Cellosaurus S2R+-polo-GFP-2 (CVCL_UZ54)
| Cell line name | S2R+-polo-GFP-2 |
|---|---|
| Synonyms | polo-GFP-2/Cas9 |
| Accession | CVCL_UZ54 |
| Resource Identification Initiative | To cite this cell line use: S2R+-polo-GFP-2 (RRID:CVCL_UZ54) |
| Comments | Group: Insect cell line. From: Drosophila RNAi Screening Center (DRSC), Harvard Medical School; Boston; USA. Characteristics: The constitutively Cas9-expressed parent cell line was modified with a knock-in of eGFP into the Polo locus (FlyBase_Gene=FBgn0003124). Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9. Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922. |
| Species of origin | Drosophila melanogaster (Fruit fly)
(NCBI Taxonomy: 7227)
Breed/subspecies: Oregon-R; FlyBase_Strain=FBsn0000276. |
| Hierarchy | Parent: CVCL_UD30 (S2R+-MT::Cas9) |
| Sex of cell | Male |
| Age at sampling | Late embryonic stage |
| Category | Spontaneously immortalized cell line |
| Cross-references | |
| Cell line collections (Providers) | DGRC; 275 |
| Cell line databases/resources | FlyBase_Cell_line; FBtc0000275 |
| Encyclopedic resources | Wikidata; Q98129283 |
| Entry history | |
| Entry creation | 25-Feb-2019 |
| Last entry update | 10-Apr-2025 |
| Version number | 10 |