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Cellosaurus A-431 (CVCL_0037)

[Text version]
Cell line name A-431
Synonyms A431; A431/P
Accession CVCL_0037
Resource Identification Initiative To cite this cell line use: A-431 (RRID:CVCL_0037)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).
Population: Caucasian.
Doubling time: ~80-100 hours (DSMZ=ACC-91).
Karyotypic information: Hypertriploid karyotype (ATCC=CRL-1555).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Cell surface proteome.
Omics: Deep antibody staining analysis.
Omics: Deep exome analysis.
Omics: Deep phosphoproteome analysis.
Omics: Deep proteome analysis.
Omics: Deep quantitative proteome analysis.
Omics: DNA methylation analysis.
Omics: HLA class I peptidome analysis by proteomics.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Derived from site: In situ; Skin, epidermis; UBERON=UBERON_0001003.
Sequence variations
  • Gene fusion; HGNC; 3236; EGFR + HGNC; 9237; PPARGC1A; Name(s)=EGFR-PPARGC1A (PubMed=23637631).
  • Mutation; HGNC; 11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Unspecified (PubMed=1394225).
HLA typing Source: PubMed=25960936
Class I

Source: PubMed=26589293
Class I
Class II
Genome ancestry Source: PubMed=30894373

Origin% genome
Native American0
East Asian, North7.84
East Asian, South0
South Asian6.03
European, North17.15
European, South65.16
Disease Skin squamous cell carcinoma (NCIt: C4819)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_C8WW (A-431-Luc)CVCL_A4BS (A-431-Luc2)CVCL_C8Z1 (A-431-SF)
CVCL_QX11 (A431 siYAP)CVCL_QX12 (A431 YAP-5SA)CVCL_B5YD (A431-AC3)
CVCL_EQ67 (A431-PR)CVCL_GY97 (A431/CDDP1)CVCL_GY98 (A431/CDDP2)
CVCL_4V41 (A431/TPT)CVCL_3485 (A431NS)CVCL_WS72 (A431PF)
CVCL_B1BR (Abcam A-431 ADRB2 KO)CVCL_B1BS (Abcam A-431 B2M KO)CVCL_B1BT (Abcam A-431 BST2 KO)
CVCL_B1BU (Abcam A-431 CAV1 KO)CVCL_B1BV (Abcam A-431 CBFB KO)CVCL_B1BW (Abcam A-431 CENPB KO)
CVCL_B1BX (Abcam A-431 CLDN1 KO)CVCL_B1BY (Abcam A-431 CTNNB1 KO)CVCL_B1BZ (Abcam A-431 CTSD KO)
CVCL_B1C0 (Abcam A-431 DDAH1 KO)CVCL_B1C1 (Abcam A-431 DHCR24 KO)CVCL_B1C2 (Abcam A-431 DPF2 KO)
CVCL_B1C3 (Abcam A-431 DUSP6 KO)CVCL_B1C4 (Abcam A-431 EPCAM KO)CVCL_B1C5 (Abcam A-431 G3BP1 KO)
CVCL_B1C6 (Abcam A-431 GRB2 KO)CVCL_B1C7 (Abcam A-431 H1-0 KO)CVCL_B1C8 (Abcam A-431 HLA-A KO)
CVCL_B1C9 (Abcam A-431 HSP90AB1 KO)CVCL_B1CA (Abcam A-431 IL1RN KO)CVCL_B1CB (Abcam A-431 KRT13 KO)
CVCL_B1CC (Abcam A-431 KRT14 KO)CVCL_B1CD (Abcam A-431 KRT15 KO)CVCL_B1CE (Abcam A-431 MMP14 KO)
CVCL_B1CF (Abcam A-431 MYL9 KO)CVCL_B1CG (Abcam A-431 NBN KO)CVCL_B1CS (Abcam A-431 NDRG1 KO)
CVCL_B1CH (Abcam A-431 NEK9 KO)CVCL_B1CI (Abcam A-431 NT5E KO)CVCL_B1CJ (Abcam A-431 P4HB KO)
CVCL_B1CK (Abcam A-431 PERP KO)CVCL_B1CL (Abcam A-431 PPP2R5D KO)CVCL_B1CM (Abcam A-431 PXN KO)
CVCL_B1CN (Abcam A-431 SOS1 KO)CVCL_B1CP (Abcam A-431 STK24 KO)CVCL_B1CQ (Abcam A-431 VAMP8 KO)
CVCL_B1CR (Abcam A-431 VCL KO)
Sex of cell Female
Age at sampling 85Y
Category Cancer cell line
STR profile Source(s): AddexBio=C0012001/22; ATCC=CRL-1555; CCRID; CLS=300112; Cosmic-CLP=910925; DepMap=ACH-001328; DSMZ=ACC-91; Technion Genomics Center BCF; IZSLER=BS TCL 161; JCRB=IFO50411; JCRB=JCRB0004; KCLB=21555; KCLB=80005; PubMed=11416159; PubMed=25877200; RCB=RCB0202; RCB=RCB1872; TKG=TKG 0182

