Cellosaurus cell line S2R+-MT::Cas9 (CVCL

Cellosaurus S2R+-MT::Cas9 (CVCL_UD30)

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Cell line name

S2R+-MT::Cas9

Accession

CVCL_UD30

Resource Identification Initiative

To cite this cell line use: S2R+-MT::Cas9 (RRID:CVCL_UD30)

Comments

Group: Insect cell line.
Characteristics: Transfected with a pMT-FLAG-Cas9 construct where the expression of N-terminally FLAG-tagged Cas9 is driven by a copper-inducible metallothionein promoter (DGRC=268).
Genetic integration: Method=Protein trap; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transfection; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With a N-termial FLAG tag).
Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.

Species of origin

Drosophila melanogaster (Fruit fly) (NCBI Taxonomy: 7227)
Breed/subspecies: Oregon-R; FlyBase_Strain=FBsn0000276.

Hierarchy

Parent: CVCL_0Q79 (S2R+-NPT005)
Children:

CVCL_UD31 (S2R+-Apc2-KO)	CVCL_XF46 (S2R+-Apc-KO-1G)	CVCL_XF47 (S2R+-Apc-KO-3E)
CVCL_A5UG (S2R+-Apc-KO-E5)	CVCL_XF61 (S2R+-arl8-GFP-4)	CVCL_XF62 (S2R+-Cnx99a-GFP-4)
CVCL_XF63 (S2R+-Fib-GFP-11)	CVCL_XF64 (S2R+-Fib-GFP-12)	CVCL_XF66 (S2R+-Gmap-GFP-4)
CVCL_XF67 (S2R+-Gmap-GFP-7)	CVCL_XF68 (S2R+-Golgin245-GFP-1)	CVCL_XF49 (S2R+-His2Av-mNeonGreen B11)
CVCL_XF69 (S2R+-Lam-GFP-7)	CVCL_XF50 (S2R+-Lamin-mNeonGreen D6)	CVCL_A5UF (S2R+-Moe-KO-21B4)
CVCL_D5B3 (S2R+-msl2-KO)	CVCL_A5UI (S2R+-Pex19-KO-A1)	CVCL_UZ54 (S2R+-polo-GFP-2)
CVCL_A5UJ (S2R+-Pten-KO-1A4)	CVCL_UW09 (S2R+-Rab11-GFP-14)	CVCL_XF70 (S2R+-Sec23-GFP-6)
CVCL_A5UK (S2R+-Slik-KO-22C2)	CVCL_XF71 (S2R+-spin-GFP-2)	CVCL_XF72 (S2R+-Tnks-KO-10B)
CVCL_XF73 (S2R+-Tnks-KO-12A)	CVCL_XF74 (S2R+-Tnks-KO-3H)	CVCL_XF75 (S2R+-Tom20-GFP-2)
CVCL_A5UH (S2R+-TSC1-KO-C5)	CVCL_A5UL (S2R+-Yki-KO-G7)

Sex of cell

Male

Age at sampling

Late embryonic stage