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Cellosaurus Ishikawa (CVCL_2529)

[Text version]
Cell line name Ishikawa
Synonyms ISHIKAWA; ISHI
Accession CVCL_2529
Resource Identification Initiative To cite this cell line use: Ishikawa (RRID:CVCL_2529)
Comments Part of: ENCODE project common cell types; tier 3.
Population: Japanese.
Doubling time: 36 hours (Note=At 9th passage), 29 hours (Note=At 40th passage), 27 hours (Note=At 50th passage) (DOI=10.1007/978-4-431-53981-0_2); 35.14 +- 2.68 hours (PubMed=23255909).
Omics: Genomics; ChIP-seq; H3K27ac.
Omics: Genomics; Chromatin accessibility; ATAC-seq.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; Array-based CGH.
Omics: Variations; SNP array analysis.
Misspelling: Ischikawaa; Note=Occasionally.
Derived from site: In situ; Endometrium; UBERON=UBERON_0001295.
Sequence variations
  • Mutation; HGNC; HGNC:8979; PIK3R1; Simple; p.Leu570Pro (c.1709T>C); Zygosity=Heterozygous (from child cell line Ishikawa (Heraklio) 02 ER-).
  • Mutation; HGNC; HGNC:9177; POLE; Simple; p.Pro102Ser (c.304C>T); Zygosity=Heterozygous (from child cell line Ishikawa (Heraklio) 02 ER-).
  • Mutation; HGNC; HGNC:9177; POLE; Simple; p.Asn751fs (c.2252delA); Zygosity=Heterozygous (from child cell line Ishikawa (Heraklio) 02 ER-).
  • Mutation; HGNC; HGNC:9588; PTEN; Simple; p.Glu288fs*3 (c.863delA); Zygosity=Heterozygous (from child cell line Ishikawa (Heraklio) 02 ER-).
  • Mutation; HGNC; HGNC:9588; PTEN; Simple; p.Ser302fs*4 (c.906delC); Zygosity=Heterozygous (PubMed=20944090).
  • Mutation; HGNC; HGNC:9588; PTEN; Simple; p.Thr319fs*1 (c.950_953delTACT) (p.VL317fs) (V317fs*3); Zygosity=Heterozygous (PubMed=20944090).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Asp49His (c.145G>C); ClinVar=VCV000135948; Zygosity=Heterozygous (from child cell line Ishikawa (Heraklio) 02 ER-).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Met246Val (c.736A>G); ClinVar=VCV000100815; Zygosity=Heterozygous (PubMed=15901131).
Disease Type I endometrial adenocarcinoma (NCIt: C40145)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_W441 (EIIL)CVCL_N296 (IK-6)CVCL_9996 (IK-90)
CVCL_IR24 (ISH/DDP)CVCL_6543 (Ishikawa (Heraklio) 02 ER-)CVCL_8783 (Ishikawa (London) 02 ER+)
CVCL_W420 (Ishikawa 1-A-12)CVCL_W421 (Ishikawa 1-A-9)CVCL_W422 (Ishikawa 1-C-10)
CVCL_W423 (Ishikawa 1-D-9)CVCL_W424 (Ishikawa 1-E-8)CVCL_W425 (Ishikawa 1-F-6)
CVCL_W426 (Ishikawa 1-H-6)CVCL_W427 (Ishikawa 2-B-12)CVCL_W428 (Ishikawa 2-C-3)
CVCL_W429 (Ishikawa 2-C-7)CVCL_W430 (Ishikawa 2-E-7)CVCL_W431 (Ishikawa 2-F-4)
CVCL_W432 (Ishikawa 3-D-7)CVCL_W433 (Ishikawa 3-D-9)CVCL_D199 (Ishikawa 3-H-12)
CVCL_W434 (Ishikawa 3-H-3)CVCL_W435 (Ishikawa 3-H-4)CVCL_W436 (Ishikawa 3-H-7)
CVCL_C9CC (ISHIKAWA-Luc)CVCL_IJ14 (Ishikawa-RP)
Sex of cell Female
Age at sampling 39Y
Category Cancer cell line
STR profile Source(s): ECACC=99040201; PubMed=25877200

