Cellosaurus cell line hTERT-RPE1 (CVCL

Cellosaurus hTERT-RPE1 (CVCL_4388)

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Cell line name

hTERT-RPE1

Synonyms

hTERT RPE1; hTERT-RPE-1; hTERT RPE-1; hTERT-RPE; TERT-RPE1; RPE-1; RPE1; RPE1-hTERT; RPE1-hTert; RPE-hTERT

Accession

CVCL_4388

Resource Identification Initiative

To cite this cell line use: hTERT-RPE1 (RRID:CVCL_4388)

Comments

Genetic integration: Method=Transfection; Gene=HGNC; HGNC:11730; TERT.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Omics: Phenotyping; CRISPR screening.
Omics: Proteomics.
Omics: Transcriptomics; RNAseq.
Misspelling: hTERT1-RPE1; Note=Occasionally.
Derived from site: In situ; Eye, retina, retinal pigment epithelium; UBERON=UBERON_0001782.
Cell type: Retinal pigment epithelial cell; CL=CL_0002586.

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_B365 (RPE-340)
Children:

CVCL_VP32 (hTERT-RPE1 FRT/TR)	CVCL_E3VG (hTERT-RPE1 PBRM1 KO 1)	CVCL_E3VH (hTERT-RPE1 PBRM1 KO 2)
CVCL_E3VI (hTERT-RPE1 PBRM1 KO 3)	CVCL_E3WG (hTERT-RPE1 SMARCA4 KO 1C9)	CVCL_E3WH (hTERT-RPE1 SMARCA4 KO 1D2)
CVCL_E3WI (hTERT-RPE1 SMARCA4 KO 1H1)	CVCL_E3WJ (hTERT-RPE1 SMARCA4 KO 2BS)	CVCL_E3WK (hTERT-RPE1 SMARCA4 KO TH12)
CVCL_C1AM (RPE1_APC_836)	CVCL_C1AN (RPE1_APC_845)	CVCL_C1AP (RPE1_ARID1A_1.C7)
CVCL_C1AQ (RPE1_ARID1A_D5)	CVCL_C1AR (RPE1_ATM_303)	CVCL_C1AS (RPE1_ATM_306)
CVCL_C1AT (RPE1_CDKN2A_7)	CVCL_C1AU (RPE1_CDKN2C_241)	CVCL_C1AV (RPE1_CDKN2C_242)
CVCL_C1AW (RPE1_EZH2_2)	CVCL_C1AX (RPE1_EZH2_A1)	CVCL_C1AY (RPE1_EZH2_A4)
CVCL_C1CL (RPE1_HPRT1_E10)	CVCL_C1AZ (RPE1_MLL2_8)	CVCL_C1B0 (RPE1_MSH2_393)
CVCL_C1B1 (RPE1_MSH2_394)	CVCL_C1B2 (RPE1_MSH2_405)	CVCL_C1B3 (RPE1_MSH6_266)
CVCL_C1B4 (RPE1_MSH6_269)	CVCL_C1B5 (RPE1_MSH6_271)	CVCL_C1CG (RPE1_MTAP_5)
CVCL_C1CH (RPE1_MTAP_6)	CVCL_C1CI (RPE1_MTAP_7)	CVCL_C1B6 (RPE1_NOTCH1_2)
CVCL_C1B7 (RPE1_NOTCH1_B6)	CVCL_C1B8 (RPE1_NOTCH1_C2)	CVCL_C1B9 (RPE1_PTCH1_3)
CVCL_C1BA (RPE1_PTCH1_6)	CVCL_C1BB (RPE1_PTEN_2)	CVCL_C1BC (RPE1_PTEN_8)
CVCL_C1BD (RPE1_STAG2_2)	CVCL_C1BE (RPE1_STAG2_5)	CVCL_C1BF (RPE1_STAG2_8)
CVCL_C1BG (RPE1_TET2_C9)	CVCL_C1BH (RPE1_TET2_D2)	CVCL_C1BI (RPE1_TET2_E7)
CVCL_C1BJ (RPE1_TP53_219)	CVCL_C1BK (RPE1_TP53_221)	CVCL_C1BL (RPE1_TP53_224)
CVCL_C8DM (RPE1-SS111)	CVCL_C8DN (RPE1-SS119)	CVCL_C8DP (RPE1-SS31)
CVCL_C8DQ (RPE1-SS48)	CVCL_C8DR (RPE1-SS51)	CVCL_C8DS (RPE1-SS6)
CVCL_C8DT (RPE1-SS77)