AmelogeninX (ATCC=CRL-1555; CCRID; CLS=300112; Cosmic-CLP=910925; DepMap=ACH-001328; DSMZ=ACC-91; Technion Genomics Center BCF; IZSLER=BS TCL 161; JCRB=IFO50411; JCRB=JCRB0004; KCLB=21555; PubMed=11416159; PubMed=25877200; RCB=RCB0202; RCB=RCB1872; TKG=TKG 0182)
X,Y (AddexBio=C0012001/22)
D3S135814 (ATCC=CRL-1555; CLS=300112; DepMap=ACH-001328; DSMZ=ACC-91; KCLB=21555; KCLB=80005; Technion Genomics Center BCF; PubMed=25877200)
14,20 (CCRID)
D5S81811,13 (AddexBio=C0012001/22)
12,13 (ATCC=CRL-1555; CCRID; CLS=300112; Cosmic-CLP=910925; DepMap=ACH-001328; DSMZ=ACC-91; Technion Genomics Center BCF; IZSLER=BS TCL 161; JCRB=IFO50411; JCRB=JCRB0004; KCLB=21555; KCLB=80005; PubMed=25877200; RCB=RCB0202; RCB=RCB1872; TKG=TKG 0182)
D19S43315,15.2 (ATCC=CRL-1555; CCRID; Technion Genomics Center BCF)
15,16 (DSMZ=ACC-91)
D21S1128 (ATCC=CRL-1555; CCRID; DepMap=ACH-001328; Technion Genomics Center BCF; IZSLER=BS TCL 161; PubMed=25877200)
28,30 (CLS=300112; DSMZ=ACC-91; PubMed=11416159)
Penta D9,11 (CLS=300112; DSMZ=ACC-91)
11 (ATCC=CRL-1555; DepMap=ACH-001328; Technion Genomics Center BCF; PubMed=25877200)
Penta E12,13

Run an STR similarity search on this cell line
Web pages https://en.wikipedia.org/wiki/A431_cells

PubMed=4357758; DOI=10.1093/jnci/51.5.1417
Giard D.J., Aaronson S.A., Todaro G.J., Arnstein P., Kersey J.H., Dosik H., Parks W.P.
In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors.
J. Natl. Cancer Inst. 51:1417-1423(1973)

Todaro G.J., De Larco J.E.
Growth factors produced by sarcoma virus-transformed cells.
Cancer Res. 38:4147-4154(1978)

PubMed=6254071; DOI=10.1073/pnas.77.9.5258
Todaro G.J., Fryling C.M., De Larco J.E.
Transforming growth factors produced by certain human tumor cells: polypeptides that interact with epidermal growth factor receptors.
Proc. Natl. Acad. Sci. U.S.A. 77:5258-5262(1980)

PubMed=22282976; DOI=10.1093/carcin/1.1.21
Day R.S. III, Ziolkowski C.H.J., Scudiero D.A., Meyer S.A., Mattern M.R.
Human tumor cell strains defective in the repair of alkylation damage.
Carcinogenesis 1:21-32(1980)

PubMed=6954533; DOI=10.1073/pnas.79.7.2194
Westin E.H., Gallo R.C., Arya S.K., Eva A., Souza L.M., Baluda M.A., Aaronson S.A., Wong-Staal F.
Differential expression of the amv gene in human hematopoietic cells.
Proc. Natl. Acad. Sci. U.S.A. 79:2194-2198(1982)

PubMed=6983291; DOI=10.1002/aja.1001650207
Barka T., van der Noen H.M.
Culture of A-431 human epidermoid carcinoma cells in serum-free medium: effect of culture conditions on the binding of [125I]-epidermal growth factor.
Am. J. Anat. 165:187-198(1982)

PubMed=6092175; DOI=10.1016/0303-7207(84)90053-4
Gamou S., Kim Y.S., Shimizu N.
Different responses to EGF in two human carcinoma cell lines, A431 and UCVA-1, possessing high numbers of EGF receptors.
Mol. Cell. Endocrinol. 37:205-213(1984)

