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Cellosaurus PC12 (CVCL_0481)

[Text version]
Cell line name PC12
Synonyms PC-12; PC 12; PC12.1
Accession CVCL_0481
Resource Identification Initiative To cite this cell line use: PC12 (RRID:CVCL_0481)
Comments Group: Space-flown cell line (cellonaut).
Characteristics: Responds reversibly to NGF by induction of a neuronal phenotype.
Doubling time: 40 hours (PubMed=9886495); 92 hours (CLS=500311; ECACC=88022401); ~50-60 hours (DSMZ=ACC-159).
Derived from site: In situ; Adrenal gland; UBERON=UBERON_0002369.
Disease Rat adrenal gland pheochromocytoma (NCIt: C121568)
Species of origin Rattus norvegicus (Rat) (NCBI Taxonomy: 10116)
Breed/subspecies: NEDH; RS=RS_0000603.
Hierarchy Children:
CVCL_0109 (123.7)CVCL_0133 (A126-1B2)CVCL_X983 (A208-4)
CVCL_X984 (A208-7)CVCL_X985 (AB.11)CVCL_YJ89 (LINTERNA PC-12)
CVCL_JY59 (Neuroscreen-1)CVCL_F659 (PC-12 Adh)CVCL_AU97 (PC-12 HD-Q23 cell 14)
CVCL_AU98 (PC-12 HD-Q23 cell 7)CVCL_AU99 (PC-12 HD-Q74 cell 10)CVCL_AV00 (PC-12 HD-Q74 cell 1b)
CVCL_5574 (PC-12TG)CVCL_C0PY (PC12 6c)CVCL_DN60 (PC12 HTT-SW2-Q145 clone 14)
CVCL_DN61 (PC12 HTT-SW2-Q145-TagRFP clone 5)CVCL_DN62 (PC12 HTT-SW2-Q23 clone 28)CVCL_DN63 (PC12 HTT-SW2-Q23-TagRFP clone 34)
CVCL_DN64 (PC12 HTT-SW2-Q73 clone 29)CVCL_DN65 (PC12 HTT-SW2-Q73-TagRFP clone 17)CVCL_V361 (PC12 Tet-Off)
CVCL_V333 (PC12 Tet-On)CVCL_C0PQ (PC12-1)CVCL_C0PR (PC12-15)
CVCL_C0PS (PC12-16A)CVCL_C0PT (PC12-19)CVCL_VH13 (PC12-1G2)
CVCL_C0PU (PC12-21)CVCL_C0PV (PC12-27)CVCL_VH10 (PC12-2B3)
CVCL_C130 (PC12-615)CVCL_J442 (PC12-AC)CVCL_VH11 (PC12-C3)
CVCL_E6BM (PC12-C41)CVCL_VH12 (PC12-D60)CVCL_T752 (PC12-F7)
CVCL_C0PX (PC12-FR)CVCL_T753 (PC12-G11)CVCL_E6BN (PC12-Mn)
CVCL_C4US (PC12-P1D10)CVCL_C4UT (PC12-P1F1)CVCL_C0PP (PC12.4)
CVCL_5573 (PC12.P3)CVCL_4681 (PC12D)CVCL_JX88 (PC12h)
CVCL_J438 (PC12HS)CVCL_J440 (PC12IS)CVCL_4925 (PC12K)
CVCL_J441 (PC12MS)CVCL_C126 (PC12nnr14)CVCL_C127 (PC12nnr3)
CVCL_C128 (PC12nnr5)CVCL_C129 (PC12nnr6)CVCL_0483 (PC2 [Rat pheochromocytoma])
CVCL_7090 (PC4 [Rat pheochromocytoma])CVCL_7091 (PC6)CVCL_E1AL (Ubigene PC12 Gga3 KO)
CVCL_E1AM (Ubigene PC12 Slc38a2 KO)
Sex of cell Male
Age at sampling Age unspecified
Category Cancer cell line
Web pages Info; ATCC; -; https://www.atcc.org/en/support/technical-support/faqs/pc-12-cells-atcc-crl-1721-vs-atcc-crl-1721-1
Info; COPE; -; http://www.cells-talk.com/index.php/page/copelibrary?key=PC12
Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/p/cell-lines-detail-11.html
Info; Wikipedia; -; https://en.wikipedia.org/wiki/PC12_cell
Provider; CH3 Biosystems; 910001; https://ch3biosystems.com/shop/pc12-cell-line/
Publications

PubMed=1065897; DOI=10.1073/pnas.73.7.2424; PMCID=PMC430592
Lloyd A. Greene, Arthur Steven Tischler;
Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor.
Proc. Natl. Acad. Sci. U.S.A. 73:2424-2428(1976)

PubMed=682602
Arthur Steven Tischler, Lloyd A. Greene;
Morphologic and cytochemical properties of a clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor.
Lab. Invest. 39:77-89(1978)

PubMed=6500171; DOI=10.1016/0303-7207(84)90102-3
Cynthia L. Bethea, Susan L. Kozak;
Effect of extracellular matrix on PC 12 cell shape and dopamine processing.
Mol. Cell. Endocrinol. 37:319-329(1984)

PubMed=2493070; DOI=10.1111/j.1471-4159.1989.tb02538.x
Ritsuko Katoh-Semba, Atsuhiko Oohira, Mamoru Sano, Kimi Watanabe, Satoko Kitajima, Shigeo Kashiwamata;
Glycosaminoglycan composition of PC12 pheochromocytoma cells: a comparison with PC12D cells, a new subline of PC12 cells.
J. Neurochem. 52:889-895(1989)

