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Cellosaurus MDCK (CVCL_0422)

[Text version]
Cell line name MDCK
Synonyms MDCK (NBL-2); MDCK(NBL-2); NBL-2; Madin-Darby Canine Kidney; Madin Darby Canine Kidney
Accession CVCL_0422
Resource Identification Initiative To cite this cell line use: MDCK (RRID:CVCL_0422)
Comments Group: Vaccine production cell line.
Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).
Characteristics: Used for virus production for vaccine development, toxicology and industrial biotechnology research, and high-throughput screening (ATCC=CCL-34).
Virology: Not susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257).
Omics: Transcriptomics; RNAseq.
Caution: There seem to be two distinct cell lines which were assigned NCBI_Iran catalog number C426.
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Cell type: Epithelial cell of kidney; CL=CL_0002518.
Species of origin Canis lupus familiaris (Dog) (Canis familiaris) (NCBI Taxonomy: 9615)
Breed/subspecies: Cocker Spaniel; VBO=VBO_0200372.
Hierarchy Children:
CVCL_4164 (DoCl1 (S+L-))CVCL_WL43 (hCK)CVCL_YJ87 (LINTERNA MDCK)
CVCL_GY95 (Luc9.1)CVCL_2A36 (MDCK 33016)CVCL_DF69 (MDCK 9B9-1B1)
CVCL_DF70 (MDCK 9B9-1E4)CVCL_ZV91 (MDCK Actin-cpstFRET-Actin)CVCL_ZV92 (MDCK Actinin-C-cpstFRET)
CVCL_ZV93 (MDCK Actinin-M-cpstFRET)CVCL_D5IF (MDCK BLVRA KO)CVCL_C8RJ (MDCK ConAr)
CVCL_ZV90 (MDCK cpstFRET-Actin)CVCL_C8RK (MDCK RCAr)CVCL_D3W3 (MDCK siat7e-expressing clone 1)
CVCL_D3W4 (MDCK siat7e-expressing clone 2)CVCL_AP67 (MDCK Sky1023)CVCL_AP68 (MDCK Sky10234)
CVCL_AP69 (MDCK Sky3851)CVCL_V359 (MDCK Tet-Off)CVCL_B5WN (MDCK tGFP-APP)
CVCL_B0N7 (MDCK-15D3)CVCL_B4IA (MDCK-2B2)CVCL_WL59 (MDCK-6A8)
CVCL_K177 (MDCK-AD)CVCL_AP66 (MDCK-B-702)CVCL_A2JC (MDCK-C09)
CVCL_AP70 (MDCK-C11)CVCL_A2JD (MDCK-C35)CVCL_0423 (MDCK-C7)
CVCL_DF31 (MDCK-chAbcb1)CVCL_D5I6 (MDCK-dADAM17)CVCL_D5HY (MDCK-dEGF_#1)
CVCL_D5I7 (MDCK-dEGF_#2)CVCL_D5I5 (MDCK-dEGF/HBEGF/TGFalpha/EREG KO 1)CVCL_D5HZ (MDCK-dEREG)
CVCL_D5I0 (MDCK-dHBEGF)CVCL_D5I1 (MDCK-dTGFalpha)CVCL_AQ31 (MDCK-E [Korea])
CVCL_Y089 (MDCK-E [Taiwan])CVCL_D5IG (MDCK-EKAREV-NLS)CVCL_D5IR (MDCK-EKARrEV-NLS)
CVCL_AP75 (MDCK-F)CVCL_0592 (MDCK-I)CVCL_0424 (MDCK-II)
CVCL_IZ19 (MDCK-P-gp)CVCL_JX35 (MDCK-Protein Free)CVCL_D3UF (MDCK-RE7)
CVCL_IQ72 (MDCK-S)CVCL_IQ73 (MDCK-SF101)CVCL_IQ74 (MDCK-SF102)
CVCL_IQ75 (MDCK-SF103)CVCL_WL74 (MDCK-SFS)CVCL_Z936 (MDCK-SIAT1)
CVCL_A6IW (MDCK-UT-A1)CVCL_A2JE (MDCK-W73)CVCL_A9IZ (MDCK-XF02)
CVCL_A9JA (MDCK-XF03)CVCL_A9JB (MDCK-XF04)CVCL_B033 (MDCK.1)
CVCL_B034 (MDCK.2)CVCL_DE56 (MDCK.5F1)CVCL_2586 (MDCK.P3)
CVCL_YZ44 (MDCK.STAT1 KO)CVCL_WL70 (MDCK.SUS1)CVCL_K241 (MDCK/60)
CVCL_WC98 (MDCK/IgR)CVCL_WL76 (ssf-MDCK)CVCL_6452 (Super Dome)
CVCL_6453 (Super Tube)CVCL_E0YT (Ubigene MDCK ANP32A KO)CVCL_E0YU (Ubigene MDCK CADM1 KO)
CVCL_E0YV (Ubigene MDCK GSR KO)CVCL_E0YW (Ubigene MDCK JAM2 KO)CVCL_E0YX (Ubigene MDCK JAM3 KO)
CVCL_E0YY (Ubigene MDCK L1CAM KO)CVCL_E0YZ (Ubigene MDCK MAS1 KO)CVCL_E0Z0 (Ubigene MDCK NPHP1 KO)
CVCL_E0Z1 (Ubigene MDCK STAT1 KO)CVCL_E0Z2 (Ubigene MDCK TGM2 KO)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Web pages Info; -; -; https://micro.magnet.fsu.edu/primer/techniques/fluorescence/gallery/cells/mdck/mdckcells.html
Info; ATCC; -; https://www.atcc.org/en/support/technical-support/faqs/morphology-of-atcc-ccl-34-mdck
Info; ECACC; -; https://www.culturecollections.org.uk/products/cell-cultures/ecacc-cell-line-profiles/mdck/
Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/m/cell-lines-detail-89.html
Info; Wikipedia; -; https://en.wikipedia.org/wiki/Madin-Darby_Canine_Kidney_Cells
Provider; Vircell; -; https://www.vircell.com/en/products/mdck-cell-line
Publications

