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Cellosaurus eCAS (CVCL_7189)

[Text version]
Cell line name eCAS
Synonyms e-CAS
Accession CVCL_7189
Resource Identification Initiative To cite this cell line use: eCAS (RRID:CVCL_7189)
Comments Problematic cell line: Misidentified/contaminated. Originally thought to be of horse origin but found to be a mouse RAW 264.7 derivative (PubMed=29844485). Originally thought to be established by telomerase transfection of bone marrow cells from two 10 year old horses.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00601.
Doubling time: 9.7 hours (PubMed=14700538).
Miscellaneous: Conversion of STR profile from fragment lengths to alleles value by personal communication of Wigger G. (Microsynth).
Derived from site: In situ; Ascites; UBERON=UBERON_0007795.
Cell type: Macrophage; CL=CL_0000235.
Disease Mouse leukemia (NCIt: C21604)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: BALB/c.
Hierarchy Parent: CVCL_0493 (RAW 264.7)
Sex of cell Male
Age at sampling Adult
Category Cancer cell line
STR profile Source(s): PubMed=29844485

Mouse STR 4-222.3,23.3
Mouse STR 5-514
Mouse STR 6-418
Mouse STR 6-712
Mouse STR 12-116,17
Mouse STR 15-322.3
Mouse STR 18-318
Mouse STR X-125

Run an STR similarity search on this cell line
Web pages https://iclac.org/wp-content/uploads/Cross-Contaminations_v12_distribution.xlsx

PubMed=14700538; DOI=10.1016/j.vetimm.2003.08.012
Werners A.H., Bull S., Fink-Gremmels J., Bryant C.E.
Generation and characterisation of an equine macrophage cell line (e-CAS cells) derived from equine bone marrow cells.
Vet. Immunol. Immunopathol. 97:65-76(2004)

PubMed=17686531; DOI=10.1016/j.vetimm.2007.06.027
Steinbach F., Saunders K., Kydd J.H., Ibrahim S.
Further characterization of cross-reactive anti-human leukocyte mAbs by use of equine leukocyte cell lines EqT8888 and eCAS.
Vet. Immunol. Immunopathol. 119:100-105(2007)

PubMed=29844485; DOI=10.1038/s41598-018-26512-3
Evans E., Paillot R., Lopez-Alvarez M.R.
A comprehensive analysis of e-CAS cell line reveals they are mouse macrophages.
Sci. Rep. 8:8237-8237(2018)

Encyclopedic resources Wikidata; Q54831920
Entry history
Entry creation04-Apr-2012
Last entry update29-Jun-2023
Version number14