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Cellosaurus hCK (CVCL_WL43)

[Text version]
Cell line name hCK
Accession CVCL_WL43
Resource Identification Initiative To cite this cell line use: hCK (RRID:CVCL_WL43)
Comments Group: Vaccine production cell line.
Characteristics: Expresses large amounts of alpha-2,6-sialoglycans and small amounts of alpha-2,3-sialoglycans.
Virology: Will be useful for influenza virus research, particularly studies involving human A/H3N2 influenza viruses and possibly for vaccine production.
Knockout cell: Method=CRISPR/Cas9; VGNC; VGNC:46850; ST3GAL1.
Knockout cell: Method=CRISPR/Cas9; VGNC; VGNC:46851; ST3GAL2.
Knockout cell: Method=CRISPR/Cas9; VGNC; VGNC:108234; ST3GAL3.
Knockout cell: Method=CRISPR/Cas9; VGNC; VGNC:46852; ST3GAL4.
Knockout cell: Method=CRISPR/Cas9; VGNC; VGNC:46853; ST3GAL5.
Knockout cell: Method=CRISPR/Cas9; VGNC; VGNC:46854; ST3GAL6.
Knockout cell: Method=CRISPR/Cas9; UniProtKB; F1Q495; ST3GAL2L.
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:10860; ST6GAL1.
Genetic integration: Method=Transfection; Gene=UniProtKB; P33967; B.cereus Bsr.
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Cell type: Epithelial cell of kidney; CL=CL_0002518.
Species of origin Canis lupus familiaris (Dog) (Canis familiaris) (NCBI Taxonomy: 9615)
Breed/subspecies: Cocker Spaniel; VBO=VBO_0200372.
Hierarchy Parent: CVCL_0422 (MDCK)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=31036910; DOI=10.1038/s41564-019-0433-6
Takada K., Kawakami C., Fan S.-F., Chiba S., Zhong G.-X., Gu C.-Y., Shimizu K., Takasaki S., Sakai-Tagawa Y., Lopes T.J.S., Dutta J., Khan Z., Kriti D., van Bakel H., Yamada S., Watanabe T., Imai M., Kawaoka Y.
A humanized MDCK cell line for the efficient isolation and propagation of human influenza viruses.
Nat. Microbiol. 4:1268-1273(2019)

Cross-references
Encyclopedic resources Wikidata; Q93984530
Entry history
Entry creation24-May-2019
Last entry update19-Dec-2024
Version number11