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Cellosaurus NCTC clone 929 (CVCL_0462)

[Text version]
Cell line name NCTC clone 929
Synonyms NCTC 929; NCTC-929; NCTC929; NCTC-929L; L cell; L cells; L-cell; L-cells; L cell line; L; Strain L-929; L-929; L 929; L929; L929(NCTC); Clone 929; Earles's cells; Earle's L cells
Accession CVCL_0462
Resource Identification Initiative To cite this cell line use: NCTC clone 929 (RRID:CVCL_0462)
Comments Group: Space-flown cell line (cellonaut).
Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).
Virology: Not susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257).
Doubling time: 28 hours (Note=From cell counting), 32 hours (Note=From absorbance) (DOI=10.5897/IJBMBR2013.0154); ~25 hours (CLS=400260); ~21-24 hours (DSMZ=ACC-2).
Omics: Proteomics; Quantitative.
Anecdotal: First established continuous cell line.
Miscellaneous: PubMed=23430347 has a different value for STR 6-4 (16) than that of NIST (17,18) due to a change in the marker motif (personal communication of Almeida, Jamie L.).
Derived from site: In situ; Connective tissue; UBERON=UBERON_0002384.
Cell type: Fibroblast; CL=CL_0000057.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C3H/An.
Hierarchy Children:
CVCL_DD99 (1R [Mouse L cells])CVCL_3984 (A-9)CVCL_4114 (B82)
CVCL_8887 (L Cells (TK+, HBS Ag+))CVCL_IW04 (L-929 D)CVCL_IW05 (L-929 E)
CVCL_IW06 (L-929 F)CVCL_IW07 (L-929 HH)CVCL_4535 (L-M)
CVCL_U990 (L.P3)CVCL_0D72 (L4.5)CVCL_AR58 (L929/A)
CVCL_E2Z7 (L929/CD275)CVCL_C5ES (L929/CD83)CVCL_4237 (L929/R)
CVCL_4238 (L929S)CVCL_4Z39 (LA-9)CVCL_B950 (LAG)
CVCL_WY44 (LMCAT)CVCL_E7GZ (LS1)CVCL_E7H0 (LS2 [Mouse fibroblast])
CVCL_E7H1 (LS3)CVCL_E7H2 (LS4)CVCL_E7H3 (LS5)
CVCL_E7H4 (LS6)CVCL_E7H5 (LS7)CVCL_3742 (McCoy)
CVCL_4255 (NCTC 2071)CVCL_A0ZP (NCTC 2168)CVCL_C8QV (Wnt-LIGHT)
Sex of cell Male
Age at sampling 100D
Category Spontaneously immortalized cell line
STR profile Source(s): DSMZ=ACC-2; PubMed=23430347; PubMed=31220119; Ubigene=YC-C091

Markers:
Mouse STR 1-110
Mouse STR 1-217
Mouse STR 2-19
Mouse STR 3-213,14
Mouse STR 4-220.3
Mouse STR 5-514
Mouse STR 6-416,17 (DSMZ=ACC-2)
17,18 (PubMed=23430347; PubMed=31220119; Ubigene=YC-C091)
Mouse STR 6-712
Mouse STR 7-125,26,27
Mouse STR 8-116
Mouse STR 9-215
Mouse STR 11-215,16
Mouse STR 12-116
Mouse STR 13-117
Mouse STR 15-324.3,25.3,26.3
Mouse STR 17-215
Mouse STR 18-316
Mouse STR 19-212
Mouse STR X-126,27

Run an STR similarity search on this cell line
Web pages Info; COPE; -; http://www.cells-talk.com/index.php/page/copelibrary?key=L929
Info; ResearchGate; -; https://www.researchgate.net/post/Is_L929_normal_or_cancer_cell_line
Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/l/cell-lines-detail-125.html
Provider; Vircell; -; https://www.vircell.com/en/products/l-929-cell-line
Publications

