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Cellosaurus Jurkat E6.1 (CVCL_0367)

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Cell line name Jurkat E6.1
Synonyms JurkatE6-1; Jurkat E6-1; Jurkat, Clone E6-1; Jurkat Clone E6-1; Jurkat (clone E6-1); JURKAT E-6.1; JURKAT E-61; Jurkat-CloneE61; Jurkat-E6; Jurkat E6; J.E6-1; E6-1; Jurkat J6
Accession CVCL_0367
Secondary accession CVCL_4530
Resource Identification Initiative To cite this cell line use: Jurkat E6.1 (RRID:CVCL_0367)
Comments Group: Space-flown cell line (cellonaut).
Part of: Tumor Immunology Bank (TIB) collection from Salk (transferred to ATCC in 1981).
Population: Caucasian.
Doubling time: 20.7 +- 2.2 hours (PubMed=10468210).
Omics: Cell surface proteome.
Omics: Deep phosphoproteome analysis.
Omics: Deep quantitative phosphoproteome analysis.
Omics: Genome sequenced.
Omics: H3K4me3 ChIP-seq epigenome analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis by RNAseq.
Anecdotal: Have been flown in space on shuttle flights STS-76, STS-80 and STS-95 to study apoptosis and cytoskeleton modifications (PubMed=9707173; PubMed=10832058; PubMed=11481229; PubMed=11669127).
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Cell type: T-cell; CL=CL_0000084.
Sequence variations
  • Mutation; HGNC; 959; BAX; Simple; p.Glu41Argfs*19 (c.121delG); ClinVar=VCV000009512; Zygosity=Heterozygous (PubMed=29739316).
  • Mutation; HGNC; 959; BAX; Simple; p.Glu41Glyfs*33 (c.121dupG); ClinVar=VCV000009511; Zygosity=Heterozygous (PubMed=29739316).
  • Mutation; HGNC; 16712; FBXW7; Simple; p.Arg505Cys (c.1513C>T); ClinVar=VCV000069961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 6079; INPP5D; Simple; p.Gln345Ter (c.1033C>T); Zygosity=Heterozygous (PubMed=29739316).
  • Mutation; HGNC; 6079; INPP5D; Simple; c.1097+1065_1097+1112del47; Zygosity=Heterozygous (PubMed=29739316).
  • Mutation; HGNC; 7325; MSH2; Simple; p.Arg711Ter (c.2131C>T); ClinVar=VCV000090903; Zygosity=Homozygous (PubMed=29739316).
  • Mutation; HGNC; 7329; MSH6; Simple; p.Phe1088Serfs*2 (c.3261delC); ClinVar=VCV000089363; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 11491; SYK; Simple; p.Met34Hisfs*3 (c.98_99insG); Zygosity=Heterozygous (PubMed=7592872; PubMed=29739316).
  • Mutation; HGNC; 11998; TP53; Simple; p.Arg196Ter (c.586C>T); ClinVar=VCV000043589; Zygosity=Heterozygous (PubMed=29739316).
HLA typing Source: PubMed=26589293
Class I
Disease Childhood T acute lymphoblastic leukemia (NCIt: C7953)
Precursor T-cell acute lymphoblastic leukemia (ORDO: Orphanet_99861)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0065 (Jurkat)
CVCL_6819 (10B10)CVCL_X694 (1G5 [Human Jurkat derivative])CVCL_A9P6 (Foxp3 reporter (Luc)-Jurkat)
CVCL_J124 (J-beta2.7)CVCL_0354 (J.CaM1.6)CVCL_R862 (J.CaM2)
CVCL_6410 (J.gamma1)CVCL_1316 (J.RT3-T3.5)CVCL_2530 (J45.01)
CVCL_JL96 (JKBulk)CVCL_LH47 (Jurkat 1F2)CVCL_C3CE (Jurkat B2M KO)
CVCL_WN96 (Jurkat E6.1 NF-kappaB::eGFP)CVCL_DS01 (Jurkat J77)CVCL_DF51 (Jurkat NucLight Red)
CVCL_C3EZ (Jurkat Safe Harbor Landing Pad)CVCL_C8Z3 (Jurkat, Clone E6-1-Luc)CVCL_JL98 (JXBulk)
CVCL_A9P4 (NF-kappaB reporter (Luc)-Jurkat)CVCL_6429 (P116)CVCL_D588 (T-REx-Jurkat)
Sex of cell Male
Age at sampling 14Y
Category Cancer cell line
STR profile Source(s): ATCC; CCRID; CLS; ECACC; KCLB; PubMed=11416159; PubMed=25877200; PubMed=32938764