PubMed=13901412; DOI=10.1126/science.138.3536.42
Irving J. Green;
Serial propagation of influenza B (Lee) virus in a transmissible line of canine kidney cells.
Science 138:42-43(1962)

PubMed=5918973; DOI=10.3181/00379727-122-31293
Charles R. Gaush, Walter L. Hard, Thomas F. Smith;
Characterization of an established line of canine kidney cells (MDCK).
Proc. Soc. Exp. Biol. Med. 122:931-935(1966)

DOI=10.1007/BF02618370
Cyril S. Stulberg, Lewis Lemon Coriell, Alexis John Kniazeff, John E. Shannon;
The animal cell culture collection.
In Vitro 5:1-16(1970)

PubMed=222773; DOI=10.1083/jcb.81.3.635; PMCID=PMC2110404
Michael J. Rindler, Lorraine M. Chuman, Leilani Shaffer, Milton H. Saier Jr.;
Retention of differentiated properties in an established dog kidney epithelial cell line (MDCK).
J. Cell Biol. 81:635-648(1979)

PubMed=6480288
Wilfried Schroyens, Remy Bruyneel, Ruy Tchao, Joseph Leighton, Christian H. Dragonetti, Marc M. Mareel;
Comparison of invasiveness and non-invasiveness of two epithelial cell lines in vitro.
Invasion Metastasis 4:160-170(1984)

PubMed=12667817; DOI=10.1016/S0042-6822(02)00064-8
Julia Romanova, Dietmar Katinger, Boris Ferko, Regina Voglauer, Larisa Mochalova, Nicolai Bovin, Wilina Lim, Hermann Katinger, Andrej Egorov;
Distinct host range of influenza H3N2 virus isolates in Vero and MDCK cells is determined by cell specific glycosylation pattern.
Virology 307:90-97(2003)

PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Axel Karger, Barbara Bettin, Matthias Lenk, Thomas Christoph Mettenleiter;
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)

PubMed=21819694; PMCID=PMC3123757
Romelda L. Omeir, Belete Teferedegne, Gideon S. Foseh, Joel J. Beren, Philip Joseph Snoy, Lauren R. Brinster, James L. Cook, Keith Peden, Andrew M. Lewis Jr.;
Heterogeneity of the tumorigenic phenotype expressed by Madin-Darby canine kidney cells.
Comp. Med. 61:243-250(2011)

