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Cellosaurus RAW 264.7 (CVCL_0493)

[Text version]
Cell line name RAW 264.7
Synonyms RAW264; RAW2647; RAW264.7; RAW-264.7; Raw 264.7; Raw264.7
Accession CVCL_0493
Resource Identification Initiative To cite this cell line use: RAW 264.7 (RRID:CVCL_0493)
Comments Part of: Tumor Immunology Bank (TIB) collection from Salk (transferred to ATCC in 1981).
Virology: Not susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257).
Doubling time: 11 hours (Note=From cell counting), 12 hours (Note=From absorbance) (DOI=10.5897/IJBMBR2013.0154); ~30 hours (CLS=400319).
Transformant: NCBI_TaxID; 11788; Abelson mouse leukemia virus (AbMuLV).
Omics: Metabolomics.
Omics: Proteomics; Subcellular; Phagosome.
Omics: Proteomics; Subcellular; Phagosome; Quantitative.
Omics: Variations; SNP array analysis.
Miscellaneous: PubMed=23430347 has a different value for STR 6-4 (17) than that of NIST (18) due to a change in the marker motif (personal communication of Almeida, Jamie L.).
Misspelling: RAW 267.4; Note=Occasionally.
Misspelling: Raw 267.4; Note=Occasionally.
Misspelling: RAW267.4; Note=Occasionally.
Misspelling: RAW 274; Note=Occasionally.
Misspelling: RAW-274; Note=Occasionally.
Misspelling: RAW274; Note=Occasionally.
Derived from site: In situ; Ascites; UBERON=UBERON_0007795.
Cell type: Macrophage; CL=CL_0000235.
Disease Mouse leukemia (NCIt: C21604)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: BALB/c.
Hierarchy Parent: CVCL_4478 (RAW 264)
Children:
CVCL_B0YM (Abcam RAW 264.7 Ccl7 KO)CVCL_B7VG (Abcam RAW 264.7 Ccr2 KO)CVCL_B0YN (Abcam RAW 264.7 Cd68 KO)
CVCL_B7VK (Abcam RAW 264.7 Cdkn1b KO)CVCL_B7VH (Abcam RAW 264.7 Cx3cr1 KO)CVCL_B0YP (Abcam RAW 264.7 Prkaa1 KO)
CVCL_B7VI (Abcam RAW 264.7 Sirpa KO)CVCL_D7C8 (Abeomics RAW264.7 GFP)CVCL_KB45 (CellSensor NFkB-bla RAW 264.7)
CVCL_DD02 (EA13.5)CVCL_7189 (eCAS)CVCL_E1MJ (HyCyte RAW 264.7 KO-mBcl2)
CVCL_D6CE (HyCyte RAW 264.7 KO-mCd274)CVCL_E1MK (HyCyte RAW 264.7 KO-mClec4e)CVCL_D6CF (HyCyte RAW 264.7 KO-mErlin1)
CVCL_E1ML (HyCyte RAW 264.7 KO-mLy96)CVCL_E1MM (HyCyte RAW 264.7 KO-mMcfd2)CVCL_D6CG (HyCyte RAW 264.7 KO-mMettl1)
CVCL_E1MN (HyCyte RAW 264.7 KO-mMir146)CVCL_D6CH (HyCyte RAW 264.7 KO-mPrdm2)CVCL_D6CI (HyCyte RAW 264.7 KO-mSamhd1)
CVCL_E1MP (HyCyte RAW 264.7 KO-mSetdb2)CVCL_E1MQ (HyCyte RAW 264.7 KO-mSsty2)CVCL_E3II (HyCyte RAW 264.7-Luc)
CVCL_HF55 (ImKC)CVCL_D7AI (Leeporter RAW264.7 IL-8 luciferase)CVCL_D7AJ (Leeporter RAW264.7 INOS luciferase)
CVCL_D7AK (Leeporter RAW264.7 ISRE luciferase)CVCL_D7AL (Leeporter RAW264.7 MIP-2 luciferase)CVCL_D7AM (Leeporter RAW264.7 NF-kB luciferase)
CVCL_D7AN (Leeporter RAW264.7 NFAT luciferase)CVCL_D7AP (Leeporter RAW264.7 STAT1 luciferase)CVCL_D7AQ (Leeporter RAW264.7 STAT3 luciferase)
CVCL_D7AR (Leeporter RAW264.7 TNF-alpha luciferase)CVCL_D7AS (Leeporter RAW264.7 TNF-beta luciferase)CVCL_B417 (M9A)