PubMed=18105872; DOI=10.1093/jnci/9.3.229
Katherine Koontz Sanford, Wilton Robinson Earle, Gwendolyn Likely Hobbs;
The growth in vitro of single isolated tissue cells.
J. Natl. Cancer Inst. 9:229-246(1948)

PubMed=13293658
Katherine Koontz Sanford, Gwendolyn Likely Hobbs, Wilton Robinson Earle;
The tumor-producing capacity of strain L mouse cells after 10 years in vitro.
Cancer Res. 16:162-166(1956)

PubMed=13431913; DOI=10.3181/00379727-94-23044
George E. Foley, Alfred H. Handler;
Differentiation of normal and neoplastic cells maintained in tissue culture by implantation into normal hamsters.
Proc. Soc. Exp. Biol. Med. 94:661-664(1957)

PubMed=13587550; DOI=10.1083/jcb.4.5.567; PMCID=PMC2224560
Benton Bosworth Westfall, Ellison V. Peppers, Virginia John Evans, Katherine Koontz Sanford, Naomi M. Hawkins, Mary C. Fioramonti, Harold A. Kerr, Gwendolyn Likely Hobbs, Wilton Robinson Earle;
The arginase and rhodanese activities of certain cell strains after long cultivation in vitro.
J. Biophys. Biochem. Cytol. 4:567-570(1958)

PubMed=14185313; DOI=10.1126/science.146.3641.241
Cell Culture Collection Committee
Animal cell strains. The Cell Culture Collection Committee has assembled and certified 23 strains of animal cells.
Science 146:241-243(1964)

DOI=10.1007/BF02618370
Cyril S. Stulberg, Lewis Lemon Coriell, Alexis John Kniazeff, John E. Shannon;
The animal cell culture collection.
In Vitro 5:1-16(1970)

PubMed=4328993; DOI=10.1016/0014-4827(71)90412-5
Toshiko Takaoka, Hajim Katsuta;
Long-term cultivation of mammalian cell strains in protein- and lipid-free chemically defined synthetic media.
Exp. Cell Res. 67:295-304(1971)

PubMed=4560675
Kazuko Tsuboi, Toshiko Takaoka, Hajim Katsuta;
Comparative analyses of strain L-929 and its substrain L-P3 grown in a protein- and lipid-free synthetic medium.
Jpn. J. Exp. Med. 42:361-376(1972)

PubMed=4471172; DOI=10.1007/BF02615345
Richard G. Ham;
Nutritional requirements of primary cultures. A neglected problem of modern biology.
In Vitro 10:119-129(1974)

PubMed=6298990; DOI=10.1016/0378-1135(82)90056-6
Dale A. McPhee, Ian Malcolm Parsonson, Anthony James Della-Porta;
Comparative studies on the growth of Australian bluetongue virus serotypes in continuous cell lines and embryonated chicken eggs.
Vet. Microbiol. 7:401-410(1982)

DOI=10.4051/ibc.2010.2.4.0014
Kaori Yoshino, Kaoru Saijo, Chikako Noro, Yukio Nakamura;
Development of a simple method to determine the mouse strain from which cultured cell lines originated.
Interdisciplinary Bio Cent. 2:14.1-14.9(2010)

DOI=10.5897/IJBMBR2013.0154
Simon Bernard Iloki Assanga, Armida Andrea Gil-Salido, Lydianis Maria Lewis Lujan, Aaron de Jesus Rosas-Durazo, Ana Lilian Acosta-Silva, Elba Griselda Rivera-Castaneda, Jose Luis Rubio-Pino;
Cell growth curves for different cell lines and their relationship with biological activities.
Int. J. Biotechnol. Mol. Biol. Res. 4:60-70(2013)

PubMed=23430347; DOI=10.1007/s10616-013-9545-7; PMCID=PMC3886540
Jamie L. Almeida, Carolyn R. Hill, Kenneth D. Cole;
Mouse cell line authentication.
Cytotechnology 66:133-147(2014)

