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Cellosaurus AGS (CVCL_0139)

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Cell line name AGS
Accession CVCL_0139
Resource Identification Initiative To cite this cell line use: AGS (RRID:CVCL_0139)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: FGFR genetic alteration cell panel (ATCC TCP-1034).
Part of: JFCR45 cancer cell line panel.
Part of: MD Anderson Cell Lines Project.
Part of: PI3K genetic alteration cell panel (ATCC TCP-1028).
Population: Caucasian.
Virology: Persistently infected with parainfluenza virus type 5 (PIV5) strain PIV5-AGS (PubMed=17509637; PubMed=27445371).
Doubling time: 24 hours (PubMed=1370612); 22 hours (PubMed=25984343); 24.16 +- 0.07 hours (PubMed=35122114); 20 hours (ATCC=CRL-1739).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: ChIP-seq GATA4 analysis.
Omics: ChIP-seq GATA6 analysis.
Omics: ChIP-seq KLF5 analysis.
Omics: CRISPR phenotypic screen.
Omics: Deep exome analysis.
Omics: Deep quantitative proteome analysis.
Omics: DNA methylation analysis.
Omics: Protein expression by reverse-phase protein arrays.
Omics: shRNA library screening.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Derived from site: In situ; Stomach; UBERON=UBERON_0000945.
Sequence variations
  • Mutation; HGNC; 2514; CTNNB1; Simple; p.Gly34Glu (c.101G>A); ClinVar=VCV000017584; Zygosity=Heterozygous (ATCC; Cosmic-CLP; DepMap).
  • Mutation; HGNC; 6407; KRAS; Simple; p.Gly12Asp (c.35G>A); ClinVar=VCV000012582; Zygosity=Heterozygous (Cosmic-CLP; DepMap).
  • Mutation; HGNC; 8975; PIK3CA; Simple; p.Glu453Lys (c.1357G>A); ClinVar=VCV000376470; Zygosity=Heterozygous (Cosmic-CLP; DepMap).
  • Mutation; HGNC; 8975; PIK3CA; Simple; p.Glu545Ala (c.1634A>C); ClinVar=VCV000013659; Zygosity=Heterozygous (Cosmic-CLP; DepMap).
  • Mutation; HGNC; 11998; TP53; None_reported; -; Zygosity=- (PubMed=1370612).
HLA typing Source: PubMed=25960936
Class I
Class II

Source: PubMed=26589293
Class I
Class II
Genome ancestry Source: PubMed=30894373

Origin% genome
Native American32.25
East Asian, North5.38
East Asian, South0
South Asian0.23
European, North27.58
European, South31.12
Disease Gastric adenocarcinoma (NCIt: C4004)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_VP28 (AGS NOD1 KO clone 41A8)CVCL_VP29 (AGS NOD1 KO clone 41H8)CVCL_B7H2 (AGS-5FU-R)
CVCL_VZ57 (mAGS(1))CVCL_VZ58 (mAGS(2))CVCL_VZ59 (mAGS(3))
CVCL_VZ60 (mAGS(4))CVCL_VZ61 (mAGS(5))
Sex of cell Female
Age at sampling 54Y
Category Cancer cell line
STR profile Source(s): AddexBio; ATCC; BCRC; CCRID; CLS; Cosmic-CLP; ECACC; Genomics_Center_BCF_Technion; KCLB

D3S135815.2 (KCLB)
16 (CCRID; CLS; Genomics_Center_BCF_Technion)
Penta D9,10
Penta E13,16

Run an STR similarity search on this cell line
Web pages https://tcpaportal.org/mclp/

Barranco S.C., Townsend C.M. Jr., Casartelli C., Macik B.G., Burger N.L., Boerwinkle W.R., Gourley W.K.
Establishment and characterization of an in vitro model system for human adenocarcinoma of the stomach.
Cancer Res. 43:1703-1709(1983)

PubMed=1370612; DOI=10.1016/S0006-291X(05)80133-0
Matozaki T., Sakamoto C., Matsuda K., Suzuki T., Konda Y., Nakano O., Wada K., Uchida T., Nishisaki H., Nagao M., Kasuga M.
Missense mutations and a deletion of the p53 gene in human gastric cancer.
Biochem. Biophys. Res. Commun. 182:215-223(1992)

Sekiguchi M., Suzuki T.
Gastric tumor cell lines.
(In) Atlas of human tumor cell lines; Hay R.J., Park J.-G., Gazdar A.F. (eds.); pp.287-316; Academic Press; New York (1994)

