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Cellosaurus RAW 264.7 (CVCL_0493)

[Text version]
Cell line name RAW 264.7
Synonyms RAW264; RAW2647; RAW264.7; RAW-264.7; Raw 264.7; Raw264.7
Accession CVCL_0493
Resource Identification Initiative To cite this cell line use: RAW 264.7 (RRID:CVCL_0493)
Comments Part of: Tumor Immunology Bank (TIB) collection from Salk (transferred to ATCC in 1981).
Virology: Not susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257).
Doubling time: 11 hours (from cell counting), 12 hours (from absorbance) (DOI=10.5897/IJBMBR2013.0154); ~30 hours (CLS).
Transformant: NCBI_TaxID; 11788; Abelson mouse leukemia virus (AbMuLV).
Omics: Metabolome analysis.
Omics: Phagosome quantitative phosphoproteome analysis.
Omics: Phagosome analysis by proteomics.
Omics: SNP array analysis.
Miscellaneous: PubMed=23430347 has a different value for STR 6-4 (17) than that of NIST (18) due to a change in the marker motif (personal communication of Almeida J.L.).
Misspelling: RAW 267.4; Note=Occasionally.
Misspelling: Raw 267.4; Note=Occasionally.
Misspelling: RAW267.4; Note=Occasionally.
Misspelling: RAW 274; Note=Occasionally.
Misspelling: RAW-274; Note=Occasionally.
Misspelling: RAW274; Note=Occasionally.
Derived from sampling site: Ascites. Cell type=Macrophage.
Disease Mouse leukemia (NCIt: C21604)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: BALB/c.
Hierarchy Parent: CVCL_4478 (RAW 264)
Children:
CVCL_B0YM (Abcam RAW 264.7 Ccl7 KO)CVCL_B7VG (Abcam RAW 264.7 Ccr2 KO)CVCL_B0YN (Abcam RAW 264.7 Cd68 KO)
CVCL_B7VK (Abcam RAW 264.7 Cdkn1b KO)CVCL_B7VH (Abcam RAW 264.7 Cx3cr1 KO)CVCL_B0YP (Abcam RAW 264.7 Prkaa1 KO)
CVCL_B7VI (Abcam RAW 264.7 Sirpa KO)CVCL_KB45 (CellSensor NFkB-bla RAW 264.7)CVCL_DD02 (EA13.5)
CVCL_7189 (eCAS)CVCL_HF55 (ImKC)CVCL_B417 (M9A)
CVCL_6517 (RAW 264.7 gammaNO(-))CVCL_UL72 (RAW 264.7 LRRK2 KO)CVCL_UL71 (RAW 264.7 LRRK2 parental)
CVCL_UL73 (RAW 264.7 LRRK2 T1348N mut)CVCL_ZD89 (RAW 264.7 shRNA-RSK2)CVCL_6717 (RAW 264.7/LR5)
CVCL_A7ZG (RAW-ASC)CVCL_X594 (RAW-Blue)CVCL_X595 (RAW-Blue ISG)
CVCL_F681 (RAW-D)CVCL_A8CB (RAW-Difluo mLC3)CVCL_A7ZK (RAW-Dual)
CVCL_C3NB (RAW-E6)CVCL_C3NC (RAW-EGFP)CVCL_X596 (RAW-Lucia ISG)
CVCL_C6WW (RAW-N)CVCL_9W02 (RAW-TT10)CVCL_1P00 (RAW264-TG3)
CVCL_C6WZ (RAW264.7 clone C1)CVCL_C6X8 (RAW264.7 clone C10)CVCL_C6X9 (RAW264.7 clone C11)
CVCL_C6XA (RAW264.7 clone C12)CVCL_C6X0 (RAW264.7 clone C2)CVCL_C6X1 (RAW264.7 clone C3)
CVCL_C6X2 (RAW264.7 clone C4)CVCL_C6X3 (RAW264.7 clone C5)CVCL_C6X4 (RAW264.7 clone C6)
CVCL_C6X5 (RAW264.7 clone C7)CVCL_C6X6 (RAW264.7 clone C8)CVCL_C6X7 (RAW264.7 clone C9)
CVCL_C6WX (RAW264.7 clone H9)CVCL_C6WY (RAW264.7 clone J8)CVCL_C6XG (RAW264.7/HNP)
Sex of cell Male
Age at sampling Adult
Category Cancer cell line
STR profile Source(s): PubMed=23430347; PubMed=31220119

Markers:
Mouse STR 1-115,16
Mouse STR 1-217
Mouse STR 2-116
Mouse STR 3-214
Mouse STR 4-222.3
Mouse STR 5-514
Mouse STR 6-418
Mouse STR 6-712
Mouse STR 7-125.2
Mouse STR 8-113
Mouse STR 9-215
Mouse STR 11-217
Mouse STR 12-116
Mouse STR 13-116.2
Mouse STR 15-322.3
Mouse STR 17-214,16
Mouse STR 18-318
Mouse STR 19-214
Mouse STR X-124

Run an STR similarity search on this cell line
Web pages http://www.cells-talk.com/index.php/page/copelibrary?key=RAW264.7
https://www.atcc.org/en/support/technical-support/faqs/morphology-of-atcc-tib-71
https://www.atcc.org/en/support/technical-support/faqs/tib-71-growth-and-morphology
https://web.archive.org/web/20201109215324/www.infarktforschung.de/macrophages_cell_lines.html
https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/r/cell-lines-detail-325.html
Publications

PubMed=212198; DOI=10.1016/0092-8674(78)90101-0
Raschke W.C., Baird S., Ralph P., Nakoinz I.
Functional macrophage cell lines transformed by Abelson leukemia virus.
Cell 15:261-267(1978)

