Cellosaurus S2R+-His2Av-mNeonGreen B11 (CVCL_XF49)
| Cell line name | S2R+-His2Av-mNeonGreen B11 |
|---|---|
| Accession | CVCL_XF49 |
| Resource Identification Initiative | To cite this cell line use: S2R+-His2Av-mNeonGreen B11 (RRID:CVCL_XF49) |
| Comments | Group: Insect cell line. From: Drosophila RNAi Screening Center (DRSC), Harvard Medical School; Boston; USA. Characteristics: The constitutively Cas9-expressed parent cell line was modified with a knock-in of neonGreen into the His2AV locus (FlyBase_Gene=FBgn0001197). Characteristics: Transfected with a pMT-FLAG-Cas9 construct where the expression of N-terminally FLAG-tagged Cas9 is driven by a copper-inducible metallothionein promoter (from parent cell line). Genetic integration: Method=Protein trap; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)). Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Genetic integration: Method=Transfection; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With a N-termial FLAG tag). Genetic integration: Method=Transfection/transduction; Gene=FPbase; ZRKRV; mNeonGreen (Note=Derivative of Branchiostoma lanceolatum LanYFP). Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922. |
| Species of origin | Drosophila melanogaster (Fruit fly)
(NCBI Taxonomy: 7227)
Breed/subspecies: Oregon-R; FlyBase_Strain=FBsn0000276. |
| Hierarchy | Parent: CVCL_UD30 (S2R+-MT::Cas9) |
| Sex of cell | Male |
| Age at sampling | Late embryonic stage |
| Category | Spontaneously immortalized cell line |
| Cross-references | |
| Cell line collections (Providers) | DGRC; 295 |
| Cell line databases/resources | FlyBase_Cell_line; FBtc0000295 |
| Encyclopedic resources | Wikidata; Q98129278 |
| Entry history | |
| Entry creation | 12-Mar-2020 |
| Last entry update | 14-Aug-2025 |
| Version number | 9 |