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Cellosaurus S2R+-Tnks-KO-10B (CVCL_XF72)

[Text version]
Cell line name S2R+-Tnks-KO-10B
Accession CVCL_XF72
Resource Identification Initiative To cite this cell line use: S2R+-Tnks-KO-10B (RRID:CVCL_XF72)
Comments Group: Insect cell line.
From: Drosophila RNAi Screening Center (DRSC), Harvard Medical School; Boston; USA.
Characteristics: Using CRISPR/Cas Tnks alleles were replaced with an insertion cassette carrying the puromycin resistance gene.
Knockout cell: Method=CRISPR/Cas9; FlyBase_Gene; FBgn0027508; Tnks.
Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR).
Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Species of origin Drosophila melanogaster (Fruit fly) (NCBI Taxonomy: 7227)
Breed/subspecies: Oregon-R.
Hierarchy Parent: CVCL_UD30 (S2R+-MT::Cas9)
Sex of cell Male
Age at sampling Late embryonic stage
Category Spontaneously immortalized cell line

PubMed=32958931; DOI=10.1038/s41596-020-0383-8
Xia B.-L., Amador G., Viswanatha R., Zirin J., Mohr S.E., Perrimon N.
CRISPR-based engineering of gene knockout cells by homology-directed insertion in polyploid Drosophila S2R+ cells.
Nat. Protoc. 15:3478-3498(2020)

Cell line collections DGRC; 300
Cell line databases/resources FlyBase_Cell_line; FBtc0000300
Encyclopedic resources Wikidata; Q98129290
Entry history
Entry creation06-Sep-2019
Last entry update21-Mar-2023
Version number6