CSF1PO10,11,12,13 (PubMed=32938764)
11,12 (ATCC; CCRID; CLS; ECACC; KCLB; PubMed=25877200)
D3S135815 (CCRID; KCLB)
15,17 (CLS; PubMed=25877200; PubMed=32938764)
D7S8208,10 (CLS; ECACC)
8,12 (ATCC; CCRID; KCLB; PubMed=25877200; PubMed=32938764)
D8S117912,13,14,15 (PubMed=32938764)
13,14 (CCRID; PubMed=11416159; PubMed=25877200)
D18S5112,13,20,21 (PubMed=32938764)
13,21 (CCRID; CLS; PubMed=11416159; PubMed=25877200)
D21S1130.2,31.2,33.2 (PubMed=32938764)
31.2,33.2 (CCRID; CLS; PubMed=11416159; PubMed=25877200)
Penta D11,13
Penta E10,12
vWA18 (ATCC; CCRID; CLS; ECACC; KCLB; PubMed=11416159; PubMed=25877200)
18,19 (PubMed=32938764)

Run an STR similarity search on this cell line
Web pages http://www.nibsc.org/Default.aspx?pageid=597&id=0027&theme=default

PubMed=8316623; DOI=10.2307/3578190
Evans H.H., Ricanati M., Horng M.-F., Jiang Q.-Y., Mencl J., Olive P.L.
DNA double-strand break rejoining deficiency in TK6 and other human B-lymphoblast cell lines.
Radiat. Res. 134:307-315(1993)

PubMed=7592872; DOI=10.1074/jbc.270.44.26533
Fargnoli J., Burkhardt A.L., Laverty M., Kut S.A., van Oers N.S.C., Weiss A., Bolen J.B.
Syk mutation in Jurkat E6-derived clones results in lack of p72syk expression.
J. Biol. Chem. 270:26533-26537(1995)

PubMed=9707173; DOI=10.1096/fasebj.12.11.1007
Lewis M.L., Reynolds J.L., Cubano L.A., Hatton J.P., Lawless B.D., Piepmeier E.H.
Spaceflight alters microtubules and increases apoptosis in human lymphocytes (Jurkat).
FASEB J. 12:1007-1018(1998)

PubMed=10468210; DOI=10.1016/S0891-5849(99)00074-X
Schoene N.W., Kamara K.S.
Population doubling time, phosphatase activity, and hydrogen peroxide generation in Jurkat cells.
Free Radic. Biol. Med. 27:364-369(1999)

PubMed=10832058; DOI=10.1016/S0531-5565(00)00090-5
Cubano L.A., Lewis M.L.
Fas/APO-1 protein is increased in spaceflown lymphocytes (Jurkat).
Exp. Gerontol. 35:389-400(2000)

PubMed=11416159; DOI=10.1073/pnas.121616198
Masters J.R.W., Thomson J.A., Daly-Burns B., Reid Y.A., Dirks W.G., Packer P., Toji L.H., Ohno T., Tanabe H., Arlett C.F., Kelland L.R., Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.
Short tandem repeat profiling provides an international reference standard for human cell lines.
Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)

PubMed=11481229; DOI=10.1096/fj.00-0820fje
Lewis M.L., Cubano L.A., Zhao B.-T., Dinh H.-K., Pabalan J.G., Piepmeier E.H., Bowman P.D.
cDNA microarray reveals altered cytoskeletal gene expression in space-flown leukemic T lymphocytes (Jurkat).
FASEB J. 15:1783-1785(2001)

PubMed=11669127; DOI=10.1016/S0094-5765(01)00116-3
Schatten H., Lewis M.L., Chakrabarti A.
Spaceflight and clinorotation cause cytoskeleton and mitochondria changes and increases in apoptosis in cultured cells.
Acta Astronaut. 49:399-418(2001)

PubMed=15472075; DOI=10.1126/science.1102160
Weng A.P., Ferrando A.A., Lee W., Morris J.P. IV, Silverman L.B., Sanchez-Irizarry C., Blacklow S.C., Look A.T., Aster J.C.
Activating mutations of NOTCH1 in human T cell acute lymphoblastic leukemia.
Science 306:269-271(2004)

