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Cellosaurus PC12 (CVCL_0481)

[Text version]
Cell line name PC12
Synonyms PC-12; PC 12; PC12.1
Accession CVCL_0481
Resource Identification Initiative To cite this cell line use: PC12 (RRID:CVCL_0481)
Comments Group: Space-flown cell line (cellonaut).
Characteristics: Responds reversibly to NGF by induction of a neuronal phenotype.
Doubling time: 40 hours (PubMed=9886495); ~50-60 hours (DSMZ=ACC-159); 92 hours (CLS; ECACC=88022401).
Derived from sampling site: Adrenal gland.
Disease Rat adrenal gland pheochromocytoma (NCIt: C121568)
Species of origin Rattus norvegicus (Rat) (NCBI Taxonomy: 10116)
Breed/subspecies: NEDH.
Hierarchy Children:
CVCL_0109 (123.7)CVCL_0133 (A126-1B2)CVCL_X983 (A208-4)
CVCL_X984 (A208-7)CVCL_X985 (AB.11)CVCL_YJ89 (LINTERNA PC-12)
CVCL_JY59 (Neuroscreen-1)CVCL_F659 (PC-12 Adh)CVCL_AU97 (PC-12 HD-Q23 cell 14)
CVCL_AU98 (PC-12 HD-Q23 cell 7)CVCL_AU99 (PC-12 HD-Q74 cell 10)CVCL_AV00 (PC-12 HD-Q74 cell 1b)
CVCL_5574 (PC-12TG)CVCL_C0PY (PC12 6c)CVCL_DN60 (PC12 HTT-SW2-Q145 clone 14)
CVCL_DN61 (PC12 HTT-SW2-Q145-TagRFP clone 5)CVCL_DN62 (PC12 HTT-SW2-Q23 clone 28)CVCL_DN63 (PC12 HTT-SW2-Q23-TagRFP clone 34)
CVCL_DN64 (PC12 HTT-SW2-Q73 clone 29)CVCL_DN65 (PC12 HTT-SW2-Q73-TagRFP clone 17)CVCL_V361 (PC12 Tet-Off)
CVCL_V333 (PC12 Tet-On)CVCL_C0PQ (PC12-1)CVCL_C0PR (PC12-15)
CVCL_C0PS (PC12-16A)CVCL_C0PT (PC12-19)CVCL_VH13 (PC12-1G2)
CVCL_C0PU (PC12-21)CVCL_C0PV (PC12-27)CVCL_VH10 (PC12-2B3)
CVCL_C130 (PC12-615)CVCL_J442 (PC12-AC)CVCL_VH11 (PC12-C3)
CVCL_VH12 (PC12-D60)CVCL_T752 (PC12-F7)CVCL_C0PX (PC12-FR)
CVCL_T753 (PC12-G11)CVCL_C4US (PC12-P1D10)CVCL_C4UT (PC12-P1F1)
CVCL_C0PP (PC12.4)CVCL_5573 (PC12.P3)CVCL_4681 (PC12D)
CVCL_JX88 (PC12h)CVCL_J438 (PC12HS)CVCL_J440 (PC12IS)
CVCL_4925 (PC12K)CVCL_J441 (PC12MS)CVCL_C126 (PC12nnr14)
CVCL_C127 (PC12nnr3)CVCL_C128 (PC12nnr5)CVCL_C129 (PC12nnr6)
CVCL_0483 (PC2)CVCL_7090 (PC4)CVCL_7091 (PC6)
Sex of cell Male
Age at sampling Age unspecified
Category Cancer cell line
Web pages https://en.wikipedia.org/wiki/PC12_cell
https://www.atcc.org/en/support/technical-support/faqs/pc-12-cells-atcc-crl-1721-vs-atcc-crl-1721-1
http://www.cells-talk.com/index.php/page/copelibrary?key=PC12
https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/p/cell-lines-detail-11.html
Publications

PubMed=1065897; DOI=10.1073/pnas.73.7.2424
Greene L.A., Tischler A.S.
Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor.
Proc. Natl. Acad. Sci. U.S.A. 73:2424-2428(1976)

PubMed=682602
Tischler A.S., Greene L.A.
Morphologic and cytochemical properties of a clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor.
Lab. Invest. 39:77-89(1978)

PubMed=6500171; DOI=10.1016/0303-7207(84)90102-3
Bethea C.L., Kozak S.L.
Effect of extracellular matrix on PC 12 cell shape and dopamine processing.
Mol. Cell. Endocrinol. 37:319-329(1984)

