ID   143B rho-0#25
AC   CVCL_XF76
DR   cancercelllines; CVCL_XF76
DR   Kerafast; ESA106
DR   Wikidata; Q93299114
RX   PubMed=27136098;
CC   Population: Caucasian.
CC   Characteristics: Cell line devoid of mitochondrial DNA (rho0). Produced by transient transfection with plasmid pMA4008 encoding HSV-1 UL12.5M185 protein, which damages mtDNA by an unknown mechanism (PubMed=27136098).
CC   Sequence variation: Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Ala59Thr (c.175G>A); ClinVar=VCV000012581; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg156Pro (c.467G>C); ClinVar=VCV000634768; Zygosity=Homozygous (from parent cell line).
CC   Derived from site: In situ; Bone, right femur; UBERON=UBERON_0000981.
DI   NCIt; C9145; Osteosarcoma
DI   ORDO; Orphanet_668; Osteosarcoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_2270 ! 143B
SX   Female
AG   13Y
CA   Cancer cell line
DT   Created: 06-09-19; Last updated: 19-12-24; Version: 12
//
RX   PubMed=27136098; DOI=10.1371/journal.pone.0154684; PMCID=PMC4852919;
RA   Spadafora, Domenico
RA   Kozhukhar, Nataliya
RA   Chouljenko, Vladimir N.
RA   Kousoulas, Konstantin Gus
RA   Alexeyev, Mikhail F.
RT   "Methods for efficient elimination of mitochondrial DNA from cultured
RT   cells.";
RL   PLoS ONE 11:e0154684.1-e0154684.18(2016).
//