CVCL_C8RU (RAW 264.7 Ern1 KO)CVCL_6517 (RAW 264.7 gammaNO(-))CVCL_C8RT (RAW 264.7 Gba KO)
CVCL_F0Q7 (RAW 264.7 Gys1 KO)CVCL_F0Q8 (RAW 264.7 Gys1-Flag OE)CVCL_C8HB (RAW 264.7 Irf3 KO)
CVCL_UL72 (RAW 264.7 LRRK2 KO)CVCL_C8HC (RAW 264.7 Lrrk2 KO [Montreal])CVCL_UL71 (RAW 264.7 LRRK2 parental)
CVCL_UL73 (RAW 264.7 LRRK2 T1348N mut)CVCL_C8RV (RAW 264.7 Ppm1h KO)CVCL_C8RW (RAW 264.7 Rab10 KO)
CVCL_C8RX (RAW 264.7 Rab29 KO)CVCL_C8RY (RAW 264.7 Rab8a KO)CVCL_ZD89 (RAW 264.7 shRNA-RSK2)
CVCL_D7F3 (RAW 264.7 Sting1 KO)CVCL_C8HE (RAW 264.7 Sting1 R330A/R333A)CVCL_C8WQ (RAW 264.7-EGFP)
CVCL_6717 (RAW 264.7/LR5)CVCL_A7ZG (RAW-ASC)CVCL_X594 (RAW-Blue)
CVCL_X595 (RAW-Blue ISG)CVCL_F681 (RAW-D)CVCL_A8CB (RAW-Difluo mLC3)
CVCL_A7ZK (RAW-Dual)CVCL_C3NB (RAW-E6)CVCL_C3NC (RAW-EGFP)
CVCL_C8H9 (RAW-Kb)CVCL_X596 (RAW-Lucia ISG)CVCL_C6WW (RAW-N)
CVCL_9W02 (RAW-TT10)CVCL_1P00 (RAW264-TG3)CVCL_C6WZ (RAW264.7 clone C1)
CVCL_C6X8 (RAW264.7 clone C10)CVCL_C6X9 (RAW264.7 clone C11)CVCL_C6XA (RAW264.7 clone C12)
CVCL_C6X0 (RAW264.7 clone C2)CVCL_C6X1 (RAW264.7 clone C3)CVCL_C6X2 (RAW264.7 clone C4)
CVCL_C6X3 (RAW264.7 clone C5)CVCL_C6X4 (RAW264.7 clone C6)CVCL_C6X5 (RAW264.7 clone C7)
CVCL_C6X6 (RAW264.7 clone C8)CVCL_C6X7 (RAW264.7 clone C9)CVCL_C6WX (RAW264.7 clone H9)
CVCL_C6WY (RAW264.7 clone J8)CVCL_C6XG (RAW264.7/HNP)CVCL_E1AX (Ubigene RAW 264.7 AA467197 KO)
CVCL_E1AY (Ubigene RAW 264.7 Ahr KO)CVCL_E1AZ (Ubigene RAW 264.7 C3ar1 KO)CVCL_E1B0 (Ubigene RAW 264.7 Ccdc115 KO)
CVCL_E1B1 (Ubigene RAW 264.7 Cd180 KO)CVCL_E1B2 (Ubigene RAW 264.7 Cdkn1b KO)CVCL_E1B3 (Ubigene RAW 264.7 Cgas KO)
CVCL_E1B4 (Ubigene RAW 264.7 Chrna7 KO)CVCL_E1B5 (Ubigene RAW 264.7 Coro1a KO)CVCL_E1B6 (Ubigene RAW 264.7 Csf3 KO)
CVCL_E1B7 (Ubigene RAW 264.7 Dancr KO)CVCL_E1B8 (Ubigene RAW 264.7 Fabp5 KO)CVCL_E1B9 (Ubigene RAW 264.7 Fcer1g KO)
CVCL_E1BA (Ubigene RAW 264.7 Fgfr3 KO)CVCL_E1BB (Ubigene RAW 264.7 Gpx4 KO)CVCL_E1BC (Ubigene RAW 264.7 Hif1a KO)
CVCL_E1BD (Ubigene RAW 264.7 Hmgb1 KO)CVCL_E1BE (Ubigene RAW 264.7 Hsd11b1 KO)CVCL_E1BF (Ubigene RAW 264.7 Ift140 KO)
CVCL_E1BG (Ubigene RAW 264.7 Lgals8 KO)CVCL_E1BH (Ubigene RAW 264.7 Lgr4 KO)CVCL_E1BI (Ubigene RAW 264.7 Mirlet7f-1 KO)
CVCL_E1BJ (Ubigene RAW 264.7 Myd88 KO)CVCL_E1BK (Ubigene RAW 264.7 Myl12a KO)CVCL_E1BL (Ubigene RAW 264.7 Ncf1 KO)
CVCL_E1BM (Ubigene RAW 264.7 Nfe2l2 KO)CVCL_E1BN (Ubigene RAW 264.7 Pik3r1 KO)CVCL_E1BP (Ubigene RAW 264.7 Ppt1 KO)
CVCL_E1BQ (Ubigene RAW 264.7 Ptpn11 KO)CVCL_E1BR (Ubigene RAW 264.7 Rheb KO)CVCL_E1BS (Ubigene RAW 264.7 S100a8 KO)
CVCL_E1BT (Ubigene RAW 264.7 Sat1 KO)CVCL_E1BU (Ubigene RAW 264.7 Sdha KO)CVCL_E1BV (Ubigene RAW 264.7 Siglec15 KO)
CVCL_E1BW (Ubigene RAW 264.7 Sqstm1 KO)CVCL_E1BX (Ubigene RAW 264.7 Tbk1 KO)CVCL_E1BY (Ubigene RAW 264.7 Tsc2 KO)
CVCL_E1BZ (Ubigene RAW 264.7 Zdhhc13 KO)
Sex of cell Male
Age at sampling Adult
Category Cancer cell line
STR profile Source(s): PubMed=23430347; PubMed=31220119; Ubigene=YC-C020

