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Cellosaurus S2R+-MT::Cas9 (CVCL_UD30)

[Text version]
Cell line name S2R+-MT::Cas9
Accession CVCL_UD30
Resource Identification Initiative To cite this cell line use: S2R+-MT::Cas9 (RRID:CVCL_UD30)
Comments Group: Insect cell line.
Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Species of origin Drosophila melanogaster (Fruit fly) (NCBI Taxonomy: 7227)
Breed/subspecies: Oregon-R.
Hierarchy Parent: CVCL_0Q79 (S2R+-NPT005)
Children:
CVCL_UD31 (S2R+-Apc2-KO)CVCL_XF46 (S2R+-Apc-KO-1G)CVCL_XF47 (S2R+-Apc-KO-3E)
CVCL_A5UG (S2R+-Apc-KO-E5)CVCL_XF61 (S2R+-arl8-GFP-4)CVCL_XF62 (S2R+-Cnx99a-GFP-4)
CVCL_XF63 (S2R+-Fib-GFP-11)CVCL_XF64 (S2R+-Fib-GFP-12)CVCL_XF66 (S2R+-Gmap-GFP-4)
CVCL_XF67 (S2R+-Gmap-GFP-7)CVCL_XF68 (S2R+-Golgin245-GFP-1)CVCL_XF49 (S2R+-His2Av-mNeonGreen B11)
CVCL_XF69 (S2R+-Lam-GFP-7)CVCL_XF50 (S2R+-Lamin-mNeonGreen D6)CVCL_A5UF (S2R+-Moe-KO-21B4)
CVCL_A5UI (S2R+-Pex19-KO-A1)CVCL_UZ54 (S2R+-polo-GFP-2)CVCL_A5UJ (S2R+-Pten-KO-1A4)
CVCL_UW09 (S2R+-Rab11-GFP-14)CVCL_XF70 (S2R+-Sec23-GFP-6)CVCL_A5UK (S2R+-Slik-KO-22C2)
CVCL_XF71 (S2R+-spin-GFP-2)CVCL_XF72 (S2R+-Tnks-KO-10B)CVCL_XF73 (S2R+-Tnks-KO-12A)
CVCL_XF74 (S2R+-Tnks-KO-3H)CVCL_XF75 (S2R+-Tom20-GFP-2)CVCL_A5UH (S2R+-TSC1-KO-C5)
CVCL_A5UL (S2R+-Yki-KO-G7)
Sex of cell Male
Age at sampling Late embryonic stage
Category Spontaneously immortalized cell line
Publications

PubMed=32958931; DOI=10.1038/s41596-020-0383-8
Xia B.-L., Amador G., Viswanatha R., Zirin J., Mohr S.E., Perrimon N.
CRISPR-based engineering of gene knockout cells by homology-directed insertion in polyploid Drosophila S2R+ cells.
Nat. Protoc. 15:3478-3498(2020)

Cross-references
Cell line collections DGRC; 268
Cell line databases/resources FlyBase_Cell_line; FBtc0000268
Encyclopedic resources Wikidata; Q98129282
Entry history
Entry creation13-Nov-2018
Last entry update21-Mar-2023
Version number6