PubMed=6500159; DOI=10.1159/000163283
Gershwin M.E., Lentz D., Owens R.B.
Relationship between karyotype of tissue culture lines and tumorigenicity in nude mice.
Exp. Cell Biol. 52:361-370(1984)

Yee C., Krishnan-Hewlett I., Baker C.C., Schlegel R., Howley P.M.
Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines.
Am. J. Pathol. 119:361-366(1985)

Pekkola-Heino K., Kulmala J., Grenman S.E., Carey T.E., Grenman R.
Radiation response of vulvar squamous cell carcinoma (UM-SCV-1A, UM-SCV-1B, UM-SCV-2, and A-431) cells in vitro.
Cancer Res. 49:4876-4878(1989)

PubMed=2228902; DOI=10.1007/BF02624609
Rikimaru K., Toda H., Tachikawa N., Kamata N., Enomoto S.
Growth of the malignant and nonmalignant human squamous cells in a protein-free defined medium.
In Vitro Cell. Dev. Biol. 26:849-856(1990)

Somers K.D., Merrick M.A., Lopez M.E., Incognito L.S., Schechter G.L., Casey G.
Frequent p53 mutations in head and neck cancer.
Cancer Res. 52:5997-6000(1992)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

PubMed=9887230; DOI=10.1006/gyno.1998.5194
Rantanen V., Grenman S.E., Kurvinen K., Hietanen S., Raitanen M., Syrjanen S.M.
p53 mutations and presence of HPV DNA do not correlate with radiosensitivity of gynecological cancer cell lines.
Gynecol. Oncol. 71:352-358(1998)

PubMed=11416159; DOI=10.1073/pnas.121616198
Masters J.R.W., Thomson J.A., Daly-Burns B., Reid Y.A., Dirks W.G., Packer P., Toji L.H., Ohno T., Tanabe H., Arlett C.F., Kelland L.R., Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.
Short tandem repeat profiling provides an international reference standard for human cell lines.
Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)

PubMed=11668190; DOI=10.1177/002215540104901105
Quentmeier H., Osborn M., Reinhardt J., Zaborski M., Drexler H.G.
Immunocytochemical analysis of cell lines derived from solid tumors.
J. Histochem. Cytochem. 49:1369-1378(2001)

PubMed=17178626; DOI=10.1080/09553000600969812
Kurvinen K., Rantanen V., Syrjanen S.M., Johansson B.
Radiation-induced effects on telomerase in gynecological cancer cell lines with different radiosensitivity and repair capacity.
Int. J. Radiat. Biol. 82:859-867(2006)

PubMed=20164919; DOI=10.1038/nature08768
Bignell G.R., Greenman C.D., Davies H., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=22108792; DOI=10.1038/msb.2011.84
Munoz J., Low T.Y., Kok Y.J., Chin A., Frese C.K., Ding V., Choo A.B.-H., Heck A.J.R.
The quantitative proteomes of human-induced pluripotent stem cells and embryonic stem cells.
Mol. Syst. Biol. 7:550-550(2011)

PubMed=23637631; DOI=10.1371/journal.pgen.1003464
Giacomini C.P., Sun S., Varma S., Shain A.H., Giacomini M.M., Balagtas J.M.S., Sweeney R.T., Lai E., Del Vecchio C.A., Forster A.D., Clarke N., Montgomery K.D., Zhu S., Wong A.J., van de Rijn M., West R.B., Pollack J.R.
Breakpoint analysis of transcriptional and genomic profiles uncovers novel gene fusions spanning multiple human cancer types.
PLoS Genet. 9:E1003464-E1003464(2013)

PubMed=24804581; DOI=10.1021/cb500116c
Giansanti P., Preisinger C., Huber K.V.M., Gridling M., Superti-Furga G., Bennett K.L., Heck A.J.R.
Evaluating the promiscuous nature of tyrosine kinase inhibitors assessed in A431 epidermoid carcinoma cells by both chemical- and phosphoproteomics.
ACS Chem. Biol. 9:1490-1498(2014)