PubMed=2759161; DOI=10.1016/0277-5379(89)90388-X
Ewa Chelmicka-Schorr, Margaret Eve Checinski, Barry Gilbert Wyatt Arnason;
PC12 pheochromocytoma and sympathetic nervous system derived trophic factors augment growth of neuroblastoma.
Eur. J. Cancer Clin. Oncol. 25:1057-1059(1989)

PubMed=2187480; DOI=10.1089/dna.1990.9.221
Susanne R. Muller, Peter D. Sullivan, Dennis O. Clegg, Stuart C. Feinstein;
Efficient transfection and expression of heterologous genes in PC12 cells.
DNA Cell Biol. 9:221-229(1990)

PubMed=8282765; DOI=10.1242/jcs.106.2.611
Kenneth K. Teng, Irene S. Georgieff, John M. Aletta, Jacques Nunez, Michael L. Shelanski, Lloyd A. Greene;
Characterization of a PC12 cell sub-clone (PC12-C41) with enhanced neurite outgrowth capacity: implications for a modulatory role of high molecular weight tau in neuritogenesis.
J. Cell Sci. 106:611-626(1993)

PubMed=9886495; DOI=10.1002/(SICI)1097-4652(199901)178:1<93::AID-JCP12>3.0.CO;2-U
Donald S. Dwyer, Ye Liu, Ronald J. Bradley;
An ethanol-sensitive variant of the PC12 neuronal cell line: sensitivity to alcohol is associated with increased cell adhesion and decreased glucose accumulation.
J. Cell. Physiol. 178:93-101(1999)

PubMed=15541571; DOI=10.1016/j.mce.2003.02.001
Mary J. Eaton, Helene Duplan;
Useful cell lines derived from the adrenal medulla.
Mol. Cell. Endocrinol. 228:39-52(2004)

PubMed=16223334; DOI=10.1290/0411077.1
Darcelle N. Dixon, Rhonda A. Loxley, Anna M. Barron, Susannah Cleary, Jacqueline Kathleen Phillips;
Comparative studies of PC12 and mouse pheochromocytoma-derived rodent cell lines as models for the study of neuroendocrine systems.
In Vitro Cell. Dev. Biol. Anim. 41:197-206(2005)

PubMed=18005394; DOI=10.1111/j.1748-1716.2007.01805.x; PMCID=PMC2663028
Remco H.S. Westerink, Andrew Graham Ewing;
The PC12 cell as model for neurosecretion.
Acta Physiol. 192:273-285(2008)

PubMed=22538498; DOI=10.1159/000338793
Simona Grozinsky-Glasberg, Ilan Shimon, Hadara Rubinfeld;
The role of cell lines in the study of neuroendocrine tumors.
Neuroendocrinology 96:173-187(2012)

PubMed=33171774; DOI=10.3390/ijms21218356; PMCID=PMC7664380
Tada-aki Kudo, Kanako Tominami, Satoshi Izumi, Yohei Hayashi, Takuya Noguchi, Atsushi Matsuzawa, Guang Hong, Junichi Nakai;
Characterization of PC12 cell subclones with different sensitivities to programmed thermal stimulation.
Int. J. Mol. Sci. 21:8356.1-8356.15(2020)

PubMed=34348336; DOI=10.1159/000518337
Colombe Delage, Lou Breard-Mellin, Caroline Theresine, Sephora Simioneck, Benjamin Lefranc, Jerome Leprince, Magalie Benard, David Vaudry;
The heterogeneity of response of PC12 cells from different laboratories to nerve growth factor and pituitary adenylate cyclase-activating polypeptide questions the reproducibility of studies carried out with tumor cell lines.
Neuroendocrinology 113:216-230(2023)

Cross-references
Cell line collections (Providers) Abcam; ab279978
AddexBio; C0032001/4927
ATCC; CRL-1721
BCRC; 60048
BCRJ; 0203
CCTCC; GDC0006
CLS; 500311
DSMZ; ACC-159
ECACC; 88022401
ICLC; ATL98004
IZSLER; BS TCL 91
JCRB; IFO50278
JCRB; JCRB0733
KCB; KCB 200735YJ
KCB; KCB 200736YJ
KCB; KCB 93033YJ
KCLB; 21721
NCBI_Iran; C153
RCB; RCB0009
Ubigene; YC-C047
Cell line databases/resources CLO; CLO_0008392
CLO; CLO_0050821
MCCL; MCC:0000379
CLDB; cl3876
CLDB; cl3877
CLDB; cl3878
CLDB; cl4922
CLDB; cl5245
CCRID; 1101RAT-PUMC000024
CCRID; 3101RATSCSP517
CCRID; 3101RATTCR3
CCRID; 4201RAT-CCTCC00006
CCRID; 5301RAT-KCB07036YJ
DSMZCellDive; ACC-159
Lonza; 124
TOKU-E; 2867
TOKU-E; 3554
Anatomy/cell type resources BTO; BTO_0001009
Chemistry resources ChEMBL-Cells; CHEMBL3307976
ChEMBL-Targets; CHEMBL612556
PubChem_Cell_line; CVCL_0481
Encyclopedic resources Wikidata; Q7118481
Experimental variables resources EFO; EFO_0001225
Medical resources MeSH; D016716
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number35