ID   hCK
AC   CVCL_WL43
DR   Wikidata; Q93984530
RX   PubMed=31036910;
CC   Group: Vaccine production cell line.
CC   Characteristics: Expresses large amounts of alpha-2,6-sialoglycans and small amounts of alpha-2,3-sialoglycans.
CC   Virology: Will be useful for influenza virus research, particularly studies involving human A/H3N2 influenza viruses and possibly for vaccine production.
CC   Knockout cell: Method=CRISPR/Cas9; VGNC; VGNC:46850; ST3GAL1.
CC   Knockout cell: Method=CRISPR/Cas9; VGNC; VGNC:46851; ST3GAL2.
CC   Knockout cell: Method=CRISPR/Cas9; VGNC; VGNC:108234; ST3GAL3.
CC   Knockout cell: Method=CRISPR/Cas9; VGNC; VGNC:46852; ST3GAL4.
CC   Knockout cell: Method=CRISPR/Cas9; VGNC; VGNC:46853; ST3GAL5.
CC   Knockout cell: Method=CRISPR/Cas9; VGNC; VGNC:46854; ST3GAL6.
CC   Knockout cell: Method=CRISPR/Cas9; UniProtKB; F1Q495; ST3GAL2L.
CC   Genetic integration: Method=Transfection; Gene=HGNC; HGNC:10860; ST6GAL1.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P33967; B.cereus Bsr.
CC   Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
CC   Cell type: Epithelial cell of kidney; CL=CL_0002518.
CC   Breed/subspecies: Cocker Spaniel; VBO=VBO_0200372.
OX   NCBI_TaxID=9615; ! Canis lupus familiaris (Dog)
HI   CVCL_0422 ! MDCK
SX   Female
AG   Adult
CA   Spontaneously immortalized cell line
DT   Created: 24-05-19; Last updated: 19-12-24; Version: 11
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RX   PubMed=31036910; DOI=10.1038/s41564-019-0433-6;
RA   Takada K., Kawakami C., Fan S.-F., Chiba S., Zhong G.-X., Gu C.-Y.,
RA   Shimizu K., Takasaki S., Sakai-Tagawa Y., Lopes T.J.S., Dutta J.,
RA   Khan Z., Kriti D., van Bakel H., Yamada S., Watanabe T., Imai M.,
RA   Kawaoka Y.;
RT   "A humanized MDCK cell line for the efficient isolation and
RT   propagation of human influenza viruses.";
RL   Nat. Microbiol. 4:1268-1273(2019).
//