PubMed=18842727; DOI=10.1128/JVI.01726-08
Takeuchi Y., McClure M.O., Pizzato M.
Identification of gammaretroviruses constitutively released from cell lines used for human immunodeficiency virus research.
J. Virol. 82:12585-12588(2008)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=23933134; DOI=10.1016/j.jprot.2013.07.019
Storvold G.L., Landskron J., Strozynski M., Arntzen M.O., Koehler C.J., Kalland M.E., Tasken K., Thiede B.
Quantitative profiling of tyrosine phosphorylation revealed changes in the activity of the T cell receptor signaling pathway upon cisplatin-induced apoptosis.
J. Proteomics 91:344-357(2013)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25877200; DOI=10.1038/nature14397
Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=25894527; DOI=10.1371/journal.pone.0121314
Bausch-Fluck D., Hofmann A., Bock T., Frei A.P., Cerciello F., Jacobs A., Moest H., Omasits U., Gundry R.L., Yoon C., Schiess R., Schmidt A., Mirkowska P., Hartlova A.S., Van Eyk J.E., Bourquin J.-P., Aebersold R., Boheler K.R., Zandstra P.W., Wollscheid B.
A mass spectrometric-derived cell surface protein atlas.
PLoS ONE 10:E0121314-E0121314(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5
Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=26546556; DOI=10.1016/j.jprot.2015.10.029
Nguyen T.D., Carrascal M., Vidal-Cortes O., Gallardo O., Casas V., Gay M., Phan V.C., Abian J.
The phosphoproteome of human Jurkat T cell clones upon costimulation with anti-CD3/anti-CD28 antibodies.
J. Proteomics 131:190-198(2016)

PubMed=28464451; DOI=10.1002/pmic.201600470
Tran T.T., Strozynski M., Thiede B.
Quantitative phosphoproteome analysis of cisplatin-induced apoptosis in Jurkat T cells.
Proteomics 17:1600470.1-1600470.11(2017)

PubMed=29739316; DOI=10.1186/s12864-018-4718-6
Gioia L., Siddique A., Head S.R., Salomon D.R., Su A.I.
A genome-wide survey of mutations in the Jurkat cell line.
BMC Genomics 19:334.1-334.13(2018)

PubMed=32938764; DOI=10.1128/JVI.01334-20
Fernandez M.V., Hoffman H.K., Pezeshkian N., Tedbury P.R., van Engelenburg S.B., Freed E.O.
Elucidating the basis for permissivity of the MT-4 T-cell line to replication of an HIV-1 mutant lacking the gp41 cytoplasmic tail.
J. Virol. 94:e01334.20.1-e01334.20.27(2020)

Cell line collections (Providers) ATCC; TIB-152
BCRC; 60424
BCRJ; 0125
CLS; 300223
ECACC; 88042803
HIVReagentProgram; ARP-177
KCB; KCB 94008YJ
KCLB; 40152
NCBI_Iran; C121
Ubigene; YC-D012
Cell line databases/resources CLO; CLO_0007044
CLO; CLO_0007045
MCCL; MCC:0000260
CLDB; cl2962
CLDB; cl5213
cancercelllines; CVCL_0367
CCRID; 1101HUM-PUMC000075
CCRID; 1101HUM-PUMC000348
Cell_Model_Passport; SIDM01244
TOKU-E; 2081
Anatomy/cell type resources BTO; BTO:0001948
Biological sample resources BioSample; SAMN03472194
Chemistry resources ChEMBL-Cells; CHEMBL4295390
ChEMBL-Targets; CHEMBL4296446
PharmacoDB; Jurkat_Clone_E61_713_2019
PubChem_Cell_line; CVCL_0367
Encyclopedic resources Wikidata; Q54899132
Gene expression databases ArrayExpress; E-MTAB-2706
GEO; GSE101101
GEO; GSM472928
GEO; GSM510511
GEO; GSM827151
GEO; GSM923424
GEO; GSM945267
GEO; GSM945268
Metabolomic databases MetaboLights; MTBLS789
Polymorphism and mutation databases Cosmic; 913419
Progenetix; CVCL_0367
Proteomic databases PRIDE; PRD000345
PRIDE; PXD000148
PRIDE; PXD000589
PRIDE; PXD002871
PRIDE; PXD004415
PRIDE; PXD010176
PRIDE; PXD032302
Sequence databases EGA; EGAS00001000610
Entry history
Entry creation04-Apr-2012
Last entry update30-Jan-2024
Version number40