ID   Huh-7.5
AC   CVCL_7927
SY   Huh 7.5; Huh7.5
DR   BTO; BTO:0004126
DR   EFO; EFO_0005704
DR   BioGRID_ORCS_Cell_line; 1220
DR   cancercelllines; CVCL_7927
DR   ENCODE; ENCBS227AAA
DR   GEO; GSM816671
DR   GEO; GSM2247740
DR   GEO; GSM2247741
DR   GEO; GSM2247742
DR   Lonza; 1432
DR   PRIDE; PXD006386
DR   Wikidata; Q54896856
RX   PubMed=12438626;
RX   PubMed=15708988;
RX   PubMed=17079281;
RX   PubMed=17552027;
RX   PubMed=29468137;
RX   PubMed=25768906;
RX   PubMed=27929099;
RX   PubMed=30504788;
WW   http://genome.ucsc.edu/ENCODE/protocols/cell/human/Huh-7.5_Crawford_protocol.pdf
CC   Part of: ENCODE project common cell types; tier 3.
CC   Population: Japanese.
CC   Virology: Highly permissive for hepatitis C virus (HCV) replication.
CC   Virology: Highly susceptible to infection by Zika virus (ZIKV) (PubMed=29468137).
CC   Sequence variation: Mutation; HGNC; 6307; KDR; Simple; p.Gln472His (c.1416A>T); ClinVar=VCV000134603; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 20932; POLD3; Simple; p.Lys109Arg (c.326A>G) (p.Lys70Arg, c.209A>G); Zygosity=Unspecified (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 19102; RIGI; Simple; p.Thr55Ile (c.164C>T); Zygosity=Homozygous (PubMed=15708988).
CC   Sequence variation: Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11998; TP53; Simple; p.Tyr220Cys (c.659A>G); ClinVar=VCV000127819; Zygosity=Homozygous (from parent cell line).
CC   Omics: Deep proteome analysis.
CC   Omics: Transcriptome analysis by microarray.
CC   Derived from site: In situ; Liver; UBERON=UBERON_0002107.
DI   NCIt; C7956; Adult hepatocellular carcinoma
DI   ORDO; Orphanet_210159; Adult hepatocellular carcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0336 ! Huh-7
SX   Male
AG   57Y
CA   Cancer cell line
DT   Created: 04-04-12; Last updated: 30-01-24; Version: 28
//
RX   PubMed=12438626; DOI=10.1128/JVI.76.24.13001-13014.2002;
RA   Blight K.J., McKeating J.A., Rice C.M.;
RT   "Highly permissive cell lines for subgenomic and genomic hepatitis C
RT   virus RNA replication.";
RL   J. Virol. 76:13001-13014(2002).
//
RX   PubMed=15708988; DOI=10.1128/JVI.79.5.2689-2699.2005;
RA   Sumpter R.M. Jr., Loo Y.-M., Foy E.M., Li K., Yoneyama M., Fujita T.,
RA   Lemon S.M., Gale M. Jr.;
RT   "Regulating intracellular antiviral defense and permissiveness to
RT   hepatitis C virus RNA replication through a cellular RNA helicase,
RT   RIG-I.";
RL   J. Virol. 79:2689-2699(2005).
//
RX   PubMed=17079281; DOI=10.1128/JVI.01534-06;
RA   Koutsoudakis G., Herrmann E., Kallis S., Bartenschlager R.F.W.,
RA   Pietschmann T.;
RT   "The level of CD81 cell surface expression is a key determinant for
RT   productive entry of hepatitis C virus into host cells.";
RL   J. Virol. 81:588-598(2007).
//
RX   PubMed=17552027; DOI=10.3748/wjg.v13.i17.2442;
RA   Duverlie G., Wychowski C.;
RT   "Cell culture systems for the hepatitis C virus.";
RL   World J. Gastroenterol. 13:2442-2445(2007).
//
RX   PubMed=29468137; DOI=10.5501/wjv.v7.i1.10;
RA   Himmelsbach K., Hildt E.;
RT   "Identification of various cell culture models for the study of Zika
RT   virus.";
RL   World J. Virol. 7:10-20(2018).
//
RX   PubMed=25768906; DOI=10.1016/j.cell.2015.02.025;
RA   Luna J.M., Scheel T.K.H., Danino T., Shaw K.S., Mele A., Fak J.J.,
RA   Nishiuchi E., Takacs C.N., Catanese M.T., de Jong Y.P., Jacobson I.M.,
RA   Rice C.M., Darnell R.B.;
RT   "Hepatitis C virus RNA functionally sequesters miR-122.";
RL   Cell 160:1099-1110(2015).
//
RX   PubMed=27929099; DOI=10.1038/srep38336;
RA   Yamauchi S., Takeuchi K., Chihara K., Honjoh C., Kato Y., Yoshiki H.,
RA   Hotta H., Sada K.;
RT   "STAT1 is essential for the inhibition of hepatitis C virus
RT   replication by interferon-lambda but not by interferon-alpha.";
RL   Sci. Rep. 6:38336-38336(2016).
//
RX   PubMed=30504788; DOI=10.1038/s41598-018-35010-5;
RA   Pihl A.F., Offersgaard A.F., Mathiesen C.K., Prentoe J., Fahnoe U.,
RA   Krarup H.B., Bukh J., Gottwein J.M.;
RT   "High density Huh7.5 cell hollow fiber bioreactor culture for
RT   high-yield production of hepatitis C virus and studies of
RT   antivirals.";
RL   Sci. Rep. 8:17505-17505(2018).
//