ID   S2R+-Tnks-KO-12A
DR   DGRC; 299
DR   FlyBase_Cell_line; FBtc0000299
DR   Wikidata; Q98129291
RX   PubMed=32958931;
CC   Group: Insect cell line.
CC   From: Drosophila RNAi Screening Center (DRSC), Harvard Medical School; Boston; USA.
CC   Characteristics: Using CRISPR/Cas Tnks alleles were replaced with an insertion cassette carrying the puromycin resistance gene.
CC   Knockout cell: Method=CRISPR/Cas9; FlyBase_Gene; FBgn0027508; Tnks.
CC   Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
CC   Breed/subspecies: Oregon-R.
OX   NCBI_TaxID=7227; ! Drosophila melanogaster (Fruit fly)
HI   CVCL_UD30 ! S2R+-MT::Cas9
SX   Male
AG   Late embryonic stage
CA   Spontaneously immortalized cell line
DT   Created: 06-09-19; Last updated: 21-03-23; Version: 6
RX   PubMed=32958931; DOI=10.1038/s41596-020-0383-8;
RA   Xia B.-L., Amador G., Viswanatha R., Zirin J., Mohr S.E., Perrimon N.;
RT   "CRISPR-based engineering of gene knockout cells by homology-directed
RT   insertion in polyploid Drosophila S2R+ cells.";
RL   Nat. Protoc. 15:3478-3498(2020).