Sex of cell

Female

Age at sampling

Category

Telomerase immortalized cell line

STR profile

Source(s): ATCC=CRL-4000; PubMed=25877200

Markers:

Amelogenin	X
CSF1PO	12,14
D2S1338	19,24
D3S1358	15,16
D5S818	11
D7S820	10,11
D8S1179	10
D13S317	11,12
D16S539	11
D18S51	14,16
D19S433	14,16.2
D21S11	31,32.2
FGA	20,23
Penta D	9,11
Penta E	12,14
TH01	9
TPOX	8
vWA	17,18

Web pages

Info; CIL; 35201; https://www.cellimagelibrary.org/images/35201
Info; HPA; -; https://www.proteinatlas.org/learn/cellines

Publications

PubMed=9454332; DOI=10.1126/science.279.5349.349
Andrea G. Bodnar, Michel M. Ouellette, Maria Frolkis, Shawn E. Holt, Choy-Pik Chiu, Gregg B. Morin, Calvin B. Harley, Jerry W. Shay, Serge Patrick Lichtsteiner, Woodring Erik Wright;
Extension of life-span by introduction of telomerase into normal human cells.
Science 279:349-352(1998)

PubMed=11980883
Lakshmi Rambhatla, Choy-Pik Chiu, Randolph D. Glickman, Cheryl Rowe-Rendleman;
In vitro differentiation capacity of telomerase immortalized human RPE cells.
Invest. Ophthalmol. Vis. Sci. 43:1622-1630(2002)

PubMed=25177495; DOI=10.1155/2014/801787; PMCID=PMC4142280
Alla V. Kuznetsova, Alexander M. Kurinov, Maria A. Aleksandrova;
Cell models to study regulation of cell transformation in pathologies of retinal pigment epithelium.
J. Ophthalmol. 2014:801787.1-801787.18(2014)

PubMed=25877200; DOI=10.1038/nature14397
Ma-Mie Yu, Suresh K. Selvaraj, May M.Y. Liang-Chu, Sahar Aghajani, Matthew Busse, Jean Yuan, Genee Lee, Franklin V. Peale, Christiaan Klijn, Richard Bourgon, Joshua S. Kaminker, Richard M. Neve;
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=28350380; DOI=10.1038/sdata.2017.33; PMCID=PMC5369315
Jo Lynne Harenza, Maura A. Diamond, Rebecca N. Adams, Michael M. Song, Heather L. Davidson, Lori S. Hart, Maiah H. Dent, Paolo Fortina, Charles Patrick Reynolds, John Matthew Maris;
Transcriptomic profiling of 39 commonly-used neuroblastoma cell lines.
Sci. Data 4:170033.1-170033.8(2017)

PubMed=30971826; DOI=10.1038/s41586-019-1103-9
Fiona M. Behan, Francesco Iorio, Gabriele Picco, Emanuel Goncalves, Charlotte M. Beaver, Giorgia Migliardi, Rita Santos, Yanhua Rao, Francesco Sassi, Marika Pinnelli, Rizwan Ansari ...Show all 25 authors... , Sarah Harper, David Adam Jackson, Rebecca McRae, Rachel Pooley, Piers Wilkinson, Dieudonne J. van der Meer, David Dow, Carolyn A. Buser-Doepner, Andrea Bertotti, Livio Trusolino, Euan Alexander Stronach, Julio Saez-Rodriguez, Kosuke Yusa, Mathew J. Garnett; Show fewer authors
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature 568:511-516(2019)

Cross-references

Cell line collections (Providers)

ATCC; CRL-4000

Cell line databases/resources

Cell_Model_Passport; SIDM00409

Anatomy/cell type resources

BTO; BTO_0004790

Biological sample resources

4DN; 4DNSRHGVFSRJ
BioSample; SAMN03472089

CRISP screens repositories

BioGRID_ORCS_Cell_line; 157

Chemistry resources