Markers:
AmelogeninX
CSF1PO11,12
D2S133820
D3S135815,17
D5S81810,11
D7S8209,10
D8S117912,16
D13S3179,12
D16S5399
D18S5113,19
D19S43312.2,14
D21S1128
FGA21
Penta D10,11
Penta E11,19
TH019,10
TPOX8
vWA14,17

Run an STR similarity search on this cell line
Web pages Info; ENCODE; -; http://genome.ucsc.edu/ENCODE/protocols/cell/human/Ishikawa_Crawford_protocol.pdf
Publications

PubMed=4031568
Masato Nishida, Kunitake Kasahara, Minoru Kaneko, Hirokazu Iwasaki, Kazuo Hayashi;
Establishment of a new human endometrial adenocarcinoma cell line, Ishikawa cells, containing estrogen and progesterone receptors.
Nihon Sanka Fujinka Gakkai Zasshi 37:1103-1111(1985)

PubMed=10381137; DOI=10.1016/S0304-3835(98)00270-5
Hiroho Ohta, Hideki Sakamoto, Kazuo Satoh;
In vitro effects of gonadotropin-releasing hormone (GnRH) analogue on cancer cell sensitivity to cis-platinum.
Cancer Lett. 134:111-118(1998)

PubMed=12703541; DOI=10.1111/j.1749-0774.2002.tb00105.x
Masato Nishida;
The Ishikawa cells from birth to the present.
Hum. Cell 15:104-117(2002)

DOI=10.1007/978-4-431-53981-0_2
Masato Nishida;
Ishikawa cells: opening of in vitro hormone research on endometrial carcinoma.
(In book chapter) Cell and molecular biology of endometrial carcinoma; Kuramoto, Hiroyuki & Nishida, Masato (eds.); pp.35-58; Springer; Tokyo; Japan (2003)

PubMed=15053063
Yuko Kamata, Junichi Watanabe, Hiroki Hata, Mieko Hamano, Hiroyuki Kuramoto;
Quantitative study on the correlation between p53 gene mutation and its expression in endometrial carcinoma cell lines.
Eur. J. Gynaecol. Oncol. 25:55-60(2004)

PubMed=15901131; DOI=10.1016/j.prp.2005.01.002
Yoshihiro Murai, Shinichi Hayashi, Hiroyuki Takahashi, Koichi Tsuneyama, Yasuo Takano;
Correlation between DNA alterations and p53 and p16 protein expression in cancer cell lines.
Pathol. Res. Pract. 201:109-115(2005)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
S. Michael Rothenberg, Gayatry Mohapatra, Miguel N. Rivera, Daniel Winokur, Patricia Greninger, Mai Nitta, Peter M. Sadow, Gaya Sooriyakumar, Brian W. Brannigan, Matthew J. Ulman, Rushika M. Perera ...Show all 22 authors... , Rui Wang, Angela Tam, Xiao-Jun Ma, Mark Erlander, Dennis C. Sgroi, James W. Rocco, Mark W. Lingen, Ezra E.W. Cohen, David Neil Louis, Jeffrey Settleman, Daniel Arie Haber; Show fewer authors
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=20218740; DOI=10.1177/153303461000900207; PMCID=PMC5120669
Ying-Mei Wang, Da Yang, David Cogdell, Li-Mei Hu, Feng-Xia Xue, Russell Broaddus, Wei Zhang;
Genomic characterization of gene copy-number aberrations in endometrial carcinoma cell lines derived from endometrioid-type endometrial adenocarcinoma.
Technol. Cancer Res. Treat. 9:179-189(2010)

PubMed=20944090; DOI=10.1126/scitranslmed.3001538
Konstantin J. Dedes, Daniel Wetterskog, Ana M. Mendes-Pereira, Rachael Natrajan, Maryou B. Lambros, Felipe Correa Geyer, Radost Vatcheva, Kay Savage, Alan Mackay, Christopher J. Lord, Alan Ashworth, Jorge Sergio Reis-Filho;
PTEN deficiency in endometrioid endometrial adenocarcinomas predicts sensitivity to PARP inhibitors.
Sci. Transl. Med. 2:53ra75.1-53ra75.8(2010)

