ID   S2R+-Tnks-KO-12A
AC   CVCL_XF73
DR   DGRC; 299
DR   FlyBase_Cell_line; FBtc0000299
DR   Wikidata; Q98129291
RX   PubMed=32958931;
CC   Group: Insect cell line.
CC   From: Drosophila RNAi Screening Center (DRSC), Harvard Medical School; Boston; USA.
CC   Characteristics: Using CRISPR/Cas9 Tnks alleles were replaced with an insertion cassette carrying PuroR.
CC   Characteristics: Transfected with a pMT-FLAG-Cas9 construct where the expression of N-terminally FLAG-tagged Cas9 is driven by a copper-inducible metallothionein promoter (from parent cell line).
CC   Knockout cell: Method=CRISPR/Cas9; FlyBase_Gene; FBgn0027508; Tnks.
CC   Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
CC   Genetic integration: Method=Protein trap; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With a N-termial FLAG tag).
CC   Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
CC   Breed/subspecies: Oregon-R; FlyBase_Strain=FBsn0000276.
OX   NCBI_TaxID=7227; ! Drosophila melanogaster (Fruit fly)
HI   CVCL_UD30 ! S2R+-MT::Cas9
SX   Male
AG   Late embryonic stage
CA   Spontaneously immortalized cell line
DT   Created: 06-09-19; Last updated: 14-08-25; Version: 10
//
RX   PubMed=32958931; DOI=10.1038/s41596-020-0383-8; PMCID=PMC7961850;
RA   Xia, Bao-Long
RA   Amador, Gabriel
RA   Viswanatha, Raghuvir
RA   Zirin, Jonathan D.
RA   Mohr, Stephanie E.
RA   Perrimon, Norbert
RT   "CRISPR-based engineering of gene knockout cells by homology-directed
RT   insertion in polyploid Drosophila S2R+ cells.";
RL   Nat. Protoc. 15:3478-3498(2020).
//