Cellosaurus S2R+-Tnks-KO-3H (CVCL_XF74)
| Cell line name | S2R+-Tnks-KO-3H |
|---|---|
| Accession | CVCL_XF74 |
| Resource Identification Initiative | To cite this cell line use: S2R+-Tnks-KO-3H (RRID:CVCL_XF74) |
| Comments | Group: Insect cell line. From: Drosophila RNAi Screening Center (DRSC), Harvard Medical School; Boston; USA. Characteristics: Using CRISPR/Cas9 Tnks alleles were replaced with an insertion cassette carrying PuroR. Characteristics: Transfected with a pMT-FLAG-Cas9 construct where the expression of N-terminally FLAG-tagged Cas9 is driven by a copper-inducible metallothionein promoter (from parent cell line). Knockout cell: Method=CRISPR/Cas9; FlyBase_Gene; FBgn0027508; Tnks. Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR). Genetic integration: Method=Protein trap; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)). Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Genetic integration: Method=Transfection; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With a N-termial FLAG tag). Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922. |
| Species of origin | Drosophila melanogaster (Fruit fly)
(NCBI Taxonomy: 7227)
Breed/subspecies: Oregon-R; FlyBase_Strain=FBsn0000276. |
| Hierarchy | Parent: CVCL_UD30 (S2R+-MT::Cas9) |
| Sex of cell | Male |
| Age at sampling | Late embryonic stage |
| Category | Spontaneously immortalized cell line |
| Publications | PubMed=32958931; DOI=10.1038/s41596-020-0383-8; PMCID=PMC7961850 |
| Cross-references | |
| Cell line collections (Providers) | DGRC; 301 |
| Cell line databases/resources | FlyBase_Cell_line; FBtc0000301 |
| Encyclopedic resources | Wikidata; Q98129292 |
| Entry history | |
| Entry creation | 06-Sep-2019 |
| Last entry update | 14-Aug-2025 |
| Version number | 10 |