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Cellosaurus 143B (CVCL_2270)

[Text version]
Cell line name 143B
Synonyms 143b; 143 B; 143B TK-; 143B.TK-; 143BTK-; 143TK-; HOS-143B; HOS-143b; GM05887; GM05887A
Accession CVCL_2270
Resource Identification Initiative To cite this cell line use: 143B (RRID:CVCL_2270)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-8303.
Population: Caucasian.
Doubling time: ~36 hours (PubMed=21519327).
Omics: Genomics; ChIP-seq; H3K27ac.
Omics: Genomics; ChIP-seq; H3K4me1.
Omics: Genomics; Whole exome sequencing.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Caution: Could be identical to HTK- (Cellosaurus=CVCL_2522).
Misspelling: I43B; Cosmic=1529910.
Derived from site: In situ; Bone, right femur; UBERON=UBERON_0000981.
Sequence variations
  • Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (PubMed=19787792).
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Heterozygous (DepMap=ACH-001001).
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Ala59Thr (c.175G>A); ClinVar=VCV000012581; Zygosity=Heterozygous (DepMap=ACH-001001).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg156Pro (c.467G>C); ClinVar=VCV000634768; Zygosity=Homozygous (PubMed=19787792; DepMap=ACH-001001).
HLA typing Source: PubMed=26589293
Class I
HLA-AA*02:11,02:11
HLA-BB*52:01,52:01
HLA-CC*12:02,12:02
Class II
HLA-DQDQB1*05:02,05:02
HLA-DRDRB1*15:02,16:05
Genome ancestry Source: PubMed=30894373

Origin% genome
African0
Native American0
East Asian, North0
East Asian, South4.02
South Asian37
European, North0
European, South58.98
Disease Osteosarcoma (NCIt: C9145)
Osteosarcoma (ORDO: Orphanet_668)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0312 (HOS)
Children:
CVCL_3476 (143.98.2)CVCL_3477 (143B pML BK TK)CVCL_ZL52 (143B rho-0 EtBr)
CVCL_XF76 (143B rho-0#25)CVCL_C0Q7 (143B-GFP-HK2)CVCL_U923 (143B.206)
CVCL_9W36 (143BTK-)CVCL_IW03 (GM16530)CVCL_C6V0 (HOS-143B/CISR8)
CVCL_C6V1 (HOS-143B/DOXR8)CVCL_C6V2 (HOS-143B/MTXR8)CVCL_C6V3 (HOS-143B/TRIR8)
Sex of cell Female
Age at sampling 13Y
Category Cancer cell line
STR profile Source(s): ATCC=CRL-8303; BCRC=60439; CCRID; CLS=305232; ECACC=91112502; IZSLER=BS TCL 77; PubMed=19787792; PubMed=25877200

Markers:
AmelogeninX
CSF1PO12
D2S133824,25
D3S135815
D5S81813 (ATCC=CRL-8303; BCRC=60439; CCRID; CLS=305232; ECACC=91112502; IZSLER=BS TCL 77; PubMed=25877200)
13,14 (PubMed=19787792)
D7S82011,12
D8S117911,14
D13S31712
D16S53910,13
D18S5117
D19S43313
D21S1131.2,32.2
FGA24
Penta D9,10
Penta E7,12
SE3321
TH016
TPOX11
vWA18 (ATCC=CRL-8303; BCRC=60439; CCRID; CLS=305232; ECACC=91112502; IZSLER=BS TCL 77; PubMed=25877200)
18,19 (PubMed=19787792)

Run an STR similarity search on this cell line
Web pages Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/1/cell-lines-detail-310.html
Publications

PubMed=2233717; DOI=10.1128/mcb.10.11.5772-5781.1990; PMCID=PMC361354
Lisa Diller, Jayne Kassel, Camille E. Nelson, Magdalena A. Gryka, Gregory Litwak, Mark Gebhardt, Brigitte Bressac, Mehmet Ozturk, Suzanne J. Baker, Bert Vogelstein, Stephen H. Friend;
p53 functions as a cell cycle control protein in osteosarcomas.
Mol. Cell. Biol. 10:5772-5781(1990)

CLPUB00447
Richard A. Mulivor, Sharon F. Suchy;
1992/1993 catalog of cell lines. NIGMS human genetic mutant cell repository. 16th edition. October 1992.
(In misc. document) Institute for Medical Research (Camden, N.J.) NIH 92-2011; pp.1-918; National Institutes of Health; Bethesda; USA (1992)

