Cellosaurus cell line MDCK-EKARrEV-NLS (CVCL

Cellosaurus MDCK-EKARrEV-NLS (CVCL_D5IR)

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Cell line name

MDCK-EKARrEV-NLS

Synonyms

MDCK-WT-EKARrEV-NLS

Accession

CVCL_D5IR

Resource Identification Initiative

To cite this cell line use: MDCK-EKARrEV-NLS (RRID:CVCL_D5IR)

Comments

Characteristics: Transduced with the FRET biosensor EKARrEV-NLS FRET biosensor EKARrEV-NLS, which consists of a donor fluorescent protein CFP, an ERK substrate peptide derived from RSK1, an optimized linker, a FHA1 phosphate binding domain, an acceptor fluorescent protein YFP and a nuclear localization signal (PubMed=34667080).
Genetic integration: Method=Transduction; Gene=FPbase; ZRLU4; CFP (Note=Cyan fluorescent protein derived from GFP).
Genetic integration: Method=Transduction; Gene=FPbase; 8DNLG; YFP (Note=EYFP, yellow fluorescent protein derived from GFP).
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Cell type: Epithelial cell of kidney; CL=CL_0002518.

Species of origin

Canis lupus familiaris (Dog) (Canis familiaris) (NCBI Taxonomy: 9615)
Breed/subspecies: Cocker Spaniel; VBO=VBO_0200372.

Hierarchy

Parent: CVCL_0422 (MDCK)
Children:

CVCL_D5IW (MDCK-EKARrEV-NLS-dEGFR)

CVCL_D5IZ (MDCK-EKARrEV-NLS-dEREG)

CVCL_D5J1 (MDCK-WT-EKARrEV-NLS-LDR)

Sex of cell

Female

Age at sampling

Adult