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Cellosaurus S2R+-MT::Cas9 (CVCL_UD30)

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Cell line name S2R+-MT::Cas9
Accession CVCL_UD30
Resource Identification Initiative To cite this cell line use: S2R+-MT::Cas9 (RRID:CVCL_UD30)
Comments Group: Insect cell line.
Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Species of origin Drosophila melanogaster (Fruit fly) (NCBI Taxonomy: 7227)
Breed/subspecies: Oregon-R.
Hierarchy Parent: CVCL_0Q79 (S2R+-NPT005)
Children:
CVCL_UD31 (S2R+-Apc2-KO )CVCL_XF46 (S2R+-Apc-KO-1G )CVCL_XF47 (S2R+-Apc-KO-3E )
CVCL_A5UG (S2R+-Apc-KO-E5 )CVCL_XF61 (S2R+-arl8-GFP-4 )CVCL_XF62 (S2R+-Cnx99a-GFP-4 )
CVCL_XF63 (S2R+-Fib-GFP-11 )CVCL_XF64 (S2R+-Fib-GFP-12 )CVCL_XF66 (S2R+-Gmap-GFP-4 )
CVCL_XF67 (S2R+-Gmap-GFP-7 )CVCL_XF68 (S2R+-Golgin245-GFP-1 )CVCL_XF49 (S2R+-His2Av-mNeonGreen B11 )
CVCL_XF69 (S2R+-Lam-GFP-7 )CVCL_XF50 (S2R+-Lamin-mNeonGreen D6 )CVCL_A5UF (S2R+-Moe-KO-21B4 )
CVCL_A5UI (S2R+-Pex19-KO-A1 )CVCL_UZ54 (S2R+-polo-GFP-2 )CVCL_A5UJ (S2R+-Pten-KO-1A4 )
CVCL_UW09 (S2R+-Rab11-GFP-14 )CVCL_XF70 (S2R+-Sec23-GFP-6 )CVCL_A5UK (S2R+-Slik-KO-22C2 )
CVCL_XF71 (S2R+-spin-GFP-2 )CVCL_XF72 (S2R+-Tnks-KO-10B )CVCL_XF73 (S2R+-Tnks-KO-12A )
CVCL_XF74 (S2R+-Tnks-KO-3H )CVCL_XF75 (S2R+-Tom20-GFP-2 )CVCL_A5UH (S2R+-TSC1-KO-C5 )
CVCL_A5UL (S2R+-Yki-KO-G7 )
Sex of cell Male
Age at sampling Late embryonic stage
Category Spontaneously immortalized cell line
Publications

PubMed=32958931; DOI=10.1038/s41596-020-0383-8
Xia B.-L., Amador G., Viswanatha R., Zirin J., Mohr S.E., Perrimon N.
CRISPR-based engineering of gene knockout cells by homology-directed insertion in polyploid Drosophila S2R+ cells.
Nat. Protoc. 15:3478-3498(2020)

Cross-references
Cell line collections DGRC; 268
Cell line databases/resources FlyBase_Cell_line; FBtc0000268
Encyclopedic resources Wikidata; Q98129282
Entry history
Entry creation13-Nov-2018
Last entry update16-Dec-2021
Version number5