ID   S2R+-polo-GFP-2
AC   CVCL_UZ54
SY   polo-GFP-2/Cas9
DR   DGRC; 275
DR   FlyBase_Cell_line; FBtc0000275
DR   Wikidata; Q98129283
CC   Group: Insect cell line.
CC   From: Drosophila RNAi Screening Center (DRSC), Harvard Medical School; Boston; USA.
CC   Characteristics: The constitutively Cas9-expressed parent cell line was modified with a knock-in of eGFP into the Polo locus (FlyBase_Gene=FBgn0003124).
CC   Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
CC   Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC   Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
CC   Breed/subspecies: Oregon-R.
OX   NCBI_TaxID=7227; ! Drosophila melanogaster (Fruit fly)
HI   CVCL_UD30 ! S2R+-MT::Cas9
SX   Male
AG   Late embryonic stage
CA   Spontaneously immortalized cell line
DT   Created: 25-02-19; Last updated: 30-01-24; Version: 8
//