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Cellosaurus J.CaM1.6 (CVCL_0354)

[Text version]
Cell line name J.CaM1.6
Synonyms J CaM 1.6; J-CaM-1.6; JCam-1.6; JCaM 1.6; JCAM 1.6; JCaM1.6; Jcam clone 1.6; J.CaM1; JCaM-1; JCaM1; JCAM1; Jurkat-derived Ca2+ mutant no. 1
Accession CVCL_0354
Resource Identification Initiative To cite this cell line use: J.CaM1.6 (RRID:CVCL_0354)
Comments Population: Caucasian.
Characteristics: Deficient for LCK activity due to a deletion of exon 7.
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Cell type: T-cell; CL=CL_0000084.
Sequence variations
  • Mutation; HGNC; 959; BAX; Simple; p.Glu41Argfs*19 (c.121delG); ClinVar=VCV000009512; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 959; BAX; Simple; p.Glu41Glyfs*33 (c.121dupG); ClinVar=VCV000009511; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 16712; FBXW7; Simple; p.Arg505Cys (c.1513C>T); ClinVar=VCV000069961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 6079; INPP5D; Simple; p.Gln345Ter (c.1033C>T); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 6079; INPP5D; Simple; c.1097+1065_1097+1112del47; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 7325; MSH2; Simple; p.Arg711Ter (c.2131C>T); ClinVar=VCV000090903; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 7329; MSH6; Simple; p.Phe1088Serfs*2 (c.3261delC); ClinVar=VCV000089363; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 11491; SYK; Simple; p.Met34Hisfs*3 (c.98_99insG); Zygosity=Heterozygous (PubMed=7592872).
  • Mutation; HGNC; 11998; TP53; Simple; p.Arg196Ter (c.586C>T); ClinVar=VCV000043589; Zygosity=Heterozygous (from parent cell line).
Disease Childhood T acute lymphoblastic leukemia (NCIt: C7953)
Precursor T-cell acute lymphoblastic leukemia (ORDO: Orphanet_99861)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0367 (Jurkat E6.1)
Sex of cell Male
Age at sampling 14Y
Category Cancer cell line
STR profile Source(s): ATCC; ECACC


Run an STR similarity search on this cell line
Web pages https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/j/cell-lines-detail-80.html

PubMed=3309950; DOI=10.1073/pnas.84.19.6879
Goldsmith M.A., Weiss A.
Isolation and characterization of a T-lymphocyte somatic mutant with altered signal transduction by the antigen receptor.
Proc. Natl. Acad. Sci. U.S.A. 84:6879-6883(1987)

PubMed=3263650; DOI=10.1073/pnas.85.22.8613
Goldsmith M.A., Dazin P.F., Weiss A.
At least two non-antigen-binding molecules are required for signal transduction by the T-cell antigen receptor.
Proc. Natl. Acad. Sci. U.S.A. 85:8613-8617(1988)

PubMed=7592872; DOI=10.1074/jbc.270.44.26533
Fargnoli J., Burkhardt A.L., Laverty M., Kut S.A., van Oers N.S.C., Weiss A., Bolen J.B.
Syk mutation in Jurkat E6-derived clones results in lack of p72syk expression.
J. Biol. Chem. 270:26533-26537(1995)

PubMed=8626636; DOI=10.1074/jbc.271.17.9976
Straus D.B., Chan A.C.-Y., Patai B., Weiss A.
SH2 domain function is essential for the role of the Lck tyrosine kinase in T cell receptor signal transduction.
J. Biol. Chem. 271:9976-9981(1996)

PubMed=11231305; DOI=10.1046/j.1432-1327.2001.02028.x
Livolsi A., Busuttil V., Imbert V., Abraham R.T., Peyron J.-F.
Tyrosine phosphorylation-dependent activation of NF-kappa B Requirement for p56 LCK and ZAP-70 protein tyrosine kinases.
Eur. J. Biochem. 268:1508-1515(2001)

PubMed=33562083; DOI=10.3390/cells10020343
Vico-Barranco I., de Arbulo-Echevarria M.M., Serrano-Garcia I., Perez-Linaza A., Miranda-Sayago J.M., Miazek A., Narbona-Sanchez I., Aguado E.
A novel, LAT/Lck double deficient T cell subline J.CaM1.7 for combined analysis of early TCR signaling.
Cells 10:343.1-343.20(2021)

Cell line collections (Providers) ATCC; CRL-2063
ECACC; 96060401
Cell line databases/resources CLO; CLO_0006983
MCCL; MCC:0000248
CLDB; cl2934
cancercelllines; CVCL_0354
Anatomy/cell type resources BTO; BTO:0003314
Biological sample resources BioSample; SAMN03471427
Encyclopedic resources Wikidata; Q54898427
Entry history
Entry creation04-Apr-2012
Last entry update05-Oct-2023
Version number27