PubMed=19787792; DOI=10.1002/gcc.20717
Laura Ottaviano, Karl-Ludwig Schaefer, Melanie Gajewski, Wolfgang Huckenbeck, Stefan E. Baldus, Uwe Rogel, Carlos Mackintosh, Enrique de Alava, Ola Myklebost, Stine Henrichson Kresse, Leonardo Andres Meza-Zepeda ...Show all 18 authors... , Massimo Serra, Anne-Marie Cleton-Jansen, Pancras Cornelis Wilhelmus Hogendoorn, Horst Buerger, Thomas Aigner, Helmut Erich Gabbert, Christopher Poremba; Show fewer authors
Molecular characterization of commonly used cell lines for bone tumor research: a trans-European EuroBoNet effort.
Genes Chromosomes Cancer 49:40-51(2010)

PubMed=21519327; DOI=10.1038/labinvest.2011.72
Alexander B. Mohseny, Isidro Machado, Yong-Ping Cai, Karl-Ludwig Schaefer, Massimo Serra, Pancras Cornelis Wilhelmus Hogendoorn, Antonio Llombart-Bosch, Anne-Marie Cleton-Jansen;
Functional characterization of osteosarcoma cell lines provides representative models to study the human disease.
Lab. Invest. 91:1195-1205(2011)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Jordi Ginesta Barretina, Giordano Caponigro, Nicolas Stransky, Kavitha Venkatesan, Adam A. Margolin, Sungjoon Kim, Christopher J. Wilson, Joseph Lehar, Gregory V. Kryukov, Dmitriy Sonkin, Anupama Reddy ...Show all 61 authors... , Manway Liu, Lauren Murray, Michael F. Berger, John E. Monahan, Paula Morais, Jodi Meltzer, Adam Korejwa, Judit Jane-Valbuena, Felipa A. Mapa, Joseph Thibault, Eva Bric-Furlong, Pichai Raman, Aaron Shipway, Ingo H. Engels, Jill Cheng, Guo-Ying K. Yu, Jian-Jun Yu, Peter Aspesi Jr., Melanie de Silva, Kalpana Jagtap, Michael D. Jones, Li Wang, Charles Hatton, Emanuele Palescandolo, Supriya Gupta, Scott Mahan, Carrie Sougnez, Robert C. Onofrio, Ted Liefeld, Laura E. MacConaill, Wendy Winckler, Michael Reich, Nan-Xin Li, Jill P. Mesirov, Stacey B. Gabriel, Gad Getz, Kristin Ardlie, Vivien Chan, Vic E. Myer, Barbara L. Weber, Jeff Porter, Markus Warmuth, Peter Finan, Jennifer L. Harris, Matthew Langer Meyerson, Todd Robert Golub, Michael P. Morrissey, William Raj Sellers, Robert Schlegel, Levi Alexander Garraway; Show fewer authors
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=23144859; DOI=10.1371/journal.pone.0048262; PMCID=PMC3492335
Stine Henrichson Kresse, Halfdan Rydbeck, Magne Skarn, Heidi Maria Namlos, Ana Heslanyeth Barragan-Polania, Anne-Marie Cleton-Jansen, Massimo Serra, Knut Liestol, Pancras Cornelis Wilhelmus Hogendoorn, Eivind Hovig, Ola Myklebost, Leonardo Andres Meza-Zepeda;
Integrative analysis reveals relationships of genetic and epigenetic alterations in osteosarcoma.
PLoS ONE 7:e48262.1-e48262.20(2012)