Markers:
Mouse STR 1-115,16
Mouse STR 1-217
Mouse STR 2-116
Mouse STR 3-214
Mouse STR 4-222.3
Mouse STR 5-514
Mouse STR 6-418
Mouse STR 6-712
Mouse STR 7-125.2
Mouse STR 8-113
Mouse STR 9-215
Mouse STR 11-217
Mouse STR 12-116
Mouse STR 13-116.2
Mouse STR 15-322.3
Mouse STR 17-214,16
Mouse STR 18-318
Mouse STR 19-214
Mouse STR X-124

Run an STR similarity search on this cell line
Web pages Info; -; -; https://web.archive.org/web/20201109215324/www.infarktforschung.de/macrophages_cell_lines.html
Info; ATCC; -; https://www.atcc.org/en/support/technical-support/faqs/morphology-of-atcc-tib-71
Info; ATCC; -; https://www.atcc.org/en/support/technical-support/faqs/tib-71-growth-and-morphology
Info; COPE; -; http://www.cells-talk.com/index.php/page/copelibrary?key=RAW264.7
Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/r/cell-lines-detail-325.html
Publications

PubMed=212198; DOI=10.1016/0092-8674(78)90101-0
William C. Raschke, Stephen M. Baird, Peter Ralph, Ilona Nakoinz;
Functional macrophage cell lines transformed by Abelson leukemia virus.
Cell 15:261-267(1978)

PubMed=3792703; DOI=10.1111/j.1432-0436.1986.tb00568.x
Pieter J.M. Leenen, Anita M.A.C. Jansen, Willem van Ewijk;
Murine macrophage cell lines can be ordered in a linear differentiation sequence.
Differentiation 32:157-164(1986)

PubMed=16948499; DOI=10.1290/0510075.1; PMCID=PMC2883882
Bethany L.V. Cuetara, Tania N. Crotti, Anthony J. O'Donoghue, Kevin P. McHugh;
Cloning and characterization of osteoclast precursors from the RAW264.7 cell line.
In Vitro Cell. Dev. Biol. Anim. 42:182-188(2006)

PubMed=19057837; DOI=10.1007/s00774-008-0018-6
Cristina Vincent, Masakazu Kogawa, David M. Findlay, Gerald J. Atkins;
The generation of osteoclasts from RAW 264.7 precursors in defined, serum-free conditions.
J. Bone Miner. Metab. 27:114-119(2009)

PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006
Matthias Trost, Luc English, Sebastien Lemieux, Mathieu Courcelles, Michel Desjardins, Pierre Thibault;
The phagosomal proteome in interferon-gamma-activated macrophages.
Immunity 30:143-154(2009)

PubMed=22735334; DOI=10.1038/msb.2012.21; PMCID=PMC3397418
Aarash Bordbar, Monica L. Mo, Ernesto Satoshi Nakayasu, Alexandra C. Schrimpe-Rutledge, Young-Mo Kim, Thomas O. Metz, Marcus B. Jones, Bryan C. Frank, Richard D. Smith, Scott N. Peterson, Daniel R. Hyduke ...Show all 13 authors... , Joshua N. Adkins, Bernhard Orn Palsson; Show fewer authors
Model-driven multi-omic data analysis elucidates metabolic immunomodulators of macrophage activation.
Mol. Syst. Biol. 8:558-558(2012)

DOI=10.5897/IJBMBR2013.0154
Simon Bernard Iloki Assanga, Armida Andrea Gil-Salido, Lydianis Maria Lewis Lujan, Aaron de Jesus Rosas-Durazo, Ana Lilian Acosta-Silva, Elba Griselda Rivera-Castaneda, Jose Luis Rubio-Pino;
Cell growth curves for different cell lines and their relationship with biological activities.
Int. J. Biotechnol. Mol. Biol. Res. 4:60-70(2013)

