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Cellosaurus 143B rho-0#25 (CVCL_XF76)

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Cell line name 143B rho-0#25
Accession CVCL_XF76
Resource Identification Initiative To cite this cell line use: 143B rho-0#25 (RRID:CVCL_XF76)
Comments Population: Caucasian.
Characteristics: Cell line devoid of mitochondrial DNA (rho0). Produced by transient transfection with plasmid pMA4008 encoding HSV-1 UL12.5M185 protein, which damages mtDNA by an unknown mechanism (PubMed=27136098).
Derived from site: In situ; Bone, right femur; UBERON=UBERON_0000981.
Sequence variations
  • Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Ala59Thr (c.175G>A); ClinVar=VCV000012581; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg156Pro (c.467G>C); ClinVar=VCV000634768; Zygosity=Homozygous (from parent cell line).
Disease Osteosarcoma (NCIt: C9145)
Osteosarcoma (ORDO: Orphanet_668)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_2270 (143B)
Sex of cell Female
Age at sampling 13Y
Category Cancer cell line
Publications

PubMed=27136098; DOI=10.1371/journal.pone.0154684; PMCID=PMC4852919
Domenico Spadafora, Nataliya Kozhukhar, Vladimir N. Chouljenko, Konstantin Gus Kousoulas, Mikhail F. Alexeyev;
Methods for efficient elimination of mitochondrial DNA from cultured cells.
PLoS ONE 11:e0154684.1-e0154684.18(2016)

Cross-references
Cell line collections (Providers) Kerafast; ESA106
Cell line databases/resources cancercelllines; CVCL_XF76
Encyclopedic resources Wikidata; Q93299114
Entry history
Entry creation06-Sep-2019
Last entry update19-Dec-2024
Version number12