PubMed=25485619; DOI=10.1038/nbt.3080
Christiaan Klijn, Steffen Durinck, Eric W. Stawiski, Peter M. Haverty, Zhao-Shi Jiang, Han-Bin Liu, Jeremiah Degenhardt, Oleg Mayba, Florian Gnad, Jin-Feng Liu, Gregoire Pau ...Show all 30 authors... , Jens Reeder, Yi Cao, Kiran Mukhyala, Suresh K. Selvaraj, Ma-Mie Yu, Gregory J. Zynda, Matthew J. Brauer, Thomas D. Wu, Robert Clifford Gentleman, Gerard Manning, Robert L. Yauch, Richard Bourgon, David Stokoe, Zora Modrusan, Richard M. Neve, Frederic J. de Sauvage, Jeffrey Settleman, Somasekar Seshagiri, Ze-Min Zhang; Show fewer authors
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25877200; DOI=10.1038/nature14397
Ma-Mie Yu, Suresh K. Selvaraj, May M.Y. Liang-Chu, Sahar Aghajani, Matthew Busse, Jean Yuan, Genee Lee, Franklin V. Peale, Christiaan Klijn, Richard Bourgon, Joshua S. Kaminker, Richard M. Neve;
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=26320182; DOI=10.18632/oncotarget.5177; PMCID=PMC4745740
Ling Ren, Arnulfo Mendoza, Jack Zhu, Joseph W. Briggs, Charles Halsey, Ellen S. Hong, Sandra Sczerba Burkett, James J. Morrow, Michael M. Lizardo, Tanasa Osborne, Samuel Q. Li ...Show all 14 authors... , Hue H. Luu, Paul S. Meltzer, Chand Khanna; Show fewer authors
Characterization of the metastatic phenotype of a panel of established osteosarcoma cells.
Oncotarget 6:29469-29481(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Jelle Scholtalbers, Sebastian Boegel, Thomas Bukur, Marius Byl, Sebastian Goerges, Patrick Sorn, Martin Loewer, Ugur Sahin, John C. Castle;
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=26852163; DOI=10.1074/mcp.M115.053181; PMCID=PMC4858937
Ina Aretz, Christopher Hardt, Ilka Wittig, David Meierhofer;
An impaired respiratory electron chain triggers down-regulation of the energy metabolism and de-ubiquitination of solute carrier amino acid transporters.
Mol. Cell. Proteomics 15:1526-1538(2016)

PubMed=29334376; DOI=10.1038/nm.4475; PMCID=PMC5803371
James J. Morrow, Ian Bayles, Alister Peter William Funnell, Tyler Eugene Miller, Alina Saiakhova, Michael M. Lizardo, Cynthia F. Bartels, Maaike Y. Kapteijn, Stevephen Hung, Arnulfo Mendoza, Gursimran Dhillon ...Show all 26 authors... , Daniel R. Chee, Jay T. Myers, Frederick Allen, Marco Gambarotti, Alberto Righi, Analisa DiFeo, Brian P. Rubin, Alex Y. Huang, Paul S. Meltzer, Lee J. Helman, Piero Picci, Hendrik Henri Versteeg, John A. Stamatoyannopoulos, Chand Khanna, Peter C. Scacheri; Show fewer authors
Positively selected enhancer elements endow osteosarcoma cells with metastatic competence.
Nat. Med. 24:176-185(2018)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=36480329; DOI=10.1002/adbi.202200194
Kaan Low, Frank Hills, Helen C. Roberts, Britta Stordal;
Establishment and characterization of single and triple-agent resistant osteosarcoma cell lines.
Adv. Biol. (Weinh.) 7:e2200194.1-e2200194.13(2023)

Cross-references
Cell line collections (Providers) ATCC; CRL-8303
BCRC; 60439
CCTCC; GDC0076
CLS; 305232
Coriell; GM05887
ECACC; 91112502
IZSLER; BS TCL 77
Cell line databases/resources CLO; CLO_0001084
CLO; CLO_0001088
CLO; CLO_0024221
CLDB; cl10
CLDB; cl11
cancercelllines; CVCL_2270
CCRID; 1101HUM-PUMC000363
CCRID; 4201HUM-CCTCC00076
Cell_Model_Passport; SIDM00804
DepMap; ACH-001001
LINCS_LDP; LCL-1417
Lonza; 136
TOKU-E; 132
Anatomy/cell type resources BTO; BTO_0003167
Biological sample resources BioSample; SAMN03471904
CRISP screens repositories BioGRID_ORCS_Cell_line; 315
Chemistry resources ChEMBL-Cells; CHEMBL3307382
ChEMBL-Targets; CHEMBL614508
PharmacoDB; 143B_3_2019
PubChem_Cell_line; CVCL_2270
Encyclopedic resources Wikidata; Q54581813
Experimental variables resources EFO; EFO_0006355
Gene expression databases ArrayExpress; E-MTAB-2706
GEO; GSM320829
GEO; GSM879206
GEO; GSM886836
GEO; GSM887899
GEO; GSM1914980
GEO; GSM1914981
GEO; GSM1914982
GEO; GSM1914983
GEO; GSM1915018
GEO; GSM1915019
GEO; GSM1915020
GEO; GSM1915021
GEO; GSM1915022
GEO; GSM2635309
GEO; GSM2635310
Polymorphism and mutation databases Cosmic; 1074398
Cosmic; 1529910
Cosmic; 2009541
IARC_TP53; 21149
IARC_TP53; 27023
Progenetix; CVCL_2270
Proteomic databases PRIDE; PXD002425
Sequence databases EGA; EGAS00001000610
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number43