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Cellosaurus MDCK-II (CVCL_0424)

[Text version]
Cell line name MDCK-II
Synonyms MDCK II; MDCKII; MDCK2; MDCK-2; MDCK Type II; MDCKII-WT
Accession CVCL_0424
Resource Identification Initiative To cite this cell line use: MDCK-II (RRID:CVCL_0424)
Comments Group: Vaccine production cell line.
Omics: Proteomics.
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Cell type: Epithelial cell of kidney; CL=CL_0002518.
Species of origin Canis lupus familiaris (Dog) (Canis familiaris) (NCBI Taxonomy: 9615)
Breed/subspecies: Cocker Spaniel; VBO=VBO_0200372.
Hierarchy Parent: CVCL_0422 (MDCK)
Children:
CVCL_E7Z3 (MDCK II CLDN2 KO1)CVCL_E7Z4 (MDCK II CLDN2 KO2)CVCL_E7Z5 (MDCK II CLDN2 KO3)
CVCL_E7Z6 (MDCK II CLDN2 KO4)CVCL_E7Z7 (MDCK II claudin-2 knockout clone 5)CVCL_E7Z8 (MDCK II CLDN4 KO1)
CVCL_E7Z9 (MDCK II CLDN4 KO2)CVCL_E7ZA (MDCK II CLDN4 KO3)CVCL_WY48 (MDCK-17)
CVCL_ZW00 (MDCK-A3-10)CVCL_ZW01 (MDCK-A3-14)CVCL_ZV47 (MDCK-A3-15)
CVCL_B3SF (MDCK-II Abcc1)CVCL_A6UX (MDCK-II Rab10-KO)CVCL_A6UY (MDCK-II Rab11-KO)
CVCL_A6VA (MDCK-II Rab12-KO)CVCL_A6VB (MDCK-II Rab13-KO)CVCL_A6VC (MDCK-II Rab14-KO)
CVCL_A6VD (MDCK-II Rab15-KO)CVCL_A6VE (MDCK-II Rab17-KO)CVCL_A6VF (MDCK-II Rab18-KO)
CVCL_A6VG (MDCK-II Rab19-KO)CVCL_A6VI (MDCK-II Rab1A-KO)CVCL_A6VJ (MDCK-II Rab1B-KO)
CVCL_A6VK (MDCK-II Rab2-KO)CVCL_A6VL (MDCK-II Rab20-KO)CVCL_A6VM (MDCK-II Rab21-KO)
CVCL_A6VN (MDCK-II Rab22-KO)CVCL_A6VP (MDCK-II Rab23-KO)CVCL_A6VQ (MDCK-II Rab24-KO)
CVCL_A6VR (MDCK-II Rab25-KO)CVCL_A6VS (MDCK-II Rab26-KO)CVCL_A6VU (MDCK-II Rab27-KO)
CVCL_A6VV (MDCK-II Rab28-KO)CVCL_A6VW (MDCK-II Rab29-KO)CVCL_A6VX (MDCK-II Rab3-KO)
CVCL_A6VY (MDCK-II Rab30-KO)CVCL_A6VZ (MDCK-II Rab32/38-KO)CVCL_A6WA (MDCK-II Rab33-KO)
CVCL_A6WB (MDCK-II Rab34-KO)CVCL_A6WD (MDCK-II Rab35-KO)CVCL_A6WE (MDCK-II Rab36-KO)
CVCL_A6WF (MDCK-II Rab37-KO)CVCL_A6WG (MDCK-II Rab39-KO)CVCL_A6WI (MDCK-II Rab4-KO)
CVCL_A6WJ (MDCK-II Rab40-KO)CVCL_A6WK (MDCK-II Rab42-KO)CVCL_A6WL (MDCK-II Rab43-KO)
CVCL_A6WM (MDCK-II Rab5AB-KO)CVCL_A6WN (MDCK-II Rab5C-KO)CVCL_A6WP (MDCK-II Rab6-KO)
CVCL_A6WQ (MDCK-II Rab7A-KO)CVCL_A6WV (MDCK-II Rab7AB-KO)CVCL_A6WR (MDCK-II Rab7B-KO)
CVCL_A6WS (MDCK-II Rab8-KO)CVCL_A6WT (MDCK-II Rab9-KO)CVCL_S586 (MDCK-MDR1)
CVCL_X022 (MDCK-MRP2)CVCL_A2JZ (MDCK2-ABCB1)CVCL_A2JY (MDCK2-ABCC2)
CVCL_E6JH (MDCKII ABCA1 HA-KM)CVCL_E6JI (MDCKII ABCA1 HA-MK)CVCL_E6JJ (MDCKII ABCA1 HA-MM)
CVCL_E6JK (MDCKII ABCA1 HA-WT)CVCL_C3F0 (MDCKII MDR1 KO)CVCL_VR40 (MDCKII-LE)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=1468552; DOI=10.1016/0014-5793(92)81476-3
Jurgen Grunberg, Ursula Luginbuhl, Erwin E. Sterchi;
Proteolytic processing of human intestinal lactase-phlorizin hydrolase precursor is not a prerequisite for correct sorting in Madin Darby canine kidney (MDCK) cells.
FEBS Lett. 314:224-228(1992)

PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Axel Karger, Barbara Bettin, Matthias Lenk, Thomas Christoph Mettenleiter;
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)

PubMed=21766308; DOI=10.1002/jps.22674
Li Di, Carrie Whitney-Pickett, John P. Umland, Hui Zhang, Xun Zhang, David F. Gebhard, Yu-Rong Lai, James J. Federico 3rd, Ralph E. Davidson, Russ Smith, Eric L. Reyner ...Show all 20 authors... , Caroline Lee, Bo Feng, Charles Rotter, Manthena V. Varma, Sarah Kempshall, Katherine Fenner, Ayman F. El-Kattan, Theodore E. Liston, Matthew D. Troutman; Show fewer authors
Development of a new permeability assay using low-efflux MDCKII cells.
J. Pharm. Sci. 100:4974-4985(2011)

PubMed=21982418; DOI=10.1186/1471-2121-12-43; PMCID=PMC3209442
Joseph D. Dukes, Paul Whitley, Andrew D. Chalmers;
The MDCK variety pack: choosing the right strain.
BMC Cell Biol. 12:43.1-43.4(2011)

PubMed=24975811; DOI=10.1016/j.vaccine.2014.06.045; PMCID=PMC5915289
Ruben O. Donis, I-Mei Chen, C. Todd Davis, Angie Foust, M. Jaber Hossain, Adam Johnson, Alexander Klimov, Rosette F. Loughlin, Xi-Yan Xu, Theodore Tsai, Simone Blayer ...Show all 24 authors... , Heidi Trusheim, Tony Colegate, John Fox, Beverly Taylor, Althaf I. Hussain, Ian Barr, Chantal Baas, Jaap Louwerens, Ed Geuns, Min-Shi Lee, Lodewijk Venhuizen, Elisabeth Neumeier, Thedi Ziegler; Show fewer authors
Influenza Cell Culture Working Group
Performance characteristics of qualified cell lines for isolation and propagation of influenza viruses for vaccine manufacturing.
Vaccine 32:6583-6590(2014)

PubMed=34737324; DOI=10.1038/s41598-021-00779-5; PMCID=PMC8569163
Oliver Lung, Rebecca C. Candlish, Michelle Nebroski, Peter Kruckiewicz, Cody Buchanan, Mariko Moniwa;
High-throughput sequencing for species authentication and contamination detection of 63 cell lines.
Sci. Rep. 11:21657.1-21657.9(2021)

Cross-references
Cell line collections (Providers) AddexBio; P0014001/82
CCLV; CCLV-RIE 1061
CLS; 305233
ECACC; 00062107
RCB; RCB5148
Cell line databases/resources MCCL; MCC:0000321
Lonza; 811
Anatomy/cell type resources BTO; BTO_0004607
Chemistry resources ChEMBL-Cells; CHEMBL3308862
ChEMBL-Targets; CHEMBL4296466
PubChem_Cell_line; CVCL_0424
Encyclopedic resources Wikidata; Q54904781
Proteomic databases PRIDE; PXD001130
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number24