PubMed=22710073; DOI=10.1016/j.ygyno.2012.06.017; PMCID=PMC3432677
Christopher T. Korch, Monique A. Spillman, Twila A. Jackson, Britta M. Jacobsen, Susan K. Murphy, Bruce Arthur Lessey, Virgil Craig Jordan, Andrew Peter Bradford;
DNA profiling analysis of endometrial and ovarian cell lines reveals misidentification, redundancy and contamination.
Gynecol. Oncol. 127:241-248(2012)

PubMed=23255909; DOI=10.3892/ol.2012.975; PMCID=PMC3525505
Shun-Jun Zhao, Gen-Xia Li, Li Yang, Lei Li, Hong-Yu Li;
Response-specific progestin resistance in a newly characterized Ishikawa human endometrial cancer subcell line resulting from long-term exposure to medroxyprogesterone acetate.
Oncol. Lett. 5:139-144(2013)

PubMed=25877200; DOI=10.1038/nature14397
Ma-Mie Yu, Suresh K. Selvaraj, May M.Y. Liang-Chu, Sahar Aghajani, Matthew Busse, Jean Yuan, Genee Lee, Franklin V. Peale, Christiaan Klijn, Richard Bourgon, Joshua S. Kaminker, Richard M. Neve;
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=36396974; DOI=10.1038/s41598-022-24211-8; PMCID=PMC9672046
Craig M. Rush, Zannel Blanchard, Jacob T. Polaski, Kyle S. Osborne, Krystle Osby, Jeffery M. Vahrenkamp, Chieh-Hsiang Yang, David H. Lum, Christy R. Hagan, Kimberly K. Leslie, Miles A. Pufall ...Show all 13 authors... , Kristina W. Thiel, Jason Gertz; Show fewer authors
Characterization of HCI-EC-23 a novel estrogen- and progesterone-responsive endometrial cancer cell line.
Sci. Rep. 12:19731.1-19731.17(2022)

Cross-references
Cell line collections (Providers) BCRJ; 0364
CLS; 305262 - Discontinued
ECACC; 99040201
Ubigene; YC-D023
Cell line databases/resources CLO; CLO_0037163
cancercelllines; CVCL_2529
CCRID; 1101HUM-PUMC000686
Cell_Model_Passport; SIDM00038
LINCS_HMS; 50018
LINCS_LDP; LCL-1497
TOKU-E; 3966
Anatomy/cell type resources BTO; BTO_0003041
Biological sample resources BioSample; SAMN03471030
ENCODE; ENCBS228AAA
ENCODE; ENCBS229AAA
ENCODE; ENCBS230AAA
Chemistry resources ChEMBL-Cells; CHEMBL3307675
ChEMBL-Targets; CHEMBL614649
PharmacoDB; Ishikawa_667_2019
PubChem_Cell_line; CVCL_2529
Encyclopedic resources Wikidata; Q54898281
Experimental variables resources EFO; EFO_0005718
Gene expression databases ArrayExpress; E-MTAB-3610
GEO; GSM374972
GEO; GSM375431
GEO; GSM827370
GEO; GSM1008593
GEO; GSM1008594
GEO; GSM1669941
GEO; GSM3161714
GEO; GSM3161715
GEO; GSM6421758
GEO; GSM6421759
GEO; GSM6421760
GEO; GSM6421769
GEO; GSM6421770
GEO; GSM6421771
GEO; GSM6421772
GEO; GSM6421773
GEO; GSM6421774
GEO; GSM6421775
GEO; GSM6421776
GEO; GSM6727286
GEO; GSM6727287
Polymorphism and mutation databases Cosmic; 713475
Cosmic; 713499
Cosmic; 809129
Cosmic; 846174
Cosmic; 1070811
Cosmic; 1152553
Cosmic; 1176597
Cosmic; 1177618
Cosmic; 1241344
Cosmic; 1622902
Cosmic; 1696753
Cosmic; 2030475
Cosmic; 2464666
Cosmic; 2646616
Cosmic; 2702440
IARC_TP53; 1112
Progenetix; CVCL_2529
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number38