jong Y.P., Jacobson I.M., Rice C.M., Darnell R.B.
Hepatitis C virus RNA functionally sequesters miR-122.
Cell 160:1099-1110(2015)

PubMed=27929099; DOI=10.1038/srep38336
Yamauchi S., Takeuchi K., Chihara K., Honjoh C., Kato Y., Yoshiki H., Hotta H., Sada K.
STAT1 is essential for the inhibition of hepatitis C virus replication by interferon-lambda but not by interferon-alpha.
Sci. Rep. 6:38336-38336(2016)

PubMed=29468137; DOI=10.5501/wjv.v7.i1.10
Himmelsbach K., Hildt E.
Identification of various cell culture models for the study of Zika virus.
World J. Virol. 7:10-20(2018)

PubMed=30504788; DOI=10.1038/s41598-018-35010-5
Pihl A.F., Offersgaard A.F., Mathiesen C.K., Prentoe J., Fahnoe U., Krarup H.B., Bukh J., Gottwein J.M.
High density Huh7.5 cell hollow fiber bioreactor culture for high-yield production of hepatitis C virus and studies of antivirals.
Sci. Rep. 8:17505-17505(2018)

Cell line databases/resources cancercelllines; CVCL_7927
Lonza; 1432
Anatomy/cell type resources BTO; BTO:0004126
Biological sample resources ENCODE; ENCBS227AAA
CRISP screens repositories BioGRID_ORCS_Cell_line; 1220
Encyclopedic resources Wikidata; Q54896856
Experimental variables resources EFO; EFO_0005704
Gene expression databases GEO; GSM816671
GEO; GSM2247740
GEO; GSM2247741
GEO; GSM2247742
Proteomic databases PRIDE; PXD006386
Entry history
Entry creation04-Apr-2012
Last entry update30-Jan-2024
Version number28