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Cellosaurus MDCK siat7e-expressing clone 2 (CVCL_D3W4)

[Text version]
Cell line name MDCK siat7e-expressing clone 2
Accession CVCL_D3W4
Resource Identification Initiative To cite this cell line use: MDCK siat7e-expressing clone 2 (RRID:CVCL_D3W4)
Comments Group: Vaccine production cell line.
Characteristics: Grows in suspension (PubMed=19706449).
Virology: Allows efficient influenza virus growth and hemagglutinin (HA) production (PubMed=19706449).
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:19342; ST6GALNAC5.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Cell type: Epithelial cell of kidney; CL=CL_0002518.
Species of origin Canis lupus familiaris (Dog) (Canis familiaris) (NCBI Taxonomy: 9615)
Breed/subspecies: Cocker Spaniel; VBO=VBO_0200372.
Hierarchy Parent: CVCL_0422 (MDCK)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=19706449; DOI=10.1073/pnas.0905912106; PMCID=PMC2728112
Chia Chu, Vladimir Y. Lugovtsev, Hana Golding, Michael J. Betenbaugh, Joseph Shiloach;
Conversion of MDCK cell line to suspension culture by transfecting with human siat7e gene and its application for influenza virus production.
Proc. Natl. Acad. Sci. U.S.A. 106:14802-14807(2009)

Cross-references
Encyclopedic resources Wikidata; Q127382755
Entry history
Entry creation30-Jan-2024
Last entry update14-Aug-2025
Version number4