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Cellosaurus Huh-7.5 (CVCL_7927)

[Text version]
Cell line name Huh-7.5
Synonyms Huh 7.5; Huh7.5
Accession CVCL_7927
Resource Identification Initiative To cite this cell line use: Huh-7.5 (RRID:CVCL_7927)
Comments Part of: ENCODE project common cell types; tier 3.
Population: Japanese.
Virology: Highly permissive for hepatitis C virus (HCV) replication.
Virology: Highly susceptible to infection by Zika virus (ZIKV) (PubMed=29468137).
Omics: Proteomics.
Omics: Transcriptomics; Microarray.
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Sequence variations
  • Mutation; HGNC; HGNC:6307; KDR; Simple; p.Gln472His (c.1416A>T); ClinVar=VCV000134603; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:20932; POLD3; Simple; p.Lys109Arg (c.326A>G) (p.Lys70Arg, c.209A>G); Zygosity=Unspecified (from parent cell line).
  • Mutation; HGNC; HGNC:19102; RIGI; Simple; p.Thr55Ile (c.164C>T); Zygosity=Homozygous (PubMed=15708988).
  • Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Tyr220Cys (c.659A>G); ClinVar=VCV000127819; Zygosity=Homozygous (from parent cell line).
Disease Adult hepatocellular carcinoma (NCIt: C7956)
Adult hepatocellular carcinoma (ORDO: Orphanet_210159)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0336 (Huh-7)
Children:
CVCL_C2UW (Huh-7.5 STAT1 KO #1)CVCL_C2UX (Huh-7.5 STAT1 KO #2)CVCL_C2UY (Huh-7.5 STAT2 KO #1)
CVCL_C2UZ (Huh-7.5 STAT2 KO #2)CVCL_A0TI (Huh-7.5 Tet-On)CVCL_E049 (Huh-7.5.1)
Sex of cell Male
Age at sampling 57Y
Category Cancer cell line
Web pages Info; ENCODE; -; http://genome.ucsc.edu/ENCODE/protocols/cell/human/Huh-7.5_Crawford_protocol.pdf
Publications

PubMed=12438626; DOI=10.1128/JVI.76.24.13001-13014.2002; PMCID=PMC136668
Keril J. Blight, Jane Alison McKeating, Charles Moen Rice;
Highly permissive cell lines for subgenomic and genomic hepatitis C virus RNA replication.
J. Virol. 76:13001-13014(2002)

PubMed=15708988; DOI=10.1128/JVI.79.5.2689-2699.2005; PMCID=PMC548482
Rhea Myers Sumpter Jr., Yueh-Ming Loo, Eileen Marie Foy, Kui Li, Mitsutoshi Yoneyama, Takashi Fujita, Stanley M. Lemon, Michael Gale Jr.;
Regulating intracellular antiviral defense and permissiveness to hepatitis C virus RNA replication through a cellular RNA helicase, RIG-I.
J. Virol. 79:2689-2699(2005)

PubMed=17079281; DOI=10.1128/JVI.01534-06; PMCID=PMC1797465
George Koutsoudakis, Eva Herrmann, Stephanie Kallis, Ralf Friedrich Wilhelm Bartenschlager, Thomas Pietschmann;
The level of CD81 cell surface expression is a key determinant for productive entry of hepatitis C virus into host cells.
J. Virol. 81:588-598(2007)

PubMed=17552027; DOI=10.3748/wjg.v13.i17.2442; PMCID=PMC4146762
Gilles Duverlie, Czeslaw Wychowski;
Cell culture systems for the hepatitis C virus.
World J. Gastroenterol. 13:2442-2445(2007)

PubMed=25768906; DOI=10.1016/j.cell.2015.02.025; PMCID=PMC4386883
Joseph M. Luna, Troels K.H. Scheel, Tal Danino, Katharina S. Shaw, Aldo Mele, John J. Fak, Eiko Nishiuchi, Constantin Nicolae Takacs, Maria Teresa Catanese, Ype Peter de Jong, Ira M. Jacobson, Charles Moen Rice, Robert Bernard Darnell; Show fewer authors ...Show all 13 authors...
Hepatitis C virus RNA functionally sequesters miR-122.
Cell 160:1099-1110(2015)

PubMed=27929099; DOI=10.1038/srep38336; PMCID=PMC5144079
Shota Yamauchi, Kenji Takeuchi, Kazuyasu Chihara, Chisato Honjoh, Yuji Kato, Hatsumi Yoshiki, Hak Hotta, Kiyonao Sada;
STAT1 is essential for the inhibition of hepatitis C virus replication by interferon-lambda but not by interferon-alpha.
Sci. Rep. 6:38336-38336(2016)

PubMed=29468137; DOI=10.5501/wjv.v7.i1.10; PMCID=PMC5807893
Kiyoshi Himmelsbach, Eberhard Hildt;
Identification of various cell culture models for the study of Zika virus.
World J. Virol. 7:10-20(2018)

PubMed=30504788; DOI=10.1038/s41598-018-35010-5; PMCID=PMC6269495
Anne Finne Pihl, Anna Falden Offersgaard, Christian K. Mathiesen, Jannick Prentoe, Ulrik Fahnoe, Henrik Bygum Krarup, Jens Bukh, Judith Margarete Gottwein;
High density Huh7.5 cell hollow fiber bioreactor culture for high-yield production of hepatitis C virus and studies of antivirals.
Sci. Rep. 8:17505-17505(2018)

Cross-references
Cell line databases/resources cancercelllines; CVCL_7927
Lonza; 1432
Anatomy/cell type resources BTO; BTO_0004126
Biological sample resources ENCODE; ENCBS227AAA
CRISP screens repositories BioGRID_ORCS_Cell_line; 1220
Encyclopedic resources Wikidata; Q54896856
Experimental variables resources EFO; EFO_0005704
Gene expression databases GEO; GSM816671
GEO; GSM2247740
GEO; GSM2247741
GEO; GSM2247742
Proteomic databases PRIDE; PXD006386
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number30