PubMed=2493070; DOI=10.1111/j.1471-4159.1989.tb02538.x
Katoh-Semba R., Oohira A., Sano M., Watanabe K., Kitajima S., Kashiwamata S.
Glycosaminoglycan composition of PC12 pheochromocytoma cells: a comparison with PC12D cells, a new subline of PC12 cells.
J. Neurochem. 52:889-895(1989)

PubMed=2759161; DOI=10.1016/0277-5379(89)90388-X
Chelmicka-Schorr E., Checinski M.E., Arnason B.G.W.
PC12 pheochromocytoma and sympathetic nervous system derived trophic factors augment growth of neuroblastoma.
Eur. J. Cancer Clin. Oncol. 25:1057-1059(1989)

PubMed=2187480; DOI=10.1089/dna.1990.9.221
Muller S.R., Sullivan P.D., Clegg D.O., Feinstein S.C.
Efficient transfection and expression of heterologous genes in PC12 cells.
DNA Cell Biol. 9:221-229(1990)

PubMed=9886495; DOI=10.1002/(SICI)1097-4652(199901)178:1<93::AID-JCP12>3.0.CO;2-U
Dwyer D.S., Liu Y., Bradley R.J.
An ethanol-sensitive variant of the PC12 neuronal cell line: sensitivity to alcohol is associated with increased cell adhesion and decreased glucose accumulation.
J. Cell. Physiol. 178:93-101(1999)

PubMed=15541571; DOI=10.1016/j.mce.2003.02.001
Eaton M.J., Duplan H.
Useful cell lines derived from the adrenal medulla.
Mol. Cell. Endocrinol. 228:39-52(2004)

PubMed=16223334; DOI=10.1290/0411077.1
Dixon D.N., Loxley R.A., Barron A., Cleary S., Phillips J.K.
Comparative studies of PC12 and mouse pheochromocytoma-derived rodent cell lines as models for the study of neuroendocrine systems.
In Vitro Cell. Dev. Biol. Anim. 41:197-206(2005)

PubMed=18005394; DOI=10.1111/j.1748-1716.2007.01805.x
Westerink R.H.S., Ewing A.G.
The PC12 cell as model for neurosecretion.
Acta Physiol. 192:273-285(2008)

PubMed=22538498; DOI=10.1159/000338793
Grozinsky-Glasberg S., Shimon I., Rubinfeld H.
The role of cell lines in the study of neuroendocrine tumors.
Neuroendocrinology 96:173-187(2012)

PubMed=33171774; DOI=10.3390/ijms21218356
Kudo T.-A., Tominami K., Izumi S., Hayashi Y., Noguchi T., Matsuzawa A., Hong G., Nakai J.
Characterization of PC12 cell subclones with different sensitivities to programmed thermal stimulation.
Int. J. Mol. Sci. 21:8356.1-8356.15(2020)

PubMed=34348336; DOI=10.1159/000518337
Delage C., Breard-Mellin L., Theresine C., Simioneck S., Lefranc B., Leprince J., Benard M., Vaudry D.
The heterogeneity of response of PC12 cells from different laboratories to nerve growth factor and pituitary adenylate cyclase-activating polypeptide questions the reproducibility of studies carried out with tumor cell lines.
Neuroendocrinology 113:216-230(2023)

Cross-references
Cell line collections Abcam; ab279978
AddexBio; C0032001/4927
ATCC; CRL-1721
BCRC; 60048
BCRJ; 0203
CCTCC; GDC0006
CLS; 500311
DSMZ; ACC-159
ECACC; 88022401
ICLC; ATL98004
IZSLER; BS TCL 91
JCRB; IFO50278
JCRB; JCRB0733
KCB; KCB 93033YJ
KCB; KCB 200735YJ
KCB; KCB 200736YJ
KCLB; 21721
NCBI_Iran; C153
RCB; RCB0009
Cell line databases/resources CLO; CLO_0008392
CLO; CLO_0050821
MCCL; MCC:0000379
CLDB; cl3876
CLDB; cl3877
CLDB; cl3878
CLDB; cl4922
CLDB; cl5245
CCRID; 1101RAT-PUMC000024
CCRID; 3101RATSCSP517
CCRID; 3101RATTCR3
CCRID; 4201RAT-CCTCC00006
CCRID; 5301RAT-KCB07036YJ
DSMZCellDive; ACC-159
Lonza; 124
TOKU-E; 2867
TOKU-E; 3554
Anatomy/cell type resources BTO; BTO:0001009
Chemistry resources ChEMBL-Cells; CHEMBL3307976
ChEMBL-Targets; CHEMBL612556
PubChem_Cell_line; CVCL_0481
Encyclopedic resources Wikidata; Q7118481
Experimental variables resources EFO; EFO_0001225
Medical resources MeSH; D016716
Entry history
Entry creation04-Apr-2012
Last entry update21-Mar-2023
Version number30