Cellosaurus S2R+-Tnks-KO-12A (CVCL_XF73)
| Cell line name | S2R+-Tnks-KO-12A |
|---|---|
| Accession | CVCL_XF73 |
| Resource Identification Initiative | To cite this cell line use: S2R+-Tnks-KO-12A (RRID:CVCL_XF73) |
| Comments | Group: Insect cell line. From: Drosophila RNAi Screening Center (DRSC), Harvard Medical School; Boston; USA. Characteristics: Using CRISPR/Cas9 Tnks alleles were replaced with an insertion cassette carrying PuroR. Knockout cell: Method=CRISPR/Cas9; FlyBase_Gene; FBgn0027508; Tnks. Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9. Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922. |
| Species of origin | Drosophila melanogaster (Fruit fly)
(NCBI Taxonomy: 7227)
Breed/subspecies: Oregon-R; FlyBase_Strain=FBsn0000276. |
| Hierarchy | Parent: CVCL_UD30 (S2R+-MT::Cas9) |
| Sex of cell | Male |
| Age at sampling | Late embryonic stage |
| Category | Spontaneously immortalized cell line |
| Publications | PubMed=32958931; DOI=10.1038/s41596-020-0383-8; PMCID=PMC7961850 |
| Cross-references | |
| Cell line collections (Providers) | DGRC; 299 |
| Cell line databases/resources | FlyBase_Cell_line; FBtc0000299 |
| Encyclopedic resources | Wikidata; Q98129291 |
| Entry history | |
| Entry creation | 06-Sep-2019 |
| Last entry update | 10-Sep-2024 |
| Version number | 9 |