PubMed=21982418; DOI=10.1186/1471-2121-12-43; PMCID=PMC3209442
Joseph D. Dukes, Paul Whitley, Andrew D. Chalmers;
The MDCK variety pack: choosing the right strain.
BMC Cell Biol. 12:43.1-43.4(2011)

PubMed=24058646; DOI=10.1371/journal.pone.0075014; PMCID=PMC3772841
Vladimir Y. Lugovtsev, Darya Melnyk, Jerry P. Weir;
Heterogeneity of the MDCK cell line and its applicability for influenza virus research.
PLoS ONE 8:e75014.1-e75014.16(2013)

PubMed=25903999; DOI=10.1002/biot.201400388
Yvonne Genzel;
Designing cell lines for viral vaccine production: where do we stand?
Biotechnol. J. 10:728-740(2015)

PubMed=32854295; DOI=10.3390/ijms21176111; PMCID=PMC7504089
Ana Filipa Rodrigues, Paulo Fernandes, Tanja Laske, Rute Castro, Paula Marques Alves, Yvonne Genzel, Ana Sofia Coroadinha;
Cell bank origin of MDCK parental cells shapes adaptation to serum-free suspension culture and canine adenoviral vector production.
Int. J. Mol. Sci. 21:6111.1-6111.27(2020)

PubMed=33122286; DOI=10.1681/ASN.2020010009; PMCID=PMC7894662
Syed J. Khundmiri, Li-He Chen, Eleanor D. Lederer, Chin-Rang Yang, Mark A. Knepper;
Transcriptomes of major proximal tubule cell culture models.
J. Am. Soc. Nephrol. 32:86-97(2021)

PubMed=33389257; DOI=10.1007/s10096-020-04106-0; PMCID=PMC7778494
Nathalie Wurtz, Gwilherm Penant, Priscilla Jardot, Nathalie Duclos, Bernard La Scola;
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)

Cross-references
Cell line collections (Providers) ABM; T8979
AddexBio; P0014003/4928
ATCC; CCL-34
ATCC; CRL-6253 - Discontinued
BCRC; 60004
BCRJ; 0168
BEI_Resources; NR-2628
CCLV; CCLV-RIE 0083
CCTCC; GDC0012
CCTCC; GDC0401
CLS; 602280
ECACC; 84121903
ECACC; 85011435
IBRC; C10556
IZSLER; BS CL 64
JCRB; IFO50071
JCRB; JCRB9029
KCB; KCB 2006105YJ
KCLB; 10034
NCBI_Iran; C426
RCB; RCB0995
Ubigene; YC-C005
Cell line databases/resources CLO; CLO_0007646
CLO; CLO_0007647
CLO; CLO_0050861
MCCL; MCC:0000319
CLDB; cl3417
CLDB; cl3418
CLDB; cl3419
CLDB; cl3420
CLDB; cl3421
CLDB; cl3422
CLDB; cl3423
CLDB; cl3424
CLDB; cl3425
CCRID; 1101CAN-PUMC000078
CCRID; 1102CAN-NIFDC00045
CCRID; 3101CANGNO23
CCRID; 4201CAN-CCTCC00012
FCS-free; 41-14-321-2-3-3
FCS-free; 41-14-427-1-4-3
FCS-free; 41-14-47-1-8-9
FCS-free; 41-14-585-1-18-2
FCS-free; 41-14-587-1-18-2
FCS-free; 41-14-588-2-18-2
Lonza; 153
TOKU-E; 2403
TOKU-E; 3636
Anatomy/cell type resources BTO; BTO_0000837
Chemistry resources ChEMBL-Cells; CHEMBL3308370
ChEMBL-Targets; CHEMBL614068
PubChem_Cell_line; CVCL_0422
Encyclopedic resources Wikidata; Q28335011
Experimental variables resources EFO; EFO_0022746
Gene expression databases GEO; GSM4009056
GEO; GSM4009057
GEO; GSM4009058
Medical resources MeSH; D061985
Proteomic databases PRIDE; PXD014768
PRIDE; PXD024031
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number41