ID   Huh-7.5 Tet-On
AC   CVCL_A0TI
SY   Huh-7.5 TetON
DR   cancercelllines; CVCL_A0TI
DR   Millipore; SCC265
DR   Wikidata; Q108820508
RX   PubMed=25768906;
CC   Population: Japanese.
CC   Characteristics: Transfected with reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16.
CC   Sequence variation: Mutation; HGNC; 6307; KDR; Simple; p.Gln472His (c.1416A>T); ClinVar=VCV000134603; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 20932; POLD3; Simple; p.Lys109Arg (c.326A>G) (p.Lys70Arg, c.209A>G); Zygosity=Unspecified (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 19102; RIGI; Simple; p.Thr55Ile (c.164C>T); Zygosity=Unspecified (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11998; TP53; Simple; p.Tyr220Cys (c.659A>G); ClinVar=VCV000127819; Zygosity=Homozygous (from parent cell line).
CC   Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del).
CC   Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from site: In situ; Liver; UBERON=UBERON_0002107.
ST   Source(s): Millipore=SCC265
ST   Amelogenin: X
ST   CSF1PO: 11
ST   D13S317: 10,11
ST   D16S539: 10
ST   D18S51: 15
ST   D21S11: 30
ST   D3S1358: 15
ST   D5S818: 12
ST   D7S820: 10,11
ST   D8S1179: 14
ST   FGA: 22
ST   Penta D: 12
ST   Penta E: 11
ST   TH01: 7
ST   TPOX: 8,11
ST   vWA: 16,18
DI   NCIt; C7956; Adult hepatocellular carcinoma
DI   ORDO; Orphanet_210159; Adult hepatocellular carcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_7927 ! Huh-7.5
SX   Male
AG   57Y
CA   Cancer cell line
DT   Created: 23-09-21; Last updated: 02-05-24; Version: 8
//
RX   PubMed=25768906; DOI=10.1016/j.cell.2015.02.025;
RA   Luna J.M., Scheel T.K.H., Danino T., Shaw K.S., Mele A., Fak J.J.,
RA   Nishiuchi E., Takacs C.N., Catanese M.T., de Jong Y.P., Jacobson I.M.,
RA   Rice C.M., Darnell R.B.;
RT   "Hepatitis C virus RNA functionally sequesters miR-122.";
RL   Cell 160:1099-1110(2015).
//