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Cellosaurus S2R+-His2Av-mNeonGreen B11 (CVCL_XF49)

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Cell line name S2R+-His2Av-mNeonGreen B11
Accession CVCL_XF49
Resource Identification Initiative To cite this cell line use: S2R+-His2Av-mNeonGreen B11 (RRID:CVCL_XF49)
Comments Group: Insect cell line.
From: Drosophila RNAi Screening Center (DRSC), Harvard Medical School; Boston; USA.
Characteristics: The constitutively Cas9-expressed parent cell line was modified with a knock-in of neonGreen into the His2AV locus (FBgn0001197).
Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Transfected with: UniProtKB; A0A1S4NYF2; mNeonGreen (derivative of Branchiostoma lanceolatum blFP-Y3).
Species of origin Drosophila melanogaster (Fruit fly) (NCBI Taxonomy: 7227)
Breed/subspecies: Oregon-R.
Hierarchy Parent: CVCL_UD30 (S2R+-MT::Cas9)
Sex of cell Male
Age at sampling Late embryonic stage
Category Spontaneously immortalized cell line
Cell line collections DGRC; 295
Cell line databases/resources FlyBase_Cell_line; FBtc0000295
Encyclopedic resources Wikidata; Q98129278
Entry history
Entry creation12-Mar-2020
Last entry update16-Dec-2021
Version number3