Cellosaurus cell line MDCK-WT-EKARrEV-NLS-LDR (CVCL

Cellosaurus MDCK-WT-EKARrEV-NLS-LDR (CVCL_D5J1)

[Text version]

Cell line name

MDCK-WT-EKARrEV-NLS-LDR

Accession

CVCL_D5J1

Resource Identification Initiative

To cite this cell line use: MDCK-WT-EKARrEV-NLS-LDR (RRID:CVCL_D5J1)

Comments

Characteristics: Transduced with the FRET biosensor EKARrEV-NLS FRET biosensor EKARrEV-NLS, which consists of a donor fluorescent protein CFP, an ERK substrate peptide derived from RSK1, an optimized linker, a FHA1 phosphate binding domain, an acceptor fluorescent protein YFP and a nuclear localization signal as well a construct containing a Lyn-targeted (first 11 residues of LYN) rapamycin-binding (FRB) domain from MTOR (LDR) (PubMed=34667080).
Genetic integration: Method=Transduction; Gene=FPbase; ZRLU4; CFP (Note=Cyan fluorescent protein derived from GFP).
Genetic integration: Method=Transduction; Gene=FPbase; 8DNLG; YFP (Note=EYFP, yellow fluorescent protein derived from GFP).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:3942; MTOR (Note=With p.Met1_Arg2018del and p.Gln2114_Trp2549del = FRB).
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Cell type: Epithelial cell of kidney; CL=CL_0002518.

Species of origin

Canis lupus familiaris (Dog) (Canis familiaris) (NCBI Taxonomy: 9615)
Breed/subspecies: Cocker Spaniel; VBO=VBO_0200372.

Hierarchy

Parent: CVCL_D5IR (MDCK-EKARrEV-NLS)
Children:

CVCL_D5JR (MDCK-WT-EKARrEV-NLS-LDR-mRFP-FKBP-mSos1-linkercat)

Sex of cell

Female

Age at sampling

Adult