PubMed=10402490; DOI=10.3892/ijmm.4.2.203
Rha S.Y., Noh S.H., Kim T.S., Yoo N.-C., Roh J.-K., Min J.-S., Kim B.-S.
Modulation of biological phenotypes for tumor growth and metastasis by target-specific biological inhibitors in gastric cancer.
Int. J. Mol. Med. 4:203-212(1999)

PubMed=15767549; DOI=10.1158/1535-7163.MCT-04-0234
Nakatsu N., Yoshida Y., Yamazaki K., Nakamura T., Dan S., Fukui Y., Yamori T.
Chemosensitivity profile of cancer cell lines and identification of genes determining chemosensitivity by an integrated bioinformatical approach using cDNA arrays.
Mol. Cancer Ther. 4:399-412(2005)

PubMed=17509637; DOI=10.1016/j.virol.2007.03.061
Young D.F., Carlos T.S., Hagmaier K., Fan L., Randall R.E.
AGS and other tissue culture cells can unknowingly be persistently infected with PIV5; a virus that blocks interferon signalling by degrading STAT1.
Virology 365:238-240(2007)

PubMed=18804159; DOI=10.1016/j.ygeno.2008.08.002
Jung J.-J., Jeung H.-C., Chung H.C., Lee J.O., Kim T.S., Kim Y.T., Noh S.H., Rha S.Y.
In vitro pharmacogenomic database and chemosensitivity predictive genes in gastric cancer.
Genomics 93:52-61(2009)

PubMed=20164919; DOI=10.1038/nature08768
Bignell G.R., Greenman C.D., Davies H., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=24807215; DOI=10.1038/ncomms4830
Liu J.-F., McCleland M.L., Stawiski E.W., Gnad F., Mayba O., Haverty P.M., Durinck S., Chen Y.-J., Klijn C., Jhunjhunwala S., Lawrence M., Liu H.-B., Wan Y.-N., Chopra V.S., Yaylaoglu M.B., Yuan W.-L., Ha C., Gilbert H.N., Reeder J., Pau G., Stinson J., Stern H.M., Manning G., Wu T.D., Neve R.M., de Sauvage F.J., Modrusan Z., Seshagiri S., Firestein R., Zhang Z.-M.
Integrated exome and transcriptome sequencing reveals ZAK isoform usage in gastric cancer.
Nat. Commun. 5:3830.1-3830.8(2014)

PubMed=25960936; DOI=10.4161/21624011.2014.954893
Boegel S., Lower M., Bukur T., Sahin U., Castle J.C.
A catalog of HLA type, HLA expression, and neo-epitope candidates in human cancer cell lines.
OncoImmunology 3:e954893.1-e954893.12(2014)

PubMed=25984343; DOI=10.1038/sdata.2014.35
Cowley G.S., Weir B.A., Vazquez F., Tamayo P., Scott J.A., Rusin S., East-Seletsky A., Ali L.D., Gerath W.F.J., Pantel S.E., Lizotte P.H., Jiang G.-Z., Hsiao J., Tsherniak A., Dwinell E., Aoyama S., Okamoto M., Harrington W., Gelfand E.T., Green T.M., Tomko M.J., Gopal S., Wong T.C., Li H.-B., Howell S., Stransky N., Liefeld T., Jang D., Bistline J., Meyers B.H., Armstrong S.A., Anderson K.C., Stegmaier K., Reich M., Pellman D., Boehm J.S., Mesirov J.P., Golub T.R., Root D.E., Hahn W.C.
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.
Sci. Data 1:140035-140035(2014)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5
Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=27445371; DOI=10.1128/genomeA.00653-16
Wignall-Fleming E., Young D.F., Goodbourn S., Davison A.J., Randall R.E.
Genome sequence of the parainfluenza virus 5 strain that persistently infects AGS cells.
Genome Announc. 4:e00653.16.1-e00653.16.2(2016)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
Li J., Zhao W., Akbani R., Liu W.-B., Ju Z.-L., Ling S.-Y., Vellano C.P., Roebuck P., Yu Q.-H., Eterovic A.K., Byers L.A., Davies M.A., Deng W.-L., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y.-L., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