PubMed=25960936; DOI=10.4161/21624011.2014.954893
Boegel S., Lower M., Bukur T., Sahin U., Castle J.C.
A catalog of HLA type, HLA expression, and neo-epitope candidates in human cancer cell lines.
OncoImmunology 3:e954893.1-e954893.12(2014)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25877200; DOI=10.1038/nature14397
Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=25894527; DOI=10.1371/journal.pone.0121314
Bausch-Fluck D., Hofmann A., Bock T., Frei A.P., Cerciello F., Jacobs A., Moest H., Omasits U., Gundry R.L., Yoon C., Schiess R., Schmidt A., Mirkowska P., Hartlova A.S., Van Eyk J.E., Bourquin J.-P., Aebersold R., Boheler K.R., Zandstra P.W., Wollscheid B.
A mass spectrometric-derived cell surface protein atlas.
PLoS ONE 10:E0121314-E0121314(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5
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Genome Med. 7:118.1-118.7(2015)

PubMed=26989177; DOI=10.1242/dev.133728
Elbediwy A., Vincent-Mistiaen Z.I., Spencer-Dene B., Stone R.K., Boeing S., Wculek S.K., Cordero J., Tan E.H., Ridgway R., Brunton V.G., Sahai E., Gerhardt H., Behrens A., Malanchi I., Sansom O.J., Thompson B.J.
Integrin signalling regulates YAP and TAZ to control skin homeostasis.
Development 143:1674-1687(2016)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=28176443; DOI=10.1002/pmic.201600254
Tiwari R., Sahu I., Soni B.L., Sathe G.J., Datta K.K., Thapa P., Sinha S., Vadivel C.K., Dhaka B., Gowda H., Vaidya M.M.
Quantitative phosphoproteomic analysis reveals system-wide signaling pathways regulated by site-specific phosphorylation of keratin-8 in skin squamous cell carcinoma derived cell line.
Proteomics 17:1600254.1-1600254.17(2017)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cell line collections (Providers) Abcam; ab263975
Abcam; ab275462
AddexBio; C0012001/22
ATCC; CRL-1555
ATCC; CRL-7907 - Discontinued
BCRC; 60161
BCRJ; 0032
CLS; 300112
ECACC; 85090402
JCRB; IFO50411
KCB; KCB 200651YJ
KCLB; 21555
KCLB; 80005
NCBI_Iran; C204
RCB; RCB0202
RCB; RCB1872
TKG; TKG 0182
Ubigene; YC-D016
Cell line databases/resources CLO; CLO_0001591
CLO; CLO_0001592
CLO; CLO_0050011
CLO; CLO_0050012
MCCL; MCC:0000025
CLDB; cl155
CLDB; cl156
CLDB; cl157
CLDB; cl159
CLDB; cl160
CLDB; cl161
cancercelllines; CVCL_0037
CCRID; 1101HUM-PUMC000092
Cell_Model_Passport; SIDM00995
Cosmic-CLP; 910925
DepMap; ACH-001328
DSMZCellDive; ACC-91
Lonza; 814
TOKU-E; 476
Anatomy/cell type resources BTO; BTO:0000017
Biological sample resources BioSample; SAMN01821614
BioSample; SAMN01821671
BioSample; SAMN01821724
BioSample; SAMN03472220
CRISP screens repositories BioGRID_ORCS_Cell_line; 1572
Chemistry resources ChEMBL-Cells; CHEMBL3307523
ChEMBL-Targets; CHEMBL614069
GDSC; 910925
PharmacoDB; A431_46_2019
PubChem_Cell_line; CVCL_0037
Encyclopedic resources Wikidata; Q4649310
Experimental variables resources EFO; EFO_0006268
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-2706
ArrayExpress; E-MTAB-3610
GEO; GSM183432
GEO; GSM183433
GEO; GSM827302
GEO; GSM1374384
GEO; GSM1669586
GEO; GSM2112551
GEO; GSM2112555
Polymorphism and mutation databases Cosmic; 688038
Cosmic; 735609
Cosmic; 755305
Cosmic; 876559
Cosmic; 897743
Cosmic; 910564
Cosmic; 910925
Cosmic; 974317
Cosmic; 1071898
Cosmic; 1176624
Cosmic; 1351516
Cosmic; 1477434
Cosmic; 1945879
Cosmic; 1995334
Cosmic; 2131571
Cosmic; 2301592
Cosmic; 2646654
IARC_TP53; 21167
IARC_TP53; 23618
Progenetix; CVCL_0037
Proteomic databases PRIDE; PXD000065
PRIDE; PXD000589
PRIDE; PXD000681
PRIDE; PXD001582
PRIDE; PXD003937
PRIDE; PXD006291
PRIDE; PXD005301
PRIDE; PXD007206
PRIDE; PXD008996
PRIDE; PXD017536
PRIDE; PXD030304
PRIDE; PXD030984
PRIDE; PXD033685
Sequence databases EGA; EGAS00001000610
EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update02-May-2024
Version number48