ID   hCK
AC   CVCL_WL43
DR   Wikidata; Q93984530
RX   PubMed=31036910;
CC   Group: Vaccine production cell line.
CC   Characteristics: Expresses large amounts of alpha-2,6-sialoglycans and small amounts of alpha-2,3-sialoglycans.
CC   Virology: Will be useful for influenza virus research, particularly studies involving human A/H3N2 influenza viruses and possibly for vaccine production.
CC   Knockout cell: Method=CRISPR/Cas9; VGNC; VGNC:46850; ST3GAL1.
CC   Knockout cell: Method=CRISPR/Cas9; VGNC; VGNC:46851; ST3GAL2.
CC   Knockout cell: Method=CRISPR/Cas9; VGNC; VGNC:108234; ST3GAL3.
CC   Knockout cell: Method=CRISPR/Cas9; VGNC; VGNC:46852; ST3GAL4.
CC   Knockout cell: Method=CRISPR/Cas9; VGNC; VGNC:46853; ST3GAL5.
CC   Knockout cell: Method=CRISPR/Cas9; VGNC; VGNC:46854; ST3GAL6.
CC   Knockout cell: Method=CRISPR/Cas9; UniProtKB; F1Q495; ST3GAL2L.
CC   Genetic integration: Method=Transfection; Gene=HGNC; HGNC:10860; ST6GAL1.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P33967; Bacillus cereus Bsr.
CC   Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
CC   Cell type: Epithelial cell of kidney; CL=CL_0002518.
CC   Breed/subspecies: Cocker Spaniel; VBO=VBO_0200372.
OX   NCBI_TaxID=9615; ! Canis lupus familiaris (Dog)
HI   CVCL_0422 ! MDCK
SX   Female
AG   Adult
CA   Spontaneously immortalized cell line
DT   Created: 24-05-19; Last updated: 14-08-25; Version: 12
//
RX   PubMed=31036910; DOI=10.1038/s41564-019-0433-6;
RA   Takada, Kosuke
RA   Kawakami, Chiharu
RA   Fan, Shu-Fang
RA   Chiba, Shiho
RA   Zhong, Gong-Xun
RA   Gu, Chun-Yang
RA   Shimizu, Kohei
RA   Takasaki, Sara
RA   Sakai-Tagawa, Yuko
RA   Lopes, Tiago Jose Silva
RA   Dutta, Jayeeta
RA   Khan, Zenab
RA   Kriti, Divya
RA   van Bakel, Harm
RA   Yamada, Shinya
RA   Watanabe, Tokiko
RA   Imai, Masaki
RA   Kawaoka, Yoshihiro
RT   "A humanized MDCK cell line for the efficient isolation and
RT   propagation of human influenza viruses.";
RL   Nat. Microbiol. 4:1268-1273(2019).
//