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Cellosaurus Huh-7.5 Tet-On (CVCL_A0TI)

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Cell line name Huh-7.5 Tet-On
Synonyms Huh-7.5 TetON
Accession CVCL_A0TI
Resource Identification Initiative To cite this cell line use: Huh-7.5 Tet-On (RRID:CVCL_A0TI)
Comments Population: Japanese.
Characteristics: Transfected with reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 (HHV-1) VP16 (UL28) (Note=With p.Met1_Arg360del).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Sequence variations
  • Mutation; HGNC; HGNC:6307; KDR; Simple; p.Gln472His (c.1416A>T); ClinVar=VCV000134603; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:20932; POLD3; Simple; p.Lys109Arg (c.326A>G) (p.Lys70Arg, c.209A>G); Zygosity=Unspecified (from parent cell line).
  • Mutation; HGNC; HGNC:19102; RIGI; Simple; p.Thr55Ile (c.164C>T); Zygosity=Unspecified (from parent cell line).
  • Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Tyr220Cys (c.659A>G); ClinVar=VCV000127819; Zygosity=Homozygous (from parent cell line).
Disease Adult hepatocellular carcinoma (NCIt: C7956)
Adult hepatocellular carcinoma (ORDO: Orphanet_210159)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_7927 (Huh-7.5)
Sex of cell Male
Age at sampling 57Y
Category Cancer cell line
STR profile Source(s): Millipore=SCC265

Markers:
AmelogeninX
CSF1PO11
D3S135815
D5S81812
D7S82010,11
D8S117914
D13S31710,11
D16S53910
D18S5115
D21S1130
FGA22
Penta D12
Penta E11
TH017
TPOX8,11
vWA16,18

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Publications

PubMed=25768906; DOI=10.1016/j.cell.2015.02.025; PMCID=PMC4386883
Joseph M. Luna, Troels Kasper Hoyer Scheel, Tal Danino, Katharina S. Shaw, Aldo Mele, John J. Fak, Eiko Nishiuchi, Constantin Nicolae Takacs, Maria Teresa Catanese, Ype Peter de Jong, Ira M. Jacobson ...Show all 13 authors... , Charles Moen Rice, Robert Bernard Darnell; Show fewer authors
Hepatitis C virus RNA functionally sequesters miR-122.
Cell 160:1099-1110(2015)

Cross-references
Cell line collections (Providers) Millipore; SCC265
Cell line databases/resources cancercelllines; CVCL_A0TI
Encyclopedic resources Wikidata; Q108820508
Entry history
Entry creation23-Sep-2021
Last entry update14-Aug-2025
Version number11