PubMed=3792703; DOI=10.1111/j.1432-0436.1986.tb00568.x
Leenen P.J.M., Jansen A.M.A.C., van Ewijk W.
Murine macrophage cell lines can be ordered in a linear differentiation sequence.
Differentiation 32:157-164(1986)

PubMed=16948499; DOI=10.1290/0510075.1
Cuetara B.L.V., Crotti T.N., O'Donoghue A.J., McHugh K.P.
Cloning and characterization of osteoclast precursors from the RAW264.7 cell line.
In Vitro Cell. Dev. Biol. Anim. 42:182-188(2006)

PubMed=19057837; DOI=10.1007/s00774-008-0018-6
Vincent C., Kogawa M., Findlay D.M., Atkins G.J.
The generation of osteoclasts from RAW 264.7 precursors in defined, serum-free conditions.
J. Bone Miner. Metab. 27:114-119(2009)

PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
The phagosomal proteome in interferon-gamma-activated macrophages.
Immunity 30:143-154(2009)

PubMed=22735334; DOI=10.1038/msb.2012.21
Bordbar A., Mo M.L., Nakayasu E.S., Schrimpe-Rutledge A.C., Kim Y.-M., Metz T.O., Jones M.B., Frank B.C., Smith R.D., Peterson S.N., Hyduke D.R., Adkins J.N., Palsson B.O.
Model-driven multi-omic data analysis elucidates metabolic immunomodulators of macrophage activation.
Mol. Syst. Biol. 8:558-558(2012)

DOI=10.5897/IJBMBR2013.0154
Iloki Assanga S.B., Gil-Salido A.A., Lewis Lujan L.M., Rosas-Durazo A., Acosta-Silva A.L., Rivera-Castaneda E.G., Rubio-Pino J.L.
Cell growth curves for different cell lines and their relationship with biological activities.
Int. J. Biotechnol. Mol. Biol. Res. 4:60-70(2013)

PubMed=23430347; DOI=10.1007/s10616-013-9545-7
Almeida J.L., Hill C.R., Cole K.D.
Mouse cell line authentication.
Cytotechnology 66:133-147(2014)

PubMed=25277546; DOI=10.1186/1471-2164-15-847
Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. III, Pardo-Manuel de Villena F.
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847.1-847.11(2014)

PubMed=25504905; DOI=10.1002/pmic.201400431
Guo M., Hartlova A., Dill B.D., Prescott A.R., Gierlinski M., Trost M.
High-resolution quantitative proteome analysis reveals substantial differences between phagosomes of RAW 264.7 and bone marrow derived macrophages.
Proteomics 15:3169-3174(2015)

PubMed=29889899; DOI=10.1371/journal.pone.0198943
Taciak B., Bialasek M., Braniewska A., Sas Z., Sawicka P., Kiraga L., Rygiel T., Krol M.
Evaluation of phenotypic and functional stability of RAW 264.7 cell line through serial passages.
PLoS ONE 13:E0198943-E0198943(2018)

PubMed=31220119; DOI=10.1371/journal.pone.0218412
Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S., Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H., Li J.-L., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B., Natoli M., Bauer B., Gollin S.M., Lewis D.W., Reid Y.A.
Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.
PLoS ONE 14:E0218412-E0218412(2019)

PubMed=31947698; DOI=10.3390/ijms21020538
Mira-Pascual L., Tran A.N., Andersson G., Nareoja T., Lang P.
A sub-clone of RAW264.7-cells form osteoclast-like cells capable of bone resorption faster than parental RAW264.7 through increased de novo expression and nuclear translocation of NFATc1.
Int. J. Mol. Sci. 21:538.1-538.19(2020)

PubMed=33389257; DOI=10.1007/s10096-020-04106-0
Wurtz N., Penant G., Jardot P., Duclos N., La Scola B.
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)

Cross-references
Cell line collections Abcam; ab275474
AddexBio; C0003008/444
ATCC; TIB-71
BCRC; 60001
BCRJ; 0212
CCTCC; GDC0143
CLS; 400319
ECACC; 91062702
IBRC; C10072
ICLC; ATL02001
IZSLER; BS TCL 177
KCB; KCB 200603YJ
KCLB; 40071
NCBI_Iran; C639
Cell line databases/resources CLO; CLO_0008753
CLO; CLO_0051424
MCCL; MCC:0000390
CLDB; cl4110
CLDB; cl7129
CCRID; 1101MOU-PUMC000146
CCRID; 3101MOUSCSP5036
CCRID; 3101MOUTCM13
CCRID; 4201MOU-CCTCC00143
CCRID; 6101MOU-CERC000109
FCS-free; 225-5-468-2-3-3
Lonza; 674
TOKU-E; 2976
TOKU-E; 4095
Anatomy/cell type resources BTO; BTO:0003292
Biological sample resources BioSample; SAMN11397665
Chemistry resources ChEMBL-Cells; CHEMBL3307977
ChEMBL-Targets; CHEMBL612557
PubChem_Cell_line; CVCL_0493
Encyclopedic resources Wikidata; Q28335461
Experimental variables resources EFO; EFO_0001231
Medical resources MeSH; D000067996
Metabolomic databases MetaboLights; MTBLS23
MetaboLights; MTBLS636
MetaboLights; MTBLS696
MetaboLights; MTBL1140
Proteomic databases PRIDE; PXD000984
PRIDE; PXD001293
PRIDE; PXD001905
PRIDE; PXD001935
PRIDE; PXD003545
Entry history
Entry creation04-Apr-2012
Last entry update21-Mar-2023
Version number35