PubMed=31220119; DOI=10.1371/journal.pone.0218412; PMCID=PMC6586308
Jamie L. Almeida, Aleksandra Dakic, Karin Kindig, Maikan Kone, Deborah Lee Dormady Letham, Scott Langdon, Ruth Peat, Jayamalini Holding-Pillai, Erin M. Hall, Mark Ladd, Megan D. Shaffer ...Show all 23 authors... , Heath Berg, Jin-Liang Li, Georges Wigger, Steve Lund, Carolyn R. Steffen, Barbara B. Fransway, Bob Geraghty, Manuela Natoli, Beth A. Bauer, Susanne M. Gollin, Dale W. Lewis, Yvonne A. Reid; Show fewer authors
Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.
PLoS ONE 14:e0218412.1-e0218412.24(2019)

PubMed=32218446; DOI=10.1038/s41597-020-0449-z; PMCID=PMC7099061
Chuan-Qi Zhong, Jian-Feng Wu, Xing-Feng Qiu, Xi Chen, Chang-Chuan Xie, Jia-Huai Han;
Generation of a murine SWATH-MS spectral library to quantify more than 11,000 proteins.
Sci. Data 7:104.1-104.9(2020)

PubMed=33389257; DOI=10.1007/s10096-020-04106-0; PMCID=PMC7778494
Nathalie Wurtz, Gwilherm Penant, Priscilla Jardot, Nathalie Duclos, Bernard La Scola;
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)

PubMed=34737324; DOI=10.1038/s41598-021-00779-5; PMCID=PMC8569163
Oliver Lung, Rebecca C. Candlish, Michelle Nebroski, Peter Kruckiewicz, Cody Buchanan, Mariko Moniwa;
High-throughput sequencing for species authentication and contamination detection of 63 cell lines.
Sci. Rep. 11:21657.1-21657.9(2021)

Cross-references
Cell line collections (Providers) AddexBio; P0011012/5025
ATCC; CCL-1
ATCC; CRL-6364 - Discontinued
BCRC; 60091
BCRJ; 0188
CCLV; CCLV-RIE 0014
CCLV; CCLV-RIE 0287
CCTCC; GDC0034
CLS; 400260
DSMZ; ACC-2
ECACC; 85011425
ECACC; 85103115
ECACC; 88102702
IBRC; C10102
ICLC; ATL95001
IZSLER; BS CL 56
JCRB; IFO50409
JCRB; JCRB9003
KCB; KCB 92012YJ
KCLB; 10001
NCBI_Iran; C161
RCB; RCB0081 - Discontinued
RCB; RCB1422
RCB; RCB1451
RCB; RCB2619
TKG; TKG 0217
Ubigene; YC-C091
Cell line databases/resources CLO; CLO_0007144
CLO; CLO_0007219
CLO; CLO_0007220
CLO; CLO_0007221
CLO; CLO_0008140
CLO; CLO_0050233
CLO; CLO_0050234
CLO; CLO_0050235
MCCL; MCC:0000359
CLDB; cl3154
CLDB; cl3156
CLDB; cl3157
CLDB; cl3158
CLDB; cl3159
CLDB; cl3160
CLDB; cl3673
CLDB; cl3674
CLDB; cl3675
CLDB; cl3676
CCRID; 1101MOU-PUMC000403
CCRID; 1102MOU-NIFDC00019
CCRID; 3101MOUGNM28
CCRID; 3101MOUSCSP5039
CCRID; 4201MOU-CCTCC00034
CCRID; 5301MOU-KCB92012YJ
DSMZCellDive; ACC-2
FCS-free; 8-5-530-1-3-3
FCS-free; 8-5-9-1-3-3
Lonza; 32
Lonza; 937
TOKU-E; 3695
Anatomy/cell type resources BTO; BTO_0000331
Biological sample resources BioSample; SAMN11397622
CRISP screens repositories BioGRID_ORCS_Cell_line; 1749
Chemistry resources ChEMBL-Cells; CHEMBL3307946
ChEMBL-Targets; CHEMBL612266
ChEMBL-Targets; CHEMBL612267
PubChem_Cell_line; CVCL_0462
Encyclopedic resources Wikidata; Q28335098
Medical resources MeSH; D007739
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number43