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Cellosaurus S2R+-polo-GFP-2 (CVCL_UZ54)

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Cell line name S2R+-polo-GFP-2
Synonyms polo-GFP-2/Cas9
Accession CVCL_UZ54
Resource Identification Initiative To cite this cell line use: S2R+-polo-GFP-2 (RRID:CVCL_UZ54)
Comments Group: Insect cell line.
From: Drosophila RNAi Screening Center (DRSC), Harvard Medical School; Boston; USA.
Characteristics: The constitutively Cas9-expressed parent cell line was modified with a knock-in of eGFP into the Polo locus (FlyBase_Gene=FBgn0003124).
Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
Species of origin Drosophila melanogaster (Fruit fly) (NCBI Taxonomy: 7227)
Breed/subspecies: Oregon-R.
Hierarchy Parent: CVCL_UD30 (S2R+-MT::Cas9)
Sex of cell Male
Age at sampling Late embryonic stage
Category Spontaneously immortalized cell line
Cell line collections (Providers) DGRC; 275
Cell line databases/resources FlyBase_Cell_line; FBtc0000275
Encyclopedic resources Wikidata; Q98129283
Entry history
Entry creation25-Feb-2019
Last entry update30-Jan-2024
Version number8