Cellosaurus cell line HyCyte 143B KO-hIBSP (CVCL

Cross-references
Cell line name	HyCyte 143B KO-hIBSP
Synonyms	hIBSP knockout 143B
Accession	CVCL_F1LH
Resource Identification Initiative	To cite this cell line use: HyCyte 143B KO-hIBSP (RRID:CVCL_F1LH)
Comments	Population: Caucasian. Doubling time: 36-48 hours (Hysigen=CGKO-M3273). Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:5341; IBSP. Derived from site: In situ; Bone, right femur; UBERON=UBERON_0000981.
Sequence variations	Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line). Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Ala59Thr (c.175G>A); ClinVar=VCV000012581; Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg156Pro (c.467G>C); ClinVar=VCV000634768; Zygosity=Homozygous (from parent cell line).
Disease	Osteosarcoma (NCIt: C9145) Osteosarcoma (ORDO: Orphanet_668)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_2270 (143B)
Sex of cell	Female
Age at sampling	13Y
Category	Cancer cell line
Cell line collections (Providers)	Hysigen; CGKO-M3273
Entry history
Entry creation	27-Nov-2025
Last entry update	27-Nov-2025
Version number	1