PubMed=29717028; DOI=10.1042/BSR20180277
Zhang Y.-Q., Yu S., Zhang Z.-Z., Zhao G., Xu J.
Long non-coding RNA AK096174 promotes cell proliferation and invasion in gastric cancer by regulating WDR66 expression.
Biosci. Rep. 38:BSR20180277.1-BSR20180277.12(2018)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=30971826; DOI=10.1038/s41586-019-1103-9
Behan F.M., Iorio F., Picco G., Goncalves E., Beaver C.M., Migliardi G., Santos R., Rao Y., Sassi F., Pinnelli M., Ansari R., Harper S., Jackson D.A., McRae R., Pooley R., Wilkinson P., van der Meer D.J., Dow D., Buser-Doepner C.A., Bertotti A., Trusolino L., Stronach E.A., Saez-Rodriguez J., Yusa K., Garnett M.J.
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature 568:511-516(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J.G., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023
Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. III, Kalocsay M., Jane-Valbuena J., Gelfand E.T., Schweppe D.K., Jedrychowski M.P., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P.
Quantitative proteomics of the Cancer Cell Line Encyclopedia.
Cell 180:387-402.e16(2020)

Chang H.J., Cho M.-H., Choi M.Y., Lee K.E.
Establishment of a 5-fluorouracil-resistant human gastric cancer cell line and exploration of its biological characteristics.
Res. Square 2022:1302156-1302156(2022)

PubMed=35122114; DOI=10.1007/s00210-022-02217-3
Niapour A., Seyedasli N.
Acquisition of paclitaxel resistance modulates the biological traits of gastric cancer AGS cells and facilitates epithelial to mesenchymal transition and angiogenesis.
Naunyn Schmiedebergs Arch. Pharmacol. 395:515-533(2022)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cell line collections (Providers) AddexBio; C0023004/4958
ATCC; CRL-1739
BCRC; 60102
BCRJ; 0311
CLS; 300408
ECACC; 89090402
IBRC; C10071
KCB; KCB 200708YJ
KCLB; 21739
NCBI_Iran; C131
Ubigene; YC-C003
Cell line databases/resources CLO; CLO_0001686
MCCL; MCC:0000032
CLDB; cl258
CLDB; cl5134
cancercelllines; CVCL_0139
CCRID; 1101HUM-PUMC000480
Cell_Model_Passport; SIDM00850
Cosmic-CLP; 906790
DepMap; ACH-000880
LINCS_HMS; 50004
Lonza; 727
TOKU-E; 522
Anatomy/cell type resources BTO; BTO:0001007
Biological sample resources BioSample; SAMN03471028
BioSample; SAMN03471416
BioSample; SAMN10988305
Chemistry resources ChEMBL-Cells; CHEMBL3308078
ChEMBL-Targets; CHEMBL613860
GDSC; 906790
PharmacoDB; AGS_56_2019
PubChem_Cell_line; CVCL_0139
Encyclopedic resources Wikidata; Q54748713
Experimental variables resources EFO; EFO_0002109
Gene expression databases ArrayExpress; E-MTAB-38
ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-2706
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
GEO; GSM267417
GEO; GSM267424
GEO; GSM267431
GEO; GSM267438
GEO; GSM552354
GEO; GSM562392
GEO; GSM618009
GEO; GSM618010
GEO; GSM618011
GEO; GSM618012
GEO; GSM827400
GEO; GSM828792
GEO; GSM843440
GEO; GSM886864
GEO; GSM887929
GEO; GSM1237672
GEO; GSM1237697
GEO; GSM1250894
GEO; GSM1250895
GEO; GSM1250896
GEO; GSM1250897
GEO; GSM1374389
GEO; GSM1374390
GEO; GSM1669595
Polymorphism and mutation databases Cosmic; 685586
Cosmic; 848149
Cosmic; 868235
Cosmic; 873708
Cosmic; 887255
Cosmic; 888819
Cosmic; 906790
Cosmic; 1067211
Cosmic; 1090463
Cosmic; 1187266
Cosmic; 1294957
Cosmic; 1482073
Cosmic; 1518239
Cosmic; 1582429
Cosmic; 1627261
Cosmic; 1995340
Cosmic; 2036659
Cosmic; 2069770
Cosmic; 2484954
Cosmic; 2823227
IARC_TP53; 21171
LiGeA; CCLE_280
Progenetix; CVCL_0139
Proteomic databases PRIDE; PXD000722
PRIDE; PXD030304
Sequence databases EGA; EGAS00001000610
EGA; EGAS00001000978
EGA; EGAS00001002554
Entry history
Entry creation04-Apr-2012
Last entry update05-Oct-2023
Version number43