Cellosaurus MDCK-WT-EKARrEV-NLS-LDR-mRFP-FKBP-mSos1-linkercat (CVCL_D5JR)
| Cell line name | MDCK-WT-EKARrEV-NLS-LDR-mRFP-FKBP-mSos1-linkercat |
|---|---|
| Accession | CVCL_D5JR |
| Resource Identification Initiative | To cite this cell line use: MDCK-WT-EKARrEV-NLS-LDR-mRFP-FKBP-mSos1-linkercat (RRID:CVCL_D5JR) |
| Comments | Characteristics: Transduced with the FRET biosensor EKARrEV-NLS FRET biosensor EKARrEV-NLS, which consists of a donor fluorescent protein CFP, an ERK substrate peptide derived from RSK1, an optimized linker, a FHA1 phosphate binding domain, an acceptor fluorescent protein YFP and a nuclear localization signal as well a construct containing a Lyn-targeted (first 11 residues of LYN) rapamycin-binding (FRB) domain from MTOR (LDR) and a third construct pT2Aneo-mRFP-FKBP-mSos1-linkercat (PubMed=34667080). Genetic integration: Method=Transduction; Gene=FPbase; ZRLU4; CFP (Note=Cyan fluorescent protein derived from GFP). Genetic integration: Method=Transduction; Gene=FPbase; 5YCFA; mRFP1 (Note=Bright red fluorescent protein derived from drFP583 (DsRed)). Genetic integration: Method=Transduction; Gene=FPbase; 8DNLG; YFP (Note=EYFP, yellow fluorescent protein derived from GFP). Genetic integration: Method=Transduction; Gene=HGNC; HGNC:3711; FKBP1A. Genetic integration: Method=Transduction; Gene=HGNC; HGNC:3942; MTOR (Note=With p.Met1_Arg2018del and p.Gln2114_Trp2549del = FRB). Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo. Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR). Derived from site: In situ; Kidney; UBERON=UBERON_0002113. Cell type: Epithelial cell of kidney; CL=CL_0002518. |
| Species of origin | Canis lupus familiaris (Dog) (Canis familiaris)
(NCBI Taxonomy: 9615)
Breed/subspecies: Cocker Spaniel; VBO=VBO_0200372. |
| Hierarchy | Parent: CVCL_D5J1 (MDCK-WT-EKARrEV-NLS-LDR) |
| Sex of cell | Female |
| Age at sampling | Adult |
| Category | Transformed cell line |
| Publications | PubMed=34667080; DOI=10.26508/lsa.202101206; PMCID=PMC8548211 |
| Cross-references | |
| Encyclopedic resources | Wikidata; Q127382792 |
| Entry history | |
| Entry creation | 02-May-2024 |
| Last entry update | 14-Aug-2025 |
| Version number | 5 |