PubMed=23430347; DOI=10.1007/s10616-013-9545-7; PMCID=PMC3886540
Jamie L. Almeida, Carolyn R. Hill, Kenneth D. Cole;
Mouse cell line authentication.
Cytotechnology 66:133-147(2014)

PubMed=25277546; DOI=10.1186/1471-2164-15-847; PMCID=PMC4198738
John Paul Didion, Ryan J. Buus, Zohreh Naghashfar, David W. Threadgill, Herbert Carpenter Morse 3rd, Fernando Pardo-Manuel de Villena;
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847.1-847.11(2014)

PubMed=25504905; DOI=10.1002/pmic.201400431; PMCID=PMC4833182
Man-Man Guo, Anetta Svitorka Hartlova, Brian D. Dill, Alan R. Prescott, Marek Gierlinski, Matthias Trost;
High-resolution quantitative proteome analysis reveals substantial differences between phagosomes of RAW 264.7 and bone marrow derived macrophages.
Proteomics 15:3169-3174(2015)

PubMed=29889899; DOI=10.1371/journal.pone.0198943; PMCID=PMC5995401
Bartoomiej Taciak, Maciej Bialasek, Agata Braniewska, Zuzanna Sas, Paulina Sawicka, Lukasz Kiraga, Tomasz Piotr Rygiel, Magdalena Krol;
Evaluation of phenotypic and functional stability of RAW 264.7 cell line through serial passages.
PLoS ONE 13:e0198943.1-e0198943.13(2018)

PubMed=31220119; DOI=10.1371/journal.pone.0218412; PMCID=PMC6586308
Jamie L. Almeida, Aleksandra Dakic, Karin Kindig, Maikan Kone, Deborah Lee Dormady Letham, Scott Langdon, Ruth Peat, Jayamalini Holding-Pillai, Erin M. Hall, Mark Ladd, Megan D. Shaffer ...Show all 23 authors... , Heath Berg, Jin-Liang Li, Georges Wigger, Steve Lund, Carolyn R. Steffen, Barbara B. Fransway, Bob Geraghty, Manuela Natoli, Beth A. Bauer, Susanne M. Gollin, Dale W. Lewis, Yvonne A. Reid; Show fewer authors
Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.
PLoS ONE 14:e0218412.1-e0218412.24(2019)

PubMed=31947698; DOI=10.3390/ijms21020538; PMCID=PMC7013577
Laia Mira-Pascual, Anh N. Tran, Goran Andersson, Tuomas Nareoja, Pernilla Lang;
A sub-clone of RAW264.7-cells form osteoclast-like cells capable of bone resorption faster than parental RAW264.7 through increased de novo expression and nuclear translocation of NFATc1.
Int. J. Mol. Sci. 21:538.1-538.19(2020)

PubMed=33389257; DOI=10.1007/s10096-020-04106-0; PMCID=PMC7778494
Nathalie Wurtz, Gwilherm Penant, Priscilla Jardot, Nathalie Duclos, Bernard La Scola;
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)

Cross-references
Cell line collections (Providers) Abcam; ab275474
AddexBio; C0003008/444
ATCC; TIB-71
BCRC; 60001
BCRJ; 0212
CCTCC; GDC0143
CLS; 400319
ECACC; 91062702
IBRC; C10072
ICLC; ATL02001
IZSLER; BS TCL 177
KCB; KCB 200603YJ
KCLB; 40071
NCBI_Iran; C639
Ubigene; YC-C020
Cell line databases/resources CLO; CLO_0008753
CLO; CLO_0051424
MCCL; MCC:0000390
CLDB; cl4110
CLDB; cl7129
CCRID; 1101MOU-PUMC000146
CCRID; 3101MOUSCSP5036
CCRID; 3101MOUTCM13
CCRID; 4201MOU-CCTCC00143
CCRID; 6101MOU-CERC000109
FCS-free; 225-5-468-2-3-3
Lonza; 674
TOKU-E; 2976
TOKU-E; 4095
Anatomy/cell type resources BTO; BTO_0003292
Biological sample resources BioSample; SAMN11397665
Chemistry resources ChEMBL-Cells; CHEMBL3307977
ChEMBL-Targets; CHEMBL612557
PubChem_Cell_line; CVCL_0493
Encyclopedic resources Wikidata; Q28335461
Experimental variables resources EFO; EFO_0001231
Medical resources MeSH; D000067996
Metabolomic databases MetaboLights; MTBLS23
MetaboLights; MTBL1140
MetaboLights; MTBLS636
MetaboLights; MTBLS696
Proteomic databases PRIDE; PXD000984
PRIDE; PXD001293
PRIDE; PXD001905
PRIDE; PXD001935